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01.09.2010 | Original Research Paper

Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats

verfasst von: Fabricia Petronilho, Bruna de Souza, Francieli Vuolo, César A. F. Benetton, Emilio L. Streck, Rafael Roesler, Gilberto Schwartsmann, Felipe Dal-Pizzol

Erschienen in: Inflammation Research | Ausgabe 9/2010

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Abstract

Objective

We report the effects of the gastrin-releasing peptide (GRP) receptor antagonist RC-3095 in an acute inflammation model induced by carrageenan.

Methods

Male Wistar rats received saline or saline containing 2% λ-carrageenan into the pleural cavity, with some also receiving RC-3095 3 mg/kg subcutaneously, immediately after surgery. Four hours later, the rats were killed and pleural exudate was obtained for evaluation of total cell count, lactate dehydrogenase activity, total protein, cytokines analysis and nitrite/nitrate concentrations; myeloperoxidase (MPO) activity and oxidative stress were evaluated in the lung.

Results

RC-3095 exhibited pronounced anti-inflammatory actions by inhibition of leukocyte influx and blockade of MPO, nitrite/nitrate and cytokine levels. Moreover, the results showed that RC-3095 elicits action against oxidative damage in lipids and proteins, as well as increasing cell viability.

Conclusion

The present findings suggest that GRP plays a role in acute inflammation that can be related with the reduction of oxidative damage and that it could be effective in therapeutic applications.

Lentsch AB, Ward PA. Regulation of experimental lung inflammation. Respir Physiol. 2001;128:17–21.CrossRefPubMed

Vinegar R, Truax JF, Selph JL, Voelker FA. Pathway of onset, development, and decay of carrageenan pleurisy in the rat. Fed Proc. 1982;41:2588–95.PubMed

Salvemini D, Wang ZQ, Wyatt P, Bourdon DM, Marino MH, Manning PT, et al. Nitric oxide: a key mediator in the early and late phase of carrageenan-induced rat paw inflammation. Br J Pharmacol. 1996;118:829–37.PubMed

Salvemini D, Masferrer JL. Interactions of nitric oxide witch cyclooxygenase: in vitro, ex vivo, and in vivo studies. Methods Enzymol. 1996;269:12–25.CrossRefPubMed

Fialkow L, Wang Y, Downey GP. Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function. Free Radic Biol Med. 2007;42:153–64.CrossRefPubMed

Guo RF, Ward PA. Role of oxidants in lung injury during sepsis. Antioxid Redox Signal. 2007;9:02–1991.

Medina S, Del Rio M, Victor VM, Hernánz A, De la Fuente M. Changes with ageing in the modulation of murine lymphocyte chemotaxis by CCK-8S, GRP and NPY. Mech Ageing Dev. 1998;102:249–61.CrossRefPubMed

Medina S, Rio MD, De la Cuadra B, Fuente MD. Age-related changes in the modulatory action of gastrin-releasing peptide, neuropeptide Y and sulfated cholecystokinin octapeptide in the proliferation of murine lymphocytes. Neuropeptides. 1999;33:173–9.CrossRefPubMed

Genton L, Kudsk KA. Interactions between the enteric nervous system and the immune system: role of neuropeptides and nutrition. Am J Surg. 2003;186:253–8.CrossRefPubMed

10.

Sunday ME, Yoder BA, Cuttitta F, Haley KJ, Emanuel RL. Bombesin like peptide mediates lung injury in a baboon model of bronchopulmonary dysplasia. J Clin Invest. 1998;102:584–94.CrossRefPubMed

11.

Subramaniam M, Sugyiama K, Coy DH, Kong Y, Miller YE, Weller PF, et al. Bombesin-like peptides and mast cell responses: relevance to bronchopulmonary dysplasia? Am J Respir Crit Care Med. 2003;168:601–11.CrossRefPubMed

12.

Sunday ME. Neuropeptides and lung development. In: McDonald JA, editor. Lung growth and development. New York: Marcel Dekker; 1997. p. 401–94.

13.

Grimsholm O, Rantapaa-Dahlqvist S, Forsgren S. Levels of gastrin-releasing peptide and substance P in synovial fluid and serum correlate with levels of cytokines in rheumatoid arthritis. Arthritis Res Ther. 2005;7:416–26.CrossRef

14.

Petronilho F, Roesler R, Schwartsmann G, Dal-Pizzol F. Gastrin-releasing peptide receptor as a molecular target for inflammatory diseases. Inflamm Allergy Drug Targets. 2007;6:197–200.CrossRefPubMed

15.

Dal-Pizzol F, Di Leone LP, Ritter C, Martins MR, Reinke A, Pens Gelain D, et al. Gastrin-releasing peptide receptor antagonist effects on an animal model of sepsis. Am J Respir Crit Care Med. 2006;173:84–90.CrossRefPubMed

16.

Oliveira PG, Brenol CV, Edelweiss MI, Brenol JC, Petronilho F, Roesler R, et al. Effects of an antagonist of the bombesin/gastrin-releasing peptide receptor on complete Freund’s adjuvant-induced arthritis in rats. Peptides. 2008;29:1726–31.CrossRefPubMed

17.

Radulovic S, Cai R-Z, Serfozo P, Groot K, Redding TW, Pinski J, et al. Biological effects and receptor binding affinities of new pseudononapeptide bombesin/GRP receptor antagonists with N-terminal d-Trp or d-Tpi. Int J Pept Protein Res. 1991;38:593–600.PubMedCrossRef

18.

Vinegar R, Traux JF, Selph JL. Some quantitative temporal characteristics of carrageenan-induced pleurisy in the rat. Proc Soc Exp Biol Med. 1943;143:711–4.

19.

Lowry OH, Rosebrough AL, Randal RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265–75.PubMed

20.

Green IC, Wagner DA, Glowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and [15N] nitrate in biological fluids. Anal Biochem. 1982;126:131–8.CrossRefPubMed

21.

De Young LM, Kheifets JB, Ballaron SJ, Young JM. Edema and cell infiltration in the phorbol ester-treated mouse ear are temporally separate and can be differentially modulated by pharmacologic agents. Agents Actions. 1989;26:335–41.CrossRefPubMed

22.

Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990;186:421–31.CrossRefPubMed

23.

Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 1990;186:464–78.CrossRefPubMed

24.

Riddles PW, Blakeley RL, Zerner B. Reassessment of Ellman’s reagent. Methods Enzymol. 1983;91:49–60.CrossRefPubMed

25.

Kroegel C, Antony VB. Immunobiology of pleural inflammation: potential implications for pathogenesis, diagnosis and therapy. Eur Respir J. 1997;10:2411–8.CrossRefPubMed

26.

Segura RM. Useful clinical biological markers in diagnosis of pleural effusions in children. Paediatr Respire Rev. 2004;5:205–12.CrossRef

27.

Antony VB, Godbey SW, Kunkel SL, Hott JW, Hartman DL, Burdick MD, et al. Recruitment of inflammatory cells to the pleura space: chemotactic cytokines IL-8, and monocyte chemotactic peptide-1 in human pleural fluids. J Immunol. 1993;151:7216–23.PubMed

28.

Romano M, Sironi M, Toniatti C, Polentarutti N, Fruscella P, Ghezzi P, et al. Role of IL-6 and its soluble receptor in induction of chemokines and leukocyte recruitment. Immunity. 1997;6:315–25.CrossRefPubMed

29.

Alonzi T, Fattori E, Cappelletti M, Ciliberto G, Poli V. Impaired stat3 activation following localized inflammatory stimulus in IL-6 deficient mice. Cytokine. 1998;10:13–8.CrossRefPubMed

30.

Utsunomiya I, Nagai S, Oh-ishi S. Sequential appearance of IL-1 and IL-6 activities in rat carrageenan-induced pleurisy. J Immunol. 1991;147:1803–9.PubMed

31.

Oh-Ishi S, Hayashi I, Hayashi M, Yamaki K, Utsunomiya I. Pharmacological demonstration of inflammatory mediators using experimental inflammatory models: rat pleurisy induced by carrageenin and phorbol myristate acetate. Dermatologica. 1989;179:68–71.CrossRefPubMed

32.

Tracey WR, Nakane M, Kuk J, Budzik G, Klinghofer V, Harris R. The nitric oxide synthase inhibitor, l-NG-monomethylarginine, reduces carrageenan-induced pleurisy in the rat. J Pharmacol Exp Ther. 1995;273:1295–9.PubMed

33.

Wei XQ, Charles IG, Smith A, Ure J, Feng GJ, Huang FP, et al. Altered immune responses in mice lacking inducible nitric oxide synthase. Nature. 1995;375:408–11.CrossRefPubMed

34.

Cuzzocrea S, Zingarelli B, Costantino G, Caputi AP. Beneficial effects of Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP), a superoxide dismutase mimetic, in carrageenan-induced pleurisy. Free Radic Biol Med. 1999;26:25–33.CrossRefPubMed

35.

Cuzzocrea S, Zingarelli B, Hake P, Salzman AL, Szabó C. Antiinflammatory effects of mercaptoethylguanidine, a combined inhibitor of nitric oxide synthase and peroxynitrite scavenger, in carrageenan-induced models of inflammation. Free Radic Biol Med. 1998;24:450–9.CrossRefPubMed

36.

Szabo C, Wu CC, Gross SS, Thiemermann C, Vane JR. Interleukin-1 contributes to the induction of nitric oxide synthase by endotoxin in vivo. Eur J Pharmacol. 1993;250:157–60.CrossRefPubMed

37.

Jin CY, Moon DO, Lee KJ, Kim MO, Lee JD, Choi YH, et al. Piceatannol attenuates lipopolysaccharide-induced NF-kappaB activation and NFkappaB-related proinflammatory mediators in BV2 microglia. Pharmacol Res. 2006;54:461–7.CrossRefPubMed

38.

Mariotto S, Esposito E, Di Paola R, Ciampa A, Mazzon E, de Prati AC, et al. Protective effect of Arbutus unedo aqueous extract in carrageenan-induced lung inflammation in mice. Pharmacol Res. 2008;57:110–24.CrossRefPubMed

39.

Yasui K, Baba A. Therapeutic potential of superoxide dismutase (SOD) for resolution of inflammation. Inflamm Res. 2006;55:359–63.CrossRefPubMed

40.

Cuzzocrea S, McDonald MC, Filipe HM, Costantino G, Mazzon E, Santagati S, et al. Effects of tempol, a membrane-permeable radical scavenger, in a rodent model of carrageenan-induced pleurisy. Eur J Pharmacol. 2000;390:209–22.CrossRefPubMed

41.

Nardi GM, Siqueira Junior JM, Delle Monache F, Pizzolatti MG, Ckless K, Ribeiro-do-Valle RM. Antioxidant and anti-inflammatory effects of products from Croton celtidifolius Bailon on carrageenan-induced pleurisy in rats. Phytomedicine. 2007;14:115–22.CrossRefPubMed

42.

Bhattacharyya S, Dudeja PK, Tobacman JK. Carrageenan-induced NFkappaB activation depends on distinct pathways mediated by reactive oxygen species and Hsp27 or by Bcl10. Biochim Biophys Acta. 2008;1780:973–82.PubMed

43.

Menegazzi M, Di Paola R, Mazzon E, Genovese T, Crisafulli C, Dal Bosco M, et al. Glycyrrhizin attenuates the development of carrageenan-induced lung injury in mice. Pharmacol Res. 2008;58:22–31.CrossRefPubMed

44.

Aksenov MY, Markesbery WR. Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer’s disease. Neurosci Lett. 2001;302:141–5.CrossRefPubMed

45.

Kirkham P, Rahman I. Oxidative stress in asthma and COPD: antioxidants as a therapeutic strategy. Pharmacol Ther. 2006;111:476–94.CrossRefPubMed

46.

Fröde-Saleh TS, Calixto JB. Synergistic antiinflammatory effect of NF-kappaB inhibitors and steroidal or non steroidal antiinflammatory drugs in the pleural inflammation induced by carrageenan in mice. Inflamm Res. 2000;49:330–7.CrossRefPubMed

47.

Levine L, Lucci JA, Pazdrak B, Cheng JZ, Guo YS, Townsend CM Jr, et al. Bombesin stimulates nuclear factor κB activation and expression of proangiogenic factors in prostate cancer cells. Cancer Res. 2003;63:3495–502.PubMed

Titel: Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats
verfasst von: Fabricia Petronilho
Bruna de Souza
Francieli Vuolo
César A. F. Benetton
Emilio L. Streck
Rafael Roesler
Gilberto Schwartsmann
Felipe Dal-Pizzol
Publikationsdatum: 01.09.2010
Verlag: SP Birkhäuser Verlag Basel
Erschienen in: Inflammation Research / Ausgabe 9/2010
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-010-0190-8

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Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats

Abstract

Objective

Methods

Results

Conclusion

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Abstract

Objective

Methods

Results

Conclusion

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