Abstract
The nucleotide sequence was determined for Czech potato mop-top virus (PMTV) isolate Korneta-Nemilkov, found in the potato field situated in South Bohemia. The nucleotide and amino acid sequences were compared with other PMTV isolates available in databases. The sequence identity was always >99 % when Czech isolate RNA 2 and RNA 3 sequences were compared with each of the 3 Danish isolates and with Sw isolate, and slightly lower when compared to Scottish isolates. Similarity of deduced proteins was 100 % for 5 out of 6 proteins used in comparison of Czech isolate with Danish isolate 54-15. The only difference between 2 isolates was found in coat protein (CP) gene. Interestingly, the CP of the Czech isolate seems to be 100 % identical to the one of Sw, while many changes were found in the region encoding TGBp2, TGBp3 and cysteine-rich protein (CRP) for these 2 isolates. The lowest similarity scores were found when comparing the Czech isolate CRP with CRP of Scottish isolates.
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Abbreviations
- CP:
-
coat protein
- CRP:
-
cysteine-rich protein
- PMTV:
-
potato mop-top virus
- ORF:
-
open reading frame(s)
- TGB:
-
triple gene block
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This research was supported by grant no. 522/04/1329 of theGrant Agency of the Czech Republic and by grant no. 1M06030 of theMinistry of Education, Youth and Sports of the Czech Republic.
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Čeřovská, N., Pečenková, T., Filigarová, M. et al. Sequence analysis of the Czech Potato mop-top virus (PMTV) isolate Korneta-Nemilkov. Folia Microbiol 52, 61–64 (2007). https://doi.org/10.1007/BF02932139
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DOI: https://doi.org/10.1007/BF02932139