Skip to main content
SpringerLink
Log in

Characterization and detection ofCucumber green mottle mosaic virus in Greece

  • Research Papers
  • Published:
Phytoparasitica Aims and scope Submit manuscript

Abstract

The host range specificity of Greek isolates ofCucumber green mottle mosaic virus (CGMMV) was determined and the ability of the virus to retain its infectivity in naturally contaminated soil, after storage at 4°C for at least 10 months, was established. An immunocapture RT-PCR protocol was developed for the detection of the virus and proved to be 105 times more sensitive than DAS-ELISA and 102 times more sensitive than F(ab’)2-ELISA using F(ab’)2 fragments that were prepared from an antiserum, which was raised against a local virus isolate. In order to clarify dissimilarities that were revelaed between the coat protein (CP) genes of the Greek isolates after restriction mapping of their respective PCR products, the CP genes of three isolates were sequenced and found to be very similar but not identical to the CP gene of CGMMV-SH.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ainsworth, G.C. (1935) Mosaic disease of cucumber.Ann. Appl. Biol. 22:55–67.

    Article  Google Scholar 

  2. Antignus, Y., Pearlsman, M., Ben-Yoseph, R. and Cohen, S. (1990) Occurrence of a variant of cucumber green mottle mosaic virus in Israel.Phytoparasitica 18:50–56.

    Google Scholar 

  3. Antignus, Y., Wang, Y., Pearlsman, M., Lachman, O., Lavi, N. and Gal-On, A. (2001) Biological and molecular characterization of a new cucurbit-infecting tobamovirus.Phytopathology 91:565–557.

    Article  PubMed  CAS  Google Scholar 

  4. Avgelis, A.D. and Vovlas, C. (1986) Occurrence of Cucumber green mottle mosaic virus in the island of Crete (Greece).Phytopathol. Mediterr. 25:166–168.

    Google Scholar 

  5. Barbara, D.J. and Clark, M.F. (1982) A simple indirect ELISA using F(ab’)2 fragments of immunoglobulin.J. Gen. Virol. 58:315–322.

    Article  PubMed  CAS  Google Scholar 

  6. Bem, F.P. and Vassilakos, N. (2000) Occurrence of the disease “Internal deterioration of watermelon fruit” in watermelon cultivation in Greece.Phytopathol. Mediterr. 39:325 (abstract).

    Google Scholar 

  7. Celix, A., Luis-Arteaga, M. and Rodriguez-Cerezo, E. (1996) First report of Cucumber green mottle mosaic Tobamovirus infecting greenhouse-grown cucumber in Spain.Plant Dis. 80:1303.

    Google Scholar 

  8. Francki, R.I.B., Hu, J. and Palukaitis, P. (1986) Taxonomy of cucurbit-infecting Tobamovirus as determined by serological and molecular hybridization analyses.Intervirology 26:156–163.

    Article  PubMed  CAS  Google Scholar 

  9. Ryu, K.H., Min, B.E., Choi, G.S., Choi, S.H., Kwon, S.B., Noh, G.M.et al. (2000) Zucchini green mottle mosaic virus is a new tobamovirus: comparison of its coat protein gene with that of kyuri green mottle mosaic virus.Arch. Virol. 145:2325–2333.

    Article  PubMed  CAS  Google Scholar 

  10. Tan, S.-H., Nishiguchi, M., Murata, M. and Motoyoshi, F. (2000) The genome structure of kyuri green mottle mosaic tobamovirus and its comparison with that of cucumber green mottle mosaic tobamovirus.Arch. Virol. 145:1067–1079.

    Article  PubMed  CAS  Google Scholar 

  11. Thomson, J.D., Higgins, D.G. and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic Acids Res. 22:4673–4680.

    Article  Google Scholar 

  12. Tung, J.S. and Knight, C.A. (1972) The coat protein subunits of cucumber viruses 3 and 4 and a comparison of methods for determining their weights.Virology 48:574–581.

    Article  PubMed  CAS  Google Scholar 

  13. Ugaki, M., Tomiyama, M., Kakutani, T., Hidaka, S., Kiguchi, T., Nagata, R.et al. (1991) The complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA.J. Gen. Virol. 72:1487–1495.

    Article  PubMed  CAS  Google Scholar 

  14. Vani, S. and Varma, A. (1993) Properties of cucumber green mottle mosaic virus isolated from water of river Jamuna.Indian Phytopathol. 46:118–122.

    Google Scholar 

  15. Yoon, J.Y., Min, B.E., Choi, J.K. and Ryu, K.H. (2002) Genome structure and production of biologically active in vitro transcripts of cucurbits-infecting Zucchini green mottle mosaic virus.Phytopathology 92:156–163.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Virology, Benaki Phytopathological Institute, GR-145 61, Kifissia, Athens, Greece

    C. Varveri, N. Vassilakos & F. Bem

Authors
  1. C. Varveri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Vassilakos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Bem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Varveri.

Additional information

http://www.phytoparasitica.org posting Aug. 27, 2002.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Varveri, C., Vassilakos, N. & Bem, F. Characterization and detection ofCucumber green mottle mosaic virus in Greece. Phytoparasitica 30, 493–501 (2002). https://doi.org/10.1007/BF02979754

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02979754

Key words

Search

Navigation