Abstract
Sympathetic postganglionic cells of the stellate ganglion (SG) projecting to the skin in young dogs were labeled retrogradely with wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP). After application of WGA-HRP into the dermis and/or subcutaneous tissue of the upper extremity, numerous labeled cells were observed in the SG. The sympathetic postganglionic fibers from the SG were distributed in the skin area between the third cervical vertebra and the level of the thirteenth rib. These fibers decreased in number toward the rostral and caudal skin area with a peak on the upper extremity. Sympathetic postganglionic cells in the SG projecting to these skin areas were found ipsilaterally to the injection side. No labeled cells were observed in the SG after injecting WGA-HRP into the epidermis and/or subcutaneous tissue of the face, the level of the second cervical vertebra, and the lower extremity.
Sympathetic postganglionic neurons of the SG which project in the cervical and thoracic sympathetic trunks were labeled retrogradely with unconjugated horseradish peroxidase (HRP) applied to the cut end of the sympathetic trunks proximal to the SG. The application was made in the cervical sympathetic trunk (CST) caudal to the superior cervical ganglion (SCG). In the thoracic sympathetic trunk (TST), the application was made between the fourth and fifth thoracic sympathetic ganglia (T4 and T5), and between T10 and T11. Labeled cell bodies were observed in SG bilaterally.
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References
Pereira ADS (1945) Blocking of the middle cervical and stellate ganglions with descending infiltration anesthesia: technic, accidents and therapeutic indications. Arch Surg 50: 153–165
Smith DW (1951) Stellate ganglion block: the tissue displacement method. Am J Surg 82: 344–348
Moore DC (1954) Stellate ganglion block. Thomas, Springfield, pp 5–40, 65–79, 96–107
Bonica JJ (1990) The management of pain. Lea and Febiger, Philadelphia, pp 1931–1946
Yamamuro M, Kaneko T (1977) The comparison of stellate ganglion block at the transverse process of the 7th and the 6th cervical vertebra. Masui 27: 376–389
Yatsushiro R, Niki H, Yogi H, et al. (1980) Effects of stellate ganglion block on skin temperature: thermographic observations. Pain Clinic 1: 269–275
Kawabata M, Itou T, Suda T, et al. (1986) Effects of steallate ganglion block with thermography. Pain Clinic 7: 749–752
Drummond PD, Finch PM (1989) Reflex control of facial flushing during body heating in man. Brain 112: 1351–1358
Matsumoto S (1991) Thermographic assessments of the sympathetic blockade by stellate ganglion block (No. 1)—comparison between C7-SGB and C6-SGB in 40 patients—. Masui 40: 562–9
Matsumoto S (1991) Thermographic assessments of the sympathetic blockade by stellate ganglion block (No. 2)—comparison and analysis of thermographic patterns between C7-SGB and C6-SGB in 20 healthy volunteers—. Masui 40: 692–701
Matsumoto S, Mitsuhata H, Hasegawa J (1992) Thermographic assessments of the sympathetic blockade after stellate ganglion block (No. 3)—the block at the base of 6th cervical transverse process—. Masui 41: 111–118
Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117
Fujii M, Kusama T (1984) Fixation of horseradish peroxidase reaction products with ammonium molybdate. Neurosci Res 1: 153–156
Montagna W, Prakkal PF (1974) The structure and function of skin, 3rd edn. Academic, New York, pp 157–171
Fitzpatrick TB (1979). Dermatology in general medicine, 2nd edn. McGraw-Hill, new York, pp 41–69
Evans HE, Christensen GC (1976) Miller's anatomy of the dog. Saunders, Philadelphia, pp 78–100, 652–756
Broadwell RD, Brightman MW (1976) Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood. J Comp Neurol 166: 257–284
Kuntz A (1927) Distribution of the sympathetic rami to the brachial plexus: Its relation to sympathectomy affecting the upper extremity. Arch Surg 15: 871–877
Kirgis HD, Kuntz A (1942) Inconstant sympathetic neural pathways; Their relation to sympathetic denervation of the upper extremity. Arch Surg 44: 95–102
Widenfalk B, Elfvin LG, Wiberg M (1988) Origin of sympathetic and sensory innervation of the elbow joint in the rat: a retrograde axonal tracing study with wheat germ agglutinin conjugated horseradish peroxidase. J Comp Neurol 271: 313–318
Marfurt CF, Zaleski EM, Adams CE, et al. (1986) Sympathetic nerve fibers in rat orofacial and cerebral tissues as revealed by the HRP-WGA tracing technique; a light and electron microscopic study. Brain Res 366: 373–378
Murata Y, Shibata H, Chiba T (1982) A correlative quantitative study comparing the nerve fibers in the cervical sympathetic trunk and the locus of the somata from which they originate in the rat. J Auton Nerv Syst 6: 323–333
Sacchi O, Rossi ML (1981) Cholinergic through-fibers in the rat superiorccervical ganglion. J Auton Nerv Syst 4: 101–106
Bowers CW, Zigmond RE (1981) Sympathetic neurons in lower cervical ganglia send axons through the superior cervical ganglion. Neurosci 6: 1783–1791
Pilowsky P, Llewellyn-Smith IJ, Minson J, et al. (1992) Sympathetic preganglionic neurons in rabbit spinal cord that project to the stellate or the superior cervical ganglion. Brain Res 577: 181–188
Alexander FAD, Lovell BK (1952) Roentgenologic control of nerve blocks; use of iodopyracet injection (Diodrast). JAMA 148: 885–886
Jänig W, McLachlan EM (1986) The sympathetic and sensory components of the caudal lumbar sympathetic trunk in the cat. J Comp Neurol 245: 62–73
Foley JO, DuBois FS (1940) A quantitative and experimental study of the cervical sympathetic trunk. J Comp Neurol 72: 587–603
Foley JO (1945) The components of the cervical sympathetic trunk with special reference to its accessory cells and ganglia. J Comp Neurol 82: 77–91
Oldfield BJ, McLachlan EM (1978) Localization of sensory neurons traversing the stellate ganglion of the cat. J Comp Neurol 182: 915–922
Douglas WW, Ritchie JM (1956) The conduction of impulses through the superior cervical and accessory cervical ganglia of the rabbit. J Physiol 133: 220–231
Bowers CW, Zigmond RE (1979) Localization of neurons in the rat superior cervical ganglion that project into different postganglionic trunks. J Comp Neurol 185: 381–392
Lichtman JW, Purves D, Yip JW (1980) Innervation of sympathetic neurons in the guinea-pig thoracic chain. J Physiol 298: 285–299
Kamimura K (1987) Effects of stellate ganglion block and cervical epidural block on the carotid blood flow and the cardiac function. Pain Clinic 8: 461–470
Ono K, Kaneko T, Iwatuki N, et al. (1989) The effect of stellate ganglion block and oxygenation on head blood flow. Pain Clinic 10: 211–216
Okuda Y, Kitajima T, Iwanaga H, et al. (1991) Influence of stellate ganglion resection on intracranial pressure. Masui 40: 410–415
McLachlan EM, Jänig W (1983) The cell bodies of origin of sympathetic and sensory axons in some skin and muscle nerves of the cat hindlimb. J Comp Neurol 214: 115–130
Baron R, Jänig W, Kollmann W (1988) Sympathetic and afferent somata projecting in hindlimb nerves and the anatomical organization of the lumbar sympathetic nervous system of the rat. J Comp Neurol 275: 460–468
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Taniguchi, T., Morimoto, M., Taniguchi, Y. et al. Cutaneous distribution of sympathetic postganglionic fibers from stellate ganglion: A retrograde axonal tracing study using wheat germ agglutinin conjugated with horseradish peroxidase. J Anesth 8, 441–449 (1994). https://doi.org/10.1007/BF02514624
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DOI: https://doi.org/10.1007/BF02514624