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01.11.2007 | Article

Increased skeletal muscle ceramide level in men at risk of developing type 2 diabetes

verfasst von: M. Straczkowski, I. Kowalska, M. Baranowski, A. Nikolajuk, E. Otziomek, P. Zabielski, A. Adamska, A. Blachnio, J. Gorski, M. Gorska

Erschienen in: Diabetologia | Ausgabe 11/2007

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Abstract

Aims/hypothesis

Intramyocellular lipids, including ceramide, a second messenger in the sphingomyelin signalling pathway, might contribute to the development of insulin resistance. The aim of our study was to assess parameters of the skeletal muscle sphingomyelin signalling pathway in men at risk of developing type 2 diabetes.

Methods

We studied 12 lean (BMI < 25 kg/m²) men without a family history of diabetes (control group), 12 lean male offspring of type 2 diabetic patients, and 21 men with overweight or obesity comprising 12 with NGT (obese-NGT) and nine with IGT (obese-IGT). A euglycaemic-hyperinsulinaemic clamp and a biopsy of vastus lateralis muscle were performed. Ceramide, sphingomyelin, sphinganine and sphingosine levels and sphingomyelinase and ceramidase activities were measured in muscle. Muscle diacylglycerol and triacylglycerol levels were estimated in a subgroup of 27 men (comprising men from all the above groups).

Results

Compared with the control group, the lean offspring of diabetic patients and the men with overweight or obesity showed lower insulin sensitivity (all p < 0.005) and a greater muscle ceramide level (all p < 0.01). The obese-IGT group had lower insulin sensitivity (p = 0.0018) and higher muscle ceramide (p = 0.0022) than the obese-NGT group. There was lower muscle sphingosine level and alkaline ceramidase activity in offspring of diabetic patients (p = 0.038 and p = 0.031, respectively) and higher sphinganine level in the obese-NGT (p = 0.049) and obese-IGT (p = 0.002) groups than in the control group. Muscle sphingomyelin was lower (p = 0.0028) and neutral sphingomyelinase activity was higher (p = 0.00079) in the obese-IGT than in the obese-NGT group. Muscle ceramide was related to insulin sensitivity independently of other muscle lipid fractions.

Conclusions/interpretations

Ceramide accumulates in muscle of men at risk of developing type 2 diabetes.

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Titel: Increased skeletal muscle ceramide level in men at risk of developing type 2 diabetes
verfasst von: M. Straczkowski
I. Kowalska
M. Baranowski
A. Nikolajuk
E. Otziomek
P. Zabielski
A. Adamska
A. Blachnio
J. Gorski
M. Gorska
Publikationsdatum: 01.11.2007
Verlag: Springer Berlin Heidelberg
Erschienen in: Diabetologia / Ausgabe 11/2007
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-007-0781-2

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Increased skeletal muscle ceramide level in men at risk of developing type 2 diabetes

Abstract

Aims/hypothesis

Methods

Results

Conclusions/interpretations

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Abstract

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