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Immunolocalizations of human gelatinase (type IV collagenase, MMP-9) and TIMP (tissue inhibitor of metalloproteinases) in normal epidermis and some epidermal tumors

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Abstract

The matrix metalloproteinases (MMPs) MMP-2 and MMP-9 (gelatinases) have been suggested as serving an important role in cleaving the basement membrane structure. Tissue inhibitors of metalloproteinases TIMPs (particularly TIMP-1) are known to inhibit MMPs. Based on this background, we raised monoclonal antibodies against human gelatinase (MMP-9) and human recombinant TIMP (TIMP-1), and immunostained these two components in skin from patients with squamous cell carcinoma (SCC), Bowen's disease (BD) and keratoacanthoma (KA). MMP-9 showed positive staining mainly in the granular layer of normal epidermis. In some cases of SCC and BD, MMP-9 showed positive staining in the dysplastic lesions even in the basal layer. TIMP showed a thorough positivity in normal epidermis. Unstained regions with this antibody were observed in SCC and BD. These results suggest that an altered staining pattern for MMP-9 and TIMP may be closely related to the malignant transformation of SCC and BD.

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Authors and Affiliations

  1. Department of Dermatology, Keio University School of Medicine, Shinanomachi 35, Shinjuku, 160, Tokyo, Japan

    T. Kobayashi, S. Tajima & T. Nishikawa

  2. Department of Pathology, Tokyo Daini National Hospital, Tokyo, Japan

    N. Onoda

  3. Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

    T. Takagi, H. Hori, S. Hattori & Y. Nagai

Authors
  1. T. Kobayashi
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  2. N. Onoda
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  3. T. Takagi
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  4. H. Hori
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  5. S. Hattori
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  6. Y. Nagai
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  7. S. Tajima
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  8. T. Nishikawa
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Kobayashi, T., Onoda, N., Takagi, T. et al. Immunolocalizations of human gelatinase (type IV collagenase, MMP-9) and TIMP (tissue inhibitor of metalloproteinases) in normal epidermis and some epidermal tumors. Arch Dermatol Res 288, 239–244 (1996). https://doi.org/10.1007/BF02530091

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  • DOI: https://doi.org/10.1007/BF02530091

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