Summary
A human cholangiocellular carcinoma cell line, HuCC-T1, was established in vitro from the malignant cells of ascites of a 56-yr-old patient. Histologic findings of the primary liver tumor revealed a moderately differentiated adenocarcinoma. Tumor cells from the ascites have been cultured with RPMI 1640 medium containing 0.2% lactalbumin hydrolysate and the cultured cells grew as monolayers with a population doubling time of 74 h during exponential growth at Passage 25. They had an epithelial-like morphology and were positive for mucine staining. Ultrastructural studies revealed the presence of microvilli on the cell surface and poorly developed organelles in the cytoplasm. The HuCC-T1 cell was tumorigenic in nude mice. The number of chromosomes in HuCC-T1 ranged from 61 to 80. These human cholangiocellular carcinoma cells in serum-free medium secreted several tumor markers, including carbohydrate antigen 19/9, carbohydrate antigen 125, carcinoembryonic antigen, and tissue polypeptide antigen. The carbohydrate antigen 19/9 secretion level of HuCC-T1 cells cultured in PRMI 1640 medium with 1% fetal bovine serum was sixfold higher than that with 0.2% lactalbumin hydrolysate. These findings suggest that HuCC-T1 will provide useful information to clarify the mechanism of tumor marker secretion and tumor cell growth in the human cholangiocellular carcinoma.
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References
Akagi, T., Kimoto, T. Establishment and characterization of a human lung adenocarcinoma cell line. Gann 67:483–492; 1976.
Anthony, P. P. Tumors and tumor-like lesions of the liver and biliary tract. In: MacSween, R. M.; Anthony, P. P.; Scheuer, P. J., eds. Pathology of the liver. New York: Churchill Livingstone Press; 1987:574–688.
Atkinson, B., Ernst, C., Herlyn, M., et al. Gastrointestinal cancer associated antigen in immunoperoxidase assay. Cancer Res. 42:4820–482; 1982.
Bast, R. C., Feeney, M., Lazarus, H., et al. Reactivity of a monoclonal antibody with human ovarian carcinoma. J. Clin. Invest. 68:1331–1337; 1981.
Bast, R. C., Klug, T. L., St. John, E., et al. A radioimmunoassay using a monolonal antibody to monitor the course of epithelial ovarian cancer. N. Engl. J. Med. 309:883–907; 1983.
Björklund, B., Björklund, V. Antigenicity of pooled human malignant and normal tissues by cyto-immunological technique: presence of an insoluble, heat-labile tumor antigen. Int. Arch. Allergy 10:153–184; 1957.
Chen, T. R. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Cell Res. 262:104–255; 1977.
Homma, S., Sujino, H., Yamazaki, K., et al. Human bile duct carcinoma cell line producing abundant mucin in vitro. Acta Hepatol. Jpn. 24:569; 1983.
Ichiki, S., Kuroki, M., Matsunaga, A., et al. Production of immunoreactive calcitonin and some other tumor markers by established human carcinoma cell lines. Gann 77:270–275; 1986.
Iwamura, T., Katsuki, T., Ide, K. Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9. Gann. 78:54–62; 1987.
Klug, T. L., Salzman, S., Quinn, A., et al. Tumorgenicity in athymic mice of the human colon carcinoma cell line SW1116 expressing the tumor-associated antigenic determinant CA19-9. Cancer Res. 44:5212–5218; 1984.
Knuth, A., Gabbert, H., Dippold, W., et al. Biliary adenocarcinoma: characterization of three new human tumor cell lines. J. Hepatol. 1:579–596; 1985.
Koprowski, H.; Steplewski, Z.; Mitchell, K., et al. Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet. 5:957–972; 1979.
Liver Cancer Study Group of Japan. Primary liver cancer in Japan: sixth report. Cancer 60:1400–1411; 1987.
Murakami, T., Yano, H., Maruiwa, M., et al. Establishment and characterization of a human combined hepatocholangio-carcinoma cell line and its heterologous transplantation in nude mice. Hepatology. 7:551–556; 1987.
Nakabayshi, H. M., Taketa, K., Yamane, T., et al. Phenotypical stability of a human hepatoma cell line, HuH-7, in long term culture with chemically defined medium. Gann 75:151–158; 1984.
Okuda, K., Kubo, Y.; Okasaki, N., et al. Clinical aspects of intrahepatic bile duct carcinoma including hilar carcinoma: a study of 57 autopsy-proven cases. Cancer 39:232–246; 1977.
Pastolero, G. C., Wakabayashi, T.; Oka, T., et al. Tissue polypeptide antigen—a marker antigen differentiating cholangiolar tumors from other hepatic tumors. Am. J. Clin. Pathol. 87:168–173; 1987.
Sternberger, L. A. The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Sternberger, L. A., ed. Immunocytochemistry. New York: John Wiley & Sons; 1979:104–169.
Takaki, T. An epithelial cell line (KNS-62) derived from a brain metastasis of bronchial squamous cell carcinoma. J. Cancer Res. Clin. Oncol. 96:27–33; 1980.
Weinbren, K.; Mutum, S. S. Pathological aspects of cholangiocarcinoma. J. Pathol. 139:217–238; 1983.
Yamaguchi, N., Morioka, H.; Ohkura, H., et al. Establishment and characterization of the human cholangiocarcinoma cell line HChol-Yl in a serum-free, chemically defined medium. JNCI 75:29–35; 1985.
Yano, H.; Kojiro, M.; Nakashima, T. A new human hepatocellular carcinoma cell line (KYN-1) with a transformation to adenocarcinoma. In Vitro 22:637–646; 1986.
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Miyagiwa, M., Ichida, T., Tokiwa, T. et al. A new human cholangiocellular carcinoma cell line (HuCC-T1) producing carbohydrate antigen 19/9 in serum-free medium. In Vitro Cell Dev Biol 25, 503–510 (1989). https://doi.org/10.1007/BF02623562
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DOI: https://doi.org/10.1007/BF02623562