Erschienen in:
01.10.2015 | Article
p66Shc deletion or deficiency protects from obesity but not metabolic dysfunction in mice and humans
verfasst von:
Stefano Ciciliot, Mattia Albiero, Lisa Menegazzo, Nicol Poncina, Valentina Scattolini, Andrea Danesi, Elisa Pagnin, Manuela Marabita, Bert Blaauw, Marco Giorgio, Mirella Trinei, Mirto Foletto, Luca Prevedello, Donato Nitti, Angelo Avogaro, Gian Paolo Fadini
Erschienen in:
Diabetologia
|
Ausgabe 10/2015
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Abstract
Aims/hypothesis
Oxygen radicals generated by p66Shc drive adipogenesis, but contradictory data exist on the role of p66Shc in the development of obesity and the metabolic syndrome. We herein explored the relationships among p66Shc, adipose tissue remodelling and glucose metabolism using mouse models and human adipose tissue samples.
Methods
In wild-type (WT), leptin-deficient (ob/ob), p66Shc
−/− and p66Shc
−/−
ob/ob mice up to 30 weeks of age, we analysed body weight, subcutaneous and visceral adipose tissue histopathology, glucose tolerance and insulin sensitivity, and liver and muscle fat accumulation. A group of mice on a high fat diet (HFD) was also analysed. A parallel study was conducted on adipose tissue collected from patients undergoing elective surgery.
Results
We found that p66Shc
−/− mice were slightly leaner than WT mice, and p66Shc
−/−
ob/ob mice became less obese than ob/ob mice. Despite their lower body weight, p66Shc
−/− mice accumulated ectopic fat in the liver and muscles, and were glucose intolerant and insulin resistant. Features of adverse adipose tissue remodelling induced by obesity, including adipocyte enlargement, apoptosis, inflammation and perfusion were modestly and transiently improved by p66Shc (also known as Shc1) deletion. After 12 weeks of the HFD, p66Shc
−/− mice were leaner than but equally glucose intolerant and insulin resistant compared with WT mice. In 77 patients, we found a direct correlation between BMI and p66Shc protein levels. Patients with low p66Shc levels were less obese, but were not protected from other metabolic syndrome features (diabetes, dyslipidaemia and hypertension).
Conclusions/interpretation
In mice and humans, reduced p66Shc levels protect from obesity, but not from ectopic fat accumulation, glucose intolerance and insulin resistance.