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01.08.2011 | Original Research Paper

G1 cell cycle arrest signaling in hepatic injury after intraperitoneal sepsis in rats

verfasst von: Quan-hui Yang, Da-wei Liu, Xiao-ting Wang, Rong-li Yang, Yan Shi, Yun Long, Hong-zhong Liu, Huai-wu He, Xiang Zhou, Bo Tang

Erschienen in: Inflammation Research | Ausgabe 8/2011

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Abstract

Objective and design

Hepatocytes emerge from a quiescent state into a proliferative state to recover from septic injury. We hypothesize that hepatocyte cell cycle regulation after sepsis potentially contributes to the recovery of liver function.

Methods

An animal model of sepsis was induced by cecal ligation and puncture (CLP) in rats. At serial time points after CLP, hepatocyte expression of p21, P53, cyclin D1, cyclin E, CDK2, CDK4 and PCNA was determined by immunoblot analysis, and the DNA content of isolated hepatocytes was analyzed using flow cytometry.

Results

Sepsis-induced liver injury of rats was associated with G1 cell cycle arrest. Recovery of liver function was related to cell cycle progression 48 h after CLP. The upregulation of p53 and p21 correlated with G1 cell arrest 48 h after CLP. The upregulation of cyclin D1/CDK4 and cyclin E/CDK2 also correlated with the G1/S transition 48 h after CLP, resulting in PCNA expression.

Conclusions

The data suggests that G1 cell cycle arrest and p53, p21, CDKs, cyclins and PCNA expression may be involved in the injury/recovery of liver function after intraperitoneal sepsis.

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Titel: G1 cell cycle arrest signaling in hepatic injury after intraperitoneal sepsis in rats
verfasst von: Quan-hui Yang
Da-wei Liu
Xiao-ting Wang
Rong-li Yang
Yan Shi
Yun Long
Hong-zhong Liu
Huai-wu He
Xiang Zhou
Bo Tang
Publikationsdatum: 01.08.2011
Verlag: SP Birkhäuser Verlag Basel
Erschienen in: Inflammation Research / Ausgabe 8/2011
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-011-0334-5

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G1 cell cycle arrest signaling in hepatic injury after intraperitoneal sepsis in rats

Abstract

Objective and design

Methods

Results

Conclusions

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Parodontalbehandlung verbessert Prognose bei Katheterablation

Antihypertensiva schützen auch alte Menschen noch vor Demenz

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Denken Sie bei starker Blutung auch an die Hemmkörper-Hämophilie

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Objective and design

Methods

Results

Conclusions

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