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One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin

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Abstract

The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.

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  1. QIAGEN Inc., 9600 DeSoto Avenue, 91311, Chatsworth, CA

    Joanne Crowe, Brigitte Steude Masone & Joachim Ribbe

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  1. Joanne Crowe
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  2. Brigitte Steude Masone
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  3. Joachim Ribbe
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Crowe, J., Masone, B.S. & Ribbe, J. One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin. Mol Biotechnol 4, 247–258 (1995). https://doi.org/10.1007/BF02779018

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