Skip to main content
SpringerLink
Log in

Effect of β3-adrenergic receptor gene polymorphism on body weight change in middle-aged, overweight women

  • Original Article
  • Published:
Environmental Health and Preventive Medicine Aims and scope

Abstract

Objectives

To examine the effects of β3-adrenergic receptor gene polymorphism on body weight change during a weight reduction program for middle-aged, overweight women with careful consideration of their energy intake and expenditure.

Methods

Design: Intervention study of weight reduction for 12 weeks in a community setting. Subjects: Eighty overweight middle-aged women who completed the individualized lifestyle modification program. Measurements: β3-adrenergic receptor gene polymorphism was identified by polymerase chain reaction and consecutive restriction fragment-length polymorphism analysis. Anthropometrical parameters, lifestyle factors, blood lipid and glucose levels, physical activity level and energy intake were measured before and at the end of the program.

Results

The numbers of subjects with the Trp64Trp, Trp64Arg, and Arg64Arg genotypes were 45, 30 and 5, respectively. Baseline characteristics among subjects with the 64Arg allele had significantly smaller decrease in body weight and energy intake than those without the 64Arg allele. The change of other clinical characteristics did not differ between the two groups. After adjusting for the %change of energy intake, the %change of body weight did not differ between the two groups.

Conclusion

The 64Arg allele of the β3-AR gene is not likely to be the factor determining the difficulty in losing body weight in Japanese middle-aged, overweight women. Lifestyle factors, such as the decrease in energy intake, might mask the effect of the 64Arg allele on body weight loss. Specific considerations for the management of energy intake would be needed to promote body weight loss for those with the 64Arg allele.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Emorine LJ, Feve B, Pairault J, Briend-Sutren MM, Nahmias C, Marullo S, et al. The human β3-adrenergic receptor: relationship with atypical receptors. Am J Clin Nutr. 1992; 55:215S-218S.

    PubMed  CAS  Google Scholar 

  2. Krief S, Lonnqvist F, Raimbault S, Baude B, Van Spronsen A, Arner P, et al. Tissue distribution of β3-adrenergic receptor mRNA in man. J Clin Invest. 1993;91:344–349.

    Article  PubMed  CAS  Google Scholar 

  3. Granneman JG. Why do adipocytes make the β3 adrenergic receptor? Cell Signal. 1995;7:9–15.

    Article  PubMed  CAS  Google Scholar 

  4. Strosberg AD, Pietri-Rouxel F. Function and regulation of the β3 adrenoceptor. Trends Pharmacol Sci. 1996;17:373–381.

    PubMed  CAS  Google Scholar 

  5. Granneman JG, Burnazi M, Zhu Z, Schwamb LA. White adipose tissue contributes to UCP1-independent thermogenesis. Am J Physiol. 2003;285:E1230-E1236.

    CAS  Google Scholar 

  6. Walston J, Andersen RE, Seibert M, Hilfiker H, Beamer B, Blumenthal J, et al. Arg64 β3-adrenergic variant and the components of energy expenditure. Obes Res. 2003;11:509–511.

    Article  PubMed  CAS  Google Scholar 

  7. Walston J, Silver K, Bogardus C, Knowler WC, Celi FS, Austin S, et al. Time of onset of non-insulin-dependent diabetes mellitus and genetic variation in the β3-adrenergic-receptor gene. N Engl J Med. 1995;333:343–347.

    Article  PubMed  CAS  Google Scholar 

  8. Clement K, Vaisse C, Manning BS, Basdevant A, Guy Grand B, Ruiz J, et al. Genetic variation in the β3-adrenergic receptor and an increased capacity to gain weight in patients with morbid obesity. N Engl J Med. 1995;333:352–354.

    Article  PubMed  CAS  Google Scholar 

  9. Fujisawa T, Ikegami H, Kawaguchi Y, Ogihara T. Meta-analysis of the association of Trp64arg polymorphism of β3-adrenergic receptor gene with body mass index. J Clin Endocrinol Metab. 1998;83:2441–2444.

    Article  PubMed  CAS  Google Scholar 

  10. Kim-Motoyama H, Yasuda K, Yamaguchi T, Yamada N, Katkura T, Shuldiner AR, et al. A mutation of the β3-adrenergic receptor is associated with visceral obesity but decreased serum triglyceride. Diabetologia. 1997;40:469–472.

    Article  PubMed  CAS  Google Scholar 

  11. Mitchell BD, Cole SA, Comuzzie AG, Almasy L, Blangero J, MacCluer JW, et al. A quantitative trait locus influencing BMI maps to the region of the β3 adrenergic receptor. Diabetes. 1999;48:1863–1867.

    Article  PubMed  CAS  Google Scholar 

  12. Widen E, Lehto M, Kanninen T, Walston J, Schuldiner AR, Groop LC. Association of a polymorphism in the β3-adrenergic receptor gene with features of the insulin resistance syndrome in Finns. N Engl J Med. 1995;333:348–351.

    Article  PubMed  CAS  Google Scholar 

  13. Mauriege P, Bouchard C. Trp64Arg mutation in β3-adrenoceptor gene of doubtful significance for obesity and insulin resistance. Lancet. 1996;348:698–699.

    Article  PubMed  CAS  Google Scholar 

  14. Knowler WC. Association of Trp64Arg mutation of the β3-adrenergic receptor gene with NIDDM. Diabetologia. 1996;39:1411.

    PubMed  CAS  Google Scholar 

  15. Odawara M, Sasaki K, Yamashita K. β3-adrenergic receptor gene variant and Japanese NIDDM: a pitfall in meta-analysis. Lancet. 1996;348:896–897.

    Article  PubMed  CAS  Google Scholar 

  16. Buettner R, Schsffler A, Arndt H, Rogler G, Nusser J, Zietz B, et al. The Trp64Arg polymorphism of the β3-adrenergic receptor gene is not associated with obesity or type 2 diabetes mellitus in a large population-based Caucasian cohort. J Clin Endocrinol Metab. 1998;83:2892–2897.

    Article  PubMed  CAS  Google Scholar 

  17. Matsushita Y, Yokoyama T, Yoshiike N, Matsumura Y, Date C, Kawahara K, et al. The Trp64Arg polymorphism of the β3-adrenergic receptor gene is not associated with body weight or body mass index in Japanese: A longitudinal analysis. J Clin endocrinol Metab. 2003;88:5914–5920.

    Article  PubMed  CAS  Google Scholar 

  18. Matsushita H, Kurabayashi T, Tomita M, Kato N, Tanaka K. Effects of uncoupling protein 1 and β3-adrenergic receptor gene polymorphism on body size and serum lipid concentrations in Japanese women. Maturitas. 2003;45:39–45.

    Article  PubMed  CAS  Google Scholar 

  19. Garcia-Rubi E, Calles-Escandon J. Insulin resistance and type 2 diabetes mellitus: its relationship with the β3-adrenergic receptor. Arch Med Res. 1999;30:459–464.

    Article  PubMed  CAS  Google Scholar 

  20. Lee JS. β3-adrenergic receptor gene variant. Nippon Rinsho. 2001;59:785–789. (Article in Japanese)

    PubMed  Google Scholar 

  21. Marti A, Corbalan MS, Martinez-Gonzalez MA, Martinez JA. Trp64Arg polymorphism of the β3-adrenergic receptor gene and obesity risk: effect modification by a sedentary lifestyle. Diabetes Obes Metab. 2002;4:428–430.

    Article  PubMed  CAS  Google Scholar 

  22. Oksanen L, Mustajoki P, Kaprio J, Kainulainen K, Janne O, Peltonen L, et al. Polymorphism of the β3-adrenergic receptor gene in morbid obesity Int J Obes Relat Metab Disord. 1996;20:1055–1061.

    PubMed  CAS  Google Scholar 

  23. Fumeron F, Durack-Brown I, Betoulle D, Cassard-Doulcier AM, Tuzet S, Bouillaud F, et al. Polymorphisms of uncoupling protein (UCP) and β3 adrennoreceptor genes in obese people submitted to a low calorie diet. Int J Obes Relat Metab Disord. 1996;20:1051–1054.

    PubMed  CAS  Google Scholar 

  24. Tchernof A, Starling RD, Tuener A, Shuldiner AR, Walston JD, Silver K, et al. Impaired capacity to loss visceral adipose tissue during weight reduction in obese postmenopausal women with the Trp64Arg β3-adrenoceptor gene variant. Diabetes. 2000;49:1709–1713.

    Article  PubMed  CAS  Google Scholar 

  25. Benecke H, Topak H, von zur Muhlen A, Schuppert F. A study on the genetics of obesity: influence of polymorphisms of the β3-adrenergic receptor and insulin receptor substrate 1 in relation to weight loss, waist to hip ratio and frequencies of common cardiovascular risk factors. Exp Clin Endocrinol Diabetes. 2000;108:86–92.

    Article  PubMed  CAS  Google Scholar 

  26. Rawson ES, Nolan A, Silver K, Shuldiner AR, Poehlman ET. No effects of the Trp64Arg β3-adrenoceptor gene variant on weight loss, body composition, or energy expenditure in obese, Caucasian postmenopausal women. Metabolism. 2002; 51:801–805.

    Article  PubMed  CAS  Google Scholar 

  27. Yoshida T, Sakane N, Umekawa T, Sakai M, Takahashi T, Kondo M. Mutation of beta sub 3-adrenergic-receptor gene and response to treatment of obesity. Lancet. 1995;346:1433–1434.

    Article  PubMed  CAS  Google Scholar 

  28. Sakane N, Yoshida T, Umekawa T, Kogure A, Takakura Y, Kondo M. Effects of Trp64Arg mutation in β3-adrenergic receptor gene on weight loss, body fat distribution, glycemic control, and insulin resistance in obese type 2 diabetic patients. Diabetes Care. 1997;20:1887–1890.

    Article  PubMed  CAS  Google Scholar 

  29. Shiwaku K, Nogi A, Anuuard E, Kitajima K, Enkhmaa B, Shimono K, et al. Difficulty in losing weight by behavioral intervention for women with Trp64Arg polymorphism of the β3-adrenergic receptor gene. Int J Obes Relat Metab Disord. 2003;27:1028–1036.

    Article  PubMed  CAS  Google Scholar 

  30. Kim OY, Cho EY, Park HY, Jang Y, Lee JH. Additive effect of the mutations in the β3-adrenoceptor gene and UCP3 gene promoter on body fat distribution and glycemic control after weight reduction in overweight subjects with CAD or metabolic syndrome. Int J Obes Relat Metab Disord. 2004; 28:434–441.

    Article  PubMed  CAS  Google Scholar 

  31. Kadowaki H, Yasuda K, Iwamoto K, Otabe S, Shimokawa K, Silver K, et al. A mutation in the β3-adrenergic receptor gene is associated with obesity and hyperinsulinemia in Japanese subjects. Biochem Biophys Res Commun. 1995;145:555–560.

    Article  Google Scholar 

  32. Nagamine S, Suzuki S. Anthropometry and body composition of Japanese young men and women. Hum Biol. 1964;36:8–15.

    PubMed  CAS  Google Scholar 

  33. Brozek J, Grande F, Anderson JT, Keys A. Densitometric analysis of body composition: revision of some quantitative assumption. Ann NY Acad Sci. 1963;110:113–140.

    Article  PubMed  CAS  Google Scholar 

  34. Resources Council, Science and Technology Agency, Japan. Standard Tables of Food Composition in Japan, 5th revised ed., Tokyo, 2000.

  35. Friedewald WT, Levy RI, Fredrick DS. Estimation of the concentration of low density lipoprotein in plasma without use of a preparative ultracentrifuge. Clin Chem. 1972;18:449–456.

    Google Scholar 

  36. Allison DB, Heo M, Faith MS, Pietrobelli A. Meta-analysis of the association of the Trp64Arg polymorphism in the β3 adrenergic receptor with body mass index. Int J Obes Relat Metab Disord. 1998;22:559–566.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Health Promotion Sciences, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan

    Jung Su Lee

  2. Department of Food Sciences & Nutrition, Kyoritsu Women’s University, Tokyo, Japan

    Kiyoshi Kawakubo & Shuji Inoue

  3. Department of Biomedical Ethics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Akira Akabayashi

Authors
  1. Jung Su Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kiyoshi Kawakubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuji Inoue
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akira Akabayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jung Su Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, J.S., Kawakubo, K., Inoue, S. et al. Effect of β3-adrenergic receptor gene polymorphism on body weight change in middle-aged, overweight women. Environ Health Prev Med 11, 69–74 (2006). https://doi.org/10.1007/BF02898145

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02898145

Key words

Search

Navigation