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Inflammation Research

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01.02.2011 | Original Research Paper

Polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells

verfasst von: C. B. Huang, Y. Altimova, S. Strange, J. L. Ebersole

Erschienen in: Inflammation Research | Ausgabe 2/2011

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Abstract

Objective

To investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (DCs), at the mucosal surface.

Materials and methods

The cytokine/chemokine profiles of human macrophages and DCs in response to polybacterial challenges were investigated.

Results

Oral Gram-negative bacteria elicited significantly greater IL-8 levels from macrophages, compared to Gram-positive bacteria. Gram-positive bacteria did not show synergism in inducing this chemokine from macrophages. In contrast, pairs of oral Gram-negative bacteria elicited synergistic production of IL-8 by macrophages. Similar results were not observed with TNFα, which only appeared additive with the polybacterial challenge. Selected Gram-negative bacterial pairs synergized in IL-6 production by immature DCs. In mature DCs (mDCs), a Porphyromonas gingivalis/Fusobacterium nucleatum and Porphyromonas intermedia/F. nucleatum polybacterial challenge resulted in significant synergism for IL-6 and TNFα levels. However, only the Pi/Fn combination synergized for IL-12 production and there appeared to be no polybacterial effect on IL-10 production by the mDCs.

Conclusions

These results indicate that a polybacterial challenge of cells linking innate and adaptive immune responses results in varied response profiles that are dependent upon the characteristics of the microorganisms that are components of the polybacterial complex.

Tatakis DN, Kumar PS. Etiology and pathogenesis of periodontal diseases. Dent Clin North Am. 2005;49:491–516 (V).CrossRefPubMed

Kinane DF, Bartold PM. Clinical relevance of the host responses of periodontitis. Periodontol 2000. 2007;43:278–93.CrossRefPubMed

Vaughan AT, Gorringe A, Davenport V, Williams NA, Heyderman RS. Absence of mucosal immunity in the human upper respiratory tract to the commensal bacteria Neisseria lactamica but not pathogenic Neisseria meningitidis during the peak age of nasopharyngeal carriage. J Immunol. 2009;182:2231–40.CrossRefPubMed

Cario E. Innate immune signalling at intestinal mucosal surfaces: a fine line between host protection and destruction. Curr Opin Gastroenterol. 2008;24:725–32.CrossRefPubMed

Edelman SM, Kasper DL. Symbiotic commensal bacteria direct maturation of the host immune system. Curr Opin Gastroenterol. 2008;24:720–4.CrossRefPubMed

Boirivant M, Amendola A, Butera A. Intestinal microflora and immunoregulation. Mucosal Immunol. 2008;1(Suppl 1):S47–9.CrossRefPubMed

Rescigno M, Lopatin U, Chieppa M. Interactions among dendritic cells, macrophages, and epithelial cells in the gut: implications for immune tolerance. Curr Opin Immunol. 2008;20:669–75.CrossRefPubMed

Paster BJ, Olsen I, Aas JA, Dewhirst FE. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 2000. 2006;42:80–7.CrossRefPubMed

Ledder RG, Gilbert P, Huws SA, Aarons L, Ashley MP, Hull PS, et al. Molecular analysis of the subgingival microbiota in health and disease. Appl Environ Microbiol. 2007;73:516–23.CrossRefPubMed

10.

Kumar PS, Griffen AL, Moeschberger ML, Leys EJ. Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis. J Clin Microbiol. 2005;43:3944–55.CrossRefPubMed

11.

Dye BA, Choudhary K, Shea S, Papapanou PN. Serum antibodies to periodontal pathogens and markers of systemic inflammation. J Clin Periodontol. 2005;32:1189–99.CrossRefPubMed

12.

Ebersole JL. Humoral immune responses in gingival crevice fluid: local and systemic implications. Periodontol 2000. 2003;31:135–66.CrossRefPubMed

13.

Kocsis J, Veres A, Vatay A, Duba J, Karadi I, Fust G, et al. Antibodies against the human heat shock protein hsp70 in patients with severe coronary artery disease. Immunol Invest. 2002;31:219–31.CrossRefPubMed

14.

Mooney J, Hodge PJ, Kinane DF. Humoral immune response in early-onset periodontitis: influence of smoking. J Periodontal Res. 2001;36:227–32.CrossRefPubMed

15.

Kinane DF, Lappin DF. Clinical, pathological and immunological aspects of periodontal disease. Acta Odontol Scand. 2001;59:154–60.CrossRefPubMed

16.

Ebersole JL, Cappelli D, Holt SC. Periodontal diseases: to protect or not to protect is the question? Acta Odontol Scand. 2001;59:161–6.CrossRefPubMed

17.

Furuichi Y, Shimotsu A, Ito H, Namariyama Y, Yotsumoto Y, Hino Y, et al. Associations of periodontal status with general health conditions and serum antibody titers for Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. J Periodontol. 2003;74:1491–7.CrossRefPubMed

18.

Kinane DF, Podmore M, Murray MC, Hodge PJ, Ebersole J. Etiopathogenesis of periodontitis in children and adolescents. Periodontol. 2000. 2001;26:54–91.CrossRefPubMed

19.

Darby IB, Mooney J, Kinane DF. Changes in subgingival microflora and humoral immune response following periodontal therapy. J Clin Periodontol. 2001;28:796–805.CrossRefPubMed

20.

Papapanou PN, Neiderud AM, Papadimitriou A, Sandros J, Dahlen G. “Checkerboard” assessments of periodontal microbiota and serum antibody responses: a case–control study. J Periodontol. 2000;71:885–97.CrossRefPubMed

21.

Ebersole JL, Cappelli D, Steffen MJ, Willmann DE, O’Dell DS. Host response assessment in recurring periodontitis. J Clin Periodontol. 1996;23:258–62.CrossRefPubMed

22.

Hafstrom CA, Wikstrom MB, Renvert SN, Dahlen GG. Effect of treatment on some periodontopathogens and their antibody levels in periodontal abscesses. J Periodontol. 1994;65:1022–8.PubMed

23.

Taubman MA, Haffajee AD, Socransky SS, Smith DJ, Ebersole JL. Longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases. J Periodontal Res. 1992;27:511–21.CrossRefPubMed

24.

Ebersole JL, Taubman MA, Smith DJ, Frey DE, Haffajee AD, Socransky SS. Human serum antibody responses to oral microorganisms. IV. Correlation with homologous infection. Oral Microbiol Immunol. 1987;2:53–9.CrossRefPubMed

25.

Ting JP, Duncan JA, Lei Y. How the noninflammasome NLRs function in the innate immune system. Science. 2010;327:286–90.CrossRefPubMed

26.

Rehwinkel J, Reis e Sousa C. RIGorous detection: exposing virus through RNA sensing. Science. 2010;327:284–6.CrossRefPubMed

27.

Mueller KL. Innate immunity. Recognizing the first responders. Introduction. Science. 2010;327:283.CrossRefPubMed

28.

Iwasaki A, Medzhitov R. Regulation of adaptive immunity by the innate immune system. Science. 2010;327:291–5.CrossRefPubMed

29.

Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K. Development of monocytes, macrophages, and dendritic cells. Science. 2010;327:656–61.CrossRefPubMed

30.

Cutler CW, Jotwani R. Dendritic cells at the oral mucosal interface. J Dent Res. 2006;85:678–89.CrossRefPubMed

31.

Mascarell L, Lombardi V, Louise A, Saint-Lu N, Chabre H, Moussu H, et al. Oral dendritic cells mediate antigen-specific tolerance by stimulating TH1 and regulatory CD4+ T cells. J Allergy Clin Immunol. 2008;122:603–9 (e5).CrossRefPubMed

32.

Conti L, Cardone M, Varano B, Puddu P, Belardelli F, Gessani S. Role of the cytokine environment and cytokine receptor expression on the generation of functionally distinct dendritic cells from human monocytes. Eur J Immunol. 2008;38:750–62.CrossRefPubMed

33.

Blander JM. Phagocytosis and antigen presentation: a partnership initiated by Toll-like receptors. Ann Rheum Dis. 2008;67 (Suppl 3):iii44–9.

34.

Allenspach EJ, Lemos MP, Porrett PM, Turka LA, Laufer TM. Migratory and lymphoid-resident dendritic cells cooperate to efficiently prime naive CD4 T cells. Immunity. 2008;29:795–806.CrossRefPubMed

35.

Lukic A, Vasilijic S, Majstorovic I, Vucevic D, Mojsilovic S, Gazivoda D, et al. Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry. Int Endod J. 2006;39:626–36.CrossRefPubMed

36.

Cirrincione C, Pimpinelli N, Orlando L, Romagnoli P. Lamina propria dendritic cells express activation markers and contact lymphocytes in chronic periodontitis. J Periodontol. 2002;73:45–52.CrossRefPubMed

37.

Ebersole JL, Holt SC, Hansard R, Novak MJ. Microbiologic and immunologic characteristics of periodontal disease in Hispanic Americans with type 2 diabetes. J Periodontol. 2008;79:637–46.CrossRefPubMed

38.

Ebner S, Ratzinger G, Krosbacher B, Schmuth M, Weiss A, Reider D, et al. Production of IL-12 by human monocyte-derived dendritic cells is optimal when the stimulus is given at the onset of maturation, and is further enhanced by IL-4. J Immunol. 2001;166:633–41.PubMed

39.

Kalinski P, Schuitemaker JH, Hilkens CM, Wierenga EA, Kapsenberg ML. Final maturation of dendritic cells is associated with impaired responsiveness to IFN-gamma and to bacterial IL-12 inducers: decreased ability of mature dendritic cells to produce IL-12 during the interaction with Th cells. J Immunol. 1999;162:3231–6.PubMed

40.

Tassi S, Carta S, Vene R, Delfino L, Ciriolo MR, Rubartelli A. Pathogen-induced interleukin-1beta processing and secretion is regulated by a biphasic redox response. J Immunol. 2009;183:1456–62.CrossRefPubMed

41.

Mogensen TH. Pathogen recognition and inflammatory signaling in innate immune defenses. Clin Microbiol Rev. 2009;22:240–73. Table of contents.CrossRefPubMed

42.

Komai-Koma M, Gilchrist DS, Xu D. Direct recognition of LPS by human but not murine CD8+ T cells via TLR4 complex. Eur J Immunol. 2009;39:1564–72.CrossRefPubMed

43.

Ferstl R, Spiller S, Fichte S, Dreher S, Kirschning CJ. Experimental models of acute infection and Toll-like receptor driven septic shock. Methods Mol Biol. 2009;517:313–27.PubMed

44.

Yun PL, Decarlo AA, Hunter N. Gingipains of Porphyromonas gingivalis modulate leukocyte adhesion molecule expression induced in human endothelial cells by ligation of CD99. Infect Immun. 2006;74:1661–72.CrossRefPubMed

45.

Reife RA, Coats SR, Al-Qutub M, Dixon DM, Braham PA, Billharz RJ, et al. Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity: differential activities of tetra- and penta-acylated lipid A structures on E-selectin expression and TLR4 recognition. Cell Microbiol. 2006;8:857–68.CrossRefPubMed

46.

Nichols FC, Rojanasomsith K. Porphyromonas gingivalis lipids and diseased dental tissues. Oral Microbiol Immunol. 2006;21:84–92.CrossRefPubMed

47.

Naito M, Sakai E, Shi Y, Ideguchi H, Shoji M, Ohara N, et al. Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase. Mol Microbiol. 2006;59:152–67.CrossRefPubMed

48.

Mahanonda R, Pothiraksanon P, Sa-Ard-Iam N, Yamazaki K, Schifferle RE, Hirunpetcharat C, et al. The effects of Porphyromonas gingivalis LPS and Actinobacillus actinomycetemcomitans LPS on human dendritic cells in vitro, and in a mouse model in vivo. Asian Pac J Allergy Immunol. 2006;24:223–8.PubMed

49.

Harokopakis E, Albzreh MH, Martin MH, Hajishengallis G. TLR2 transmodulates monocyte adhesion and transmigration via Rac1- and PI3K-mediated inside-out signaling in response to Porphyromonas gingivalis fimbriae. J Immunol. 2006;176:7645–56.PubMed

50.

Eick S, Reissmann A, Rodel J, Schmidt KH, Pfister W. Porphyromonas gingivalis survives within KB cells and modulates inflammatory response. Oral Microbiol Immunol. 2006;21:231–7.CrossRefPubMed

51.

Bainbridge BW, Coats SR, Darveau RP. Porphyromonas gingivalis lipopolysaccharide displays functionally diverse interactions with the innate host defense system. Ann Periodontol. 2002;7:29–37.CrossRefPubMed

52.

Bainbridge BW, Darveau RP. Porphyromonas gingivalis lipopolysaccharide: an unusual pattern recognition receptor ligand for the innate host defense system. Acta Odontol Scand. 2001;59:131–8.CrossRefPubMed

53.

Coats SR, Do CT, Karimi-Naser LM, Braham PH, Darveau RP. Antagonistic lipopolysaccharides block E coli lipopolysaccharide function at human TLR4 via interaction with the human MD-2 lipopolysaccharide binding site. Cell Microbiol. 2007;9:1191–202.CrossRefPubMed

54.

Huang CB, Altimova Y, Ebersole JL. HIV-1 Promoter activation in dendritic cells by oral microorganisms and LPS. Cell Immunol (submitted).

55.

Huang CB, Emerson KA, Gonzalez OA, Ebersole JL. Oral bacteria induce a differential activation of HIV-1 promoter in T cells, macrophages, and dendritic cells. Oral Microbiol Immunol. 2009;24:401–7.

56.

Blander JM. Signalling and phagocytosis in the orchestration of host defence. Cell Microbiol. 2007;9:290–9.CrossRefPubMed

57.

Blander JM. Coupling Toll-like receptor signaling with phagocytosis: potentiation of antigen presentation. Trends Immunol. 2007;28:19–25.CrossRefPubMed

58.

Le Naour F, Hohenkirk L, Grolleau A, Misek DE, Lescure P, Geiger JD, et al. Profiling changes in gene expression during differentiation and maturation of monocyte-derived dendritic cells using both oligonucleotide microarrays and proteomics. J Biol Chem. 2001;276:17920–31.CrossRefPubMed

59.

Visintin A, Mazzoni A, Spitzer JH, Wyllie DH, Dower SK, Segal DM. Regulation of Toll-like receptors in human monocytes and dendritic cells. J Immunol. 2001;166:249–55.PubMed

60.

van Kooyk Y. C-type lectins on dendritic cells: key modulators for the induction of immune responses. Biochem Soc Trans. 2008;36:1478–81.CrossRefPubMed

Titel: Polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells
verfasst von: C. B. Huang
Y. Altimova
S. Strange
J. L. Ebersole
Publikationsdatum: 01.02.2011
Verlag: SP Birkhäuser Verlag Basel
Erschienen in: Inflammation Research / Ausgabe 2/2011
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-010-0242-0

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Polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells

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