Introduction
Methods
Cohort and patient characteristics
Quantification of endothelial progenitor cells in peripheral blood
Isolation, cultivation and stimulation of leucocytes and subpopulations
Analysis of NAMPT and SIRT1 mRNA expression
Quantification of NAMPT and enzyme activity in serum samples, cell lysates and supernatant fractions
Statistical analyses
Results
Association of NAMPT with normal development in healthy lean children
Association of NAMPT with obesity and obesity interventions
Variable | Univariate correlation | Partial correlation | ||
---|---|---|---|---|
r
| p value |
r
a
| pa value | |
Anthropometric variables | ||||
BMI SDS | 0.31 | <0.0001 | ||
BMI (kg/m2) | 0.35 | <0.0001 | ||
Serum leptin (ng/ml)b
| 0.25 | 0.007 | ||
WHR | 0.06 | 0.426 | ||
Skinfold thickness (cm) | 0.31 | <0.0001 | ||
Metabolic variables | ||||
Fasting BG (mmol/l) | 0.26 | 0.001 | 0.07 | 0.401 |
120 min BG (mmol/l) | 0.20 | 0.012 | 0.13 | 0.129 |
Mean BG (mmol/l) | 0.25 | 0.001 | 0.21 | 0.009 |
AUC BG (mmol/l × min) | 0.28 | <0.0001 | 0.23 | 0.004 |
FPIb (pmol/l) | 0.26 | 0.001 | 0.08 | 0.346 |
Peak insulinb (pmol/l) | 0.24 | 0.003 | 0.10 | 0.237 |
AUC
Ins
b
(pmol/l × min) | 0.27 | 0.001 | 0.13 | 0.124 |
HOMA-IRb
| 0.23 | 0.004 | 0.11 | 0.395 |
Matsuda ISIb
| −0.29 | <0.0001 | −0.09 | 0.112 |
Belfiore ISI | −0.31 | <0.0001 | −0.19 | 0.021 |
AUCIns/AUCBG (pmol/mmol) | 0.24 | 0.003 | 0.10 | 0.215 |
Cardiovascular variables | ||||
Systolic BP (mmHg) | 0.20 | 0.012 | 0.09 | 0.241 |
Diastolic BP (mmHg) | 0.09 | 0.226 | 0.03 | 0.676 |
Mean systolic BP in 24 h (mmHg) | 0.18 | 0.043 | 0.06 | 0.491 |
Mean diastolic BP in 24 h (mmHg) | 0.10 | 0.247 | 0.06 | 0.515 |
IMT (cm) | 0.11 | 0.174 | −0.04 | 0.582 |
Endothelial function (RHI) | −0.23 | 0.004 | −0.10 | 0.232 |
EPC count (per 50,0000 counts)b
| −0.29 | <0.0001 | −0.25 | 0.002 |
EPC migrationb
| 0.01 | 0.871 | 0.04 | 0.669 |
WBC count (×109/l)b
| 0.49 | <0.0001 | 0.44 | <0.0001 |
hsCrP (ng/ml)b
| 0.34 | <0.0001 | 0.27 | 0.001 |
Association of NAMPT with glucose metabolism
Association of NAMPT with cardiovascular parameters and leucocytes
Step | Variable |
R
2
| β ± SEM | B ± SEM | p value |
---|---|---|---|---|---|
Dependent variable: log EPC counta
| |||||
1 | log NAMPT | 0.08 | −0.22 ± 0.10 | −0.23 ± 0.10 | 0.023 |
2 | Leptin | 0.03 | −0.16 ± 0.10 | −0.007 ± 0.004 | 0.095 |
3 | Systolic BP SDS | 0.01 | −0.12 ± 0.09 | −0.07 ± 0.06 | 0.197 |
Dependent variable: log WBC countb
| |||||
1 | log NAMPT | 0.240 | 0.35 ± 0.08 | 0.15 ± 0.03 | <0.001 |
2 | log AUCIns
| 0.105 | 0.40 ± 0.10 | 0.19 ± 0.05 | <0.001 |
3 | log EPC count | 0.022 | −0.16 ± 0.08 | −0.06 ± 0.03 | 0.045 |
4 | Leptin | 0.015 | −0.21 ± 0.10 | −0.003 ± 0.002 | 0.046 |
5 | log hsCrP | 0.014 | 0.13 ± 0.09 | 0.02 ± 0.01 | 0.124 |
Dependent variable: log Matsuda ISIc
| |||||
1 | BMI SDS | 0.360 | −0.54 ± 0.06 | −0.23 ± 0.03 | <0.001 |
2 | log WBC | 0.070 | −0.26 ± 0.06 | 0.56 ± 0.14 | <0.001 |
3 | Age | 0.032 | −0.17 ± 0.06 | −0.03 ± 0.01 | 0.006 |
4 | Sex | 0.008 | 0.09 ± 0.06 | 0.11 ± 0.07 | 0.140 |
Step | Variable |
R
2
| β ± SEM | B ± SEM | p value |
---|---|---|---|---|---|
Dependent variable: log EPC counta
| |||||
1 | log NAMPT | 0.08 | −0.22 ± 0.10 | −0.23 ± 0.10 | 0.023 |
2 | Leptin | 0.03 | −0.16 ± 0.10 | −0.007 ± 0.004 | 0.095 |
3 | Systolic BP SDS | 0.01 | −0.12 ± 0.09 | −0.07 ± 0.06 | 0.197 |
Dependent variable: log WBC countb
| |||||
1 | log NAMPT | 0.240 | 0.35 ± 0.08 | 0.15 ± 0.03 | <0.001 |
2 | log AUCIns
| 0.105 | 0.40 ± 0.10 | 0.19 ± 0.05 | <0.001 |
3 | log EPC count | 0.022 | −0.16 ± 0.08 | −0.06 ± 0.03 | 0.045 |
4 | Leptin | 0.015 | −0.21 ± 0.10 | −0.003 ± 0.002 | 0.046 |
5 | log hsCrP | 0.014 | 0.13 ± 0.09 | 0.02 ± 0.01 | 0.124 |
Dependent variable: log Matsuda ISIc
| |||||
1 | BMI SDS | 0.360 | −0.54 ± 0.06 | −0.23 ± 0.03 | <0.001 |
2 | log WBC | 0.070 | −0.26 ± 0.06 | 0.56 ± 0.14 | <0.001 |
3 | Age | 0.032 | −0.17 ± 0.06 | −0.03 ± 0.01 | 0.006 |
4 | Sex | 0.008 | 0.09 ± 0.06 | 0.11 ± 0.07 | 0.140 |