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Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy 4/2015

01.04.2015 | Knee

Increased platelet concentration does not improve functional graft healing in bio-enhanced ACL reconstruction

verfasst von: Braden C. Fleming, Benedikt L. Proffen, Patrick Vavken, Matthew R. Shalvoy, Jason T. Machan, Martha M. Murray

Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy | Ausgabe 4/2015

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Abstract

Purpose

The use of an extracellular matrix scaffold (ECM) combined with platelets to enhance healing of an anterior cruciate ligament (ACL) graft (“bio-enhanced ACL reconstruction”) has shown promise in animal models. However, the effects of platelet concentration on graft healing remain unknown. The objectives of this study were to determine whether increasing the platelet concentration in the ECM scaffold would (1) improve the graft biomechanical properties and (2) decrease cartilage damage after surgery.

Methods

Fifty-five adolescent minipigs were randomized to five treatment groups: untreated ACL transection (n = 10), conventional ACL reconstruction (n = 15) and bio-enhanced ACL reconstruction using 1× (n = 10), 3× (n = 10) or 5× (n = 10) platelet-rich plasma. The graft biomechanical properties, anteroposterior (AP) knee laxity, graft histology and macroscopic cartilage integrity were measured at 15 weeks.

Results

The mean linear stiffness of the bio-enhanced ACL reconstruction procedure using the 1× preparation was significantly greater than traditional reconstruction, while the 3× and 5× preparations were not. The failure loads of all the ACL-reconstructed groups were equivalent but significantly greater than untreated ACL transection. There were no significant differences in the Ligament Maturity Index or AP laxity between reconstructed knees. Macroscopic cartilage damage was relatively minor, though significantly less when the ECM-platelet composite was used.

Conclusions

Only the 1× platelet concentration improved healing over traditional ACL reconstruction. Increasing the platelet concentration from 1× to 5× in the ECM scaffold did not further improve the graft mechanical properties. The use of an ECM-platelet composite decreased the amount of cartilage damage seen after ACL surgery.
Literatur
1.
Zurück zum Zitat Boguszewski DV, Shearn JT, Wagner CT, Butler DL (2011) Investigating the effects of anterior tibial translation on anterior knee force in the porcine model: is the porcine knee ACL dependent? J Orthop Res 29(5):641–646CrossRefPubMed Boguszewski DV, Shearn JT, Wagner CT, Butler DL (2011) Investigating the effects of anterior tibial translation on anterior knee force in the porcine model: is the porcine knee ACL dependent? J Orthop Res 29(5):641–646CrossRefPubMed
2.
Zurück zum Zitat Boswell SG, Cole BJ, Sundman EA, Karas V, Fortier LA (2012) Platelet-rich plasma: a milieu of bioactive factors. Arthroscopy 28(3):429–439CrossRefPubMed Boswell SG, Cole BJ, Sundman EA, Karas V, Fortier LA (2012) Platelet-rich plasma: a milieu of bioactive factors. Arthroscopy 28(3):429–439CrossRefPubMed
3.
Zurück zum Zitat Butler DL, Lewis JL, Frank CB, Banes AJ, Caplan AI, DeDeyne PG, Dowling M, Fleming BC, Glowacki J, Guldbery RE, Johnstone B, Kaplan DL, Levenston ME, J.C. L, Lu EY, Lumelsky N, Mao JJ, Mauck RL, McDevitt CA, Mejia LC, Murray MM, Ratcliffe A, Spindler KP, Tashman S, Wagner CT, Weisberg EM, Williams C, Zhang R (2008) Evaluation criteria for musculoskeletal and craniofacial tissue engineering constructs: A conference report. Tissue Eng Part A 14 (12):2089–2104 Butler DL, Lewis JL, Frank CB, Banes AJ, Caplan AI, DeDeyne PG, Dowling M, Fleming BC, Glowacki J, Guldbery RE, Johnstone B, Kaplan DL, Levenston ME, J.C. L, Lu EY, Lumelsky N, Mao JJ, Mauck RL, McDevitt CA, Mejia LC, Murray MM, Ratcliffe A, Spindler KP, Tashman S, Wagner CT, Weisberg EM, Williams C, Zhang R (2008) Evaluation criteria for musculoskeletal and craniofacial tissue engineering constructs: A conference report. Tissue Eng Part A 14 (12):2089–2104
4.
Zurück zum Zitat Cervellin M, de Girolamo L, Bait C, Denti M, Volpi P (2012) Autologous platelet-rich plasma gel to reduce donor-site morbidity after patellar tendon graft harvesting for anterior cruciate ligament reconstruction: a randomized, controlled clinical study. Knee Surg Sports Traumatol Arthrosc 20(1):114–120CrossRefPubMed Cervellin M, de Girolamo L, Bait C, Denti M, Volpi P (2012) Autologous platelet-rich plasma gel to reduce donor-site morbidity after patellar tendon graft harvesting for anterior cruciate ligament reconstruction: a randomized, controlled clinical study. Knee Surg Sports Traumatol Arthrosc 20(1):114–120CrossRefPubMed
5.
Zurück zum Zitat de Girolamo L, Galliera E, Volpi P, Denti M, Dogliotti G, Quaglia A, Cabitza P, Corsi Romanelli MM, Randelli P (2013) Why menisci show higher healing rate when repaired during ACL reconstruction? Growth factors release can be the explanation. Knee Surg Sports Traumatol Arthrosc. doi:10.1007/s00167-013-2712-8 de Girolamo L, Galliera E, Volpi P, Denti M, Dogliotti G, Quaglia A, Cabitza P, Corsi Romanelli MM, Randelli P (2013) Why menisci show higher healing rate when repaired during ACL reconstruction? Growth factors release can be the explanation. Knee Surg Sports Traumatol Arthrosc. doi:10.​1007/​s00167-013-2712-8
6.
Zurück zum Zitat Drez DJ, DeLee J, Holden JP, Arnoczky SP, Noyes FR, Roberts TS (1991) Anterior cruciate ligament reconstruction using bone-patellar tendon-bone allografts. A biological and biomechanical evaluation in goats. Am J Sports Med 19(3):256–263CrossRefPubMed Drez DJ, DeLee J, Holden JP, Arnoczky SP, Noyes FR, Roberts TS (1991) Anterior cruciate ligament reconstruction using bone-patellar tendon-bone allografts. A biological and biomechanical evaluation in goats. Am J Sports Med 19(3):256–263CrossRefPubMed
7.
Zurück zum Zitat Dustmann M, Schmidt T, Gangey I, Unterhauser FN, Weiler A, Scheffler SU (2008) The extracellular remodeling of free-soft-tissue autografts and allografts for reconstruction of the anterior cruciate ligament: a comparison study in a sheep model. Knee Surg Sports Traumatol Arthrosc 16(4):360–369CrossRefPubMed Dustmann M, Schmidt T, Gangey I, Unterhauser FN, Weiler A, Scheffler SU (2008) The extracellular remodeling of free-soft-tissue autografts and allografts for reconstruction of the anterior cruciate ligament: a comparison study in a sheep model. Knee Surg Sports Traumatol Arthrosc 16(4):360–369CrossRefPubMed
8.
Zurück zum Zitat Figueroa D, Melean P, Calvo R, Vaisman A, Zilleruelo N, Figueroa F, Villalon I (2010) Magnetic resonance imaging evaluation of the integration and maturation of semitendinosus-gracilis graft in anterior cruciate ligament reconstruction using autologous platelet concentrate. Arthroscopy 26(10):1318–1325CrossRefPubMed Figueroa D, Melean P, Calvo R, Vaisman A, Zilleruelo N, Figueroa F, Villalon I (2010) Magnetic resonance imaging evaluation of the integration and maturation of semitendinosus-gracilis graft in anterior cruciate ligament reconstruction using autologous platelet concentrate. Arthroscopy 26(10):1318–1325CrossRefPubMed
9.
Zurück zum Zitat Fleming BC, Carey JL, Spindler KP, Murray MM (2008) Can suture repair of ACL transection restore normal anterioposterior laxity of the knee? An ex vivo study. J Orthop Res 26(11):1500–1505CrossRefPubMedCentralPubMed Fleming BC, Carey JL, Spindler KP, Murray MM (2008) Can suture repair of ACL transection restore normal anterioposterior laxity of the knee? An ex vivo study. J Orthop Res 26(11):1500–1505CrossRefPubMedCentralPubMed
10.
11.
Zurück zum Zitat Fleming BC, Spindler KP, Palmer MP, Magarian EM, Murray MM (2009) Collagen-platelet composites improve the biomechanical properties of healing anterior cruciate ligament grafts in a porcine model. Am J Sports Med 37(8):1554–1563CrossRefPubMedCentralPubMed Fleming BC, Spindler KP, Palmer MP, Magarian EM, Murray MM (2009) Collagen-platelet composites improve the biomechanical properties of healing anterior cruciate ligament grafts in a porcine model. Am J Sports Med 37(8):1554–1563CrossRefPubMedCentralPubMed
12.
Zurück zum Zitat Foster TE, Puskas BL, Mandelbaum BR, Gerhardt MB, Rodeo SA (2009) Platelet-rich plasma: from basic science to clinical applications. Am J Sports Med 37(11):2259–2272CrossRefPubMed Foster TE, Puskas BL, Mandelbaum BR, Gerhardt MB, Rodeo SA (2009) Platelet-rich plasma: from basic science to clinical applications. Am J Sports Med 37(11):2259–2272CrossRefPubMed
13.
Zurück zum Zitat Frank CB, Beveridge JE, Huebner KD, Heard BJ, Tapper JE, O’Brien EJ, Shrive NG (2012) Complete ACL/MCL deficiency induces variable degrees of instability in sheep with specific kinematic abnormalities correlating with degrees of early osteoarthritis. J Orthop Res 30(3):384–392CrossRefPubMed Frank CB, Beveridge JE, Huebner KD, Heard BJ, Tapper JE, O’Brien EJ, Shrive NG (2012) Complete ACL/MCL deficiency induces variable degrees of instability in sheep with specific kinematic abnormalities correlating with degrees of early osteoarthritis. J Orthop Res 30(3):384–392CrossRefPubMed
14.
Zurück zum Zitat Jackson DW, Grood ES, Goldstein JD, Rosen MA, Kurzweil PR, Cummings JF, Simon TM (1993) A comparison of patellar tendon autograft and allograft used for anterior cruciate ligament reconstruction in the goat model. Am J Sports Med 21(2):176–185CrossRefPubMed Jackson DW, Grood ES, Goldstein JD, Rosen MA, Kurzweil PR, Cummings JF, Simon TM (1993) A comparison of patellar tendon autograft and allograft used for anterior cruciate ligament reconstruction in the goat model. Am J Sports Med 21(2):176–185CrossRefPubMed
15.
Zurück zum Zitat Jackson DW, McDevitt CA, Simon TM, Arnoczky SP, Atwell EA, Silvino NJ (1992) Meniscal transplantation using fresh and cryopreserved allografts. An experimental study in goats. Am J Sports Med 20(6):644–656CrossRefPubMed Jackson DW, McDevitt CA, Simon TM, Arnoczky SP, Atwell EA, Silvino NJ (1992) Meniscal transplantation using fresh and cryopreserved allografts. An experimental study in goats. Am J Sports Med 20(6):644–656CrossRefPubMed
16.
Zurück zum Zitat Joshi S, Mastrangelo A, Magarian E, Fleming BC, Murray MM (2009) Collagen-platelet composite enhances biomechanical and histologic healing of the porcine ACL. Am J Sports Med 37(12):2401–2410CrossRefPubMedCentralPubMed Joshi S, Mastrangelo A, Magarian E, Fleming BC, Murray MM (2009) Collagen-platelet composite enhances biomechanical and histologic healing of the porcine ACL. Am J Sports Med 37(12):2401–2410CrossRefPubMedCentralPubMed
17.
Zurück zum Zitat Katsuragi R, Yasuda K, Tsujino J, Keira M, Kaneda K (2000) The effect of nonphysiologically high initial tension on the mechanical properties of in situ frozen anterior cruciate ligament in a canine model. Am J Sports Med 28(1):47–56PubMed Katsuragi R, Yasuda K, Tsujino J, Keira M, Kaneda K (2000) The effect of nonphysiologically high initial tension on the mechanical properties of in situ frozen anterior cruciate ligament in a canine model. Am J Sports Med 28(1):47–56PubMed
18.
Zurück zum Zitat Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M (2010) Platelet-rich plasma: intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 18(4):472–479CrossRefPubMed Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M (2010) Platelet-rich plasma: intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 18(4):472–479CrossRefPubMed
19.
Zurück zum Zitat Luck JV Jr, Silva M, Rodriguez-Merchan EC, Ghalambor N, Zahiri CA, Finn RS (2004) Hemophilic arthropathy. J Am Acad Orthop Surg 12(4):234–245PubMed Luck JV Jr, Silva M, Rodriguez-Merchan EC, Ghalambor N, Zahiri CA, Finn RS (2004) Hemophilic arthropathy. J Am Acad Orthop Surg 12(4):234–245PubMed
20.
Zurück zum Zitat Magarian EM, Vavken P, Connolly SA, Mastrangelo AN, Murray MM (2012) Safety of intra-articular use of atelocollagen for enhanced tissue repair. Open Orthop J 6:231–238CrossRefPubMedCentralPubMed Magarian EM, Vavken P, Connolly SA, Mastrangelo AN, Murray MM (2012) Safety of intra-articular use of atelocollagen for enhanced tissue repair. Open Orthop J 6:231–238CrossRefPubMedCentralPubMed
21.
Zurück zum Zitat Magarian EM, Vavken P, Murray MM (2011) Human anterior cruciate ligament fibroblasts from immature patients have a stronger in vitro response to platelet concentrates than those from mature individuals. Knee 18(4):247–251CrossRefPubMedCentralPubMed Magarian EM, Vavken P, Murray MM (2011) Human anterior cruciate ligament fibroblasts from immature patients have a stronger in vitro response to platelet concentrates than those from mature individuals. Knee 18(4):247–251CrossRefPubMedCentralPubMed
22.
Zurück zum Zitat Mastrangelo AN, Haus BM, Vavken P, Palmer MP, Machan JT, Murray MM (2010) Immature animals have higher cellular density in the healing anterior cruciate ligament than adolescent or adult animals. J Orthop Res 28(8):1100–1106PubMedCentralPubMed Mastrangelo AN, Haus BM, Vavken P, Palmer MP, Machan JT, Murray MM (2010) Immature animals have higher cellular density in the healing anterior cruciate ligament than adolescent or adult animals. J Orthop Res 28(8):1100–1106PubMedCentralPubMed
23.
Zurück zum Zitat Mastrangelo AN, Magarian EM, Palmer MP, Vavken P, Murray MM (2010) The effect of skeletal maturity on the regenerative function of intrinsic ACL cells. J Orthop Res 28(5):644–651PubMedCentralPubMed Mastrangelo AN, Magarian EM, Palmer MP, Vavken P, Murray MM (2010) The effect of skeletal maturity on the regenerative function of intrinsic ACL cells. J Orthop Res 28(5):644–651PubMedCentralPubMed
24.
Zurück zum Zitat Mastrangelo AN, Vavken P, Fleming BC, Harrison SL, Murray MM (2011) Reduced platelet concentration does not harm PRP effectiveness for ACL repair in a porcine in vivo model. J Orthop Res 29(7):1002–1007CrossRefPubMedCentralPubMed Mastrangelo AN, Vavken P, Fleming BC, Harrison SL, Murray MM (2011) Reduced platelet concentration does not harm PRP effectiveness for ACL repair in a porcine in vivo model. J Orthop Res 29(7):1002–1007CrossRefPubMedCentralPubMed
25.
Zurück zum Zitat Mazzocca AD, McCarthy MB, Chowaniec DM, Cote MP, Romeo AA, Bradley JP, Arciero RA, Beitzel K (2012) Platelet-rich plasma differs according to preparation method and human variability. J Bone Joint Surg Am 94(4):308–316CrossRefPubMed Mazzocca AD, McCarthy MB, Chowaniec DM, Cote MP, Romeo AA, Bradley JP, Arciero RA, Beitzel K (2012) Platelet-rich plasma differs according to preparation method and human variability. J Bone Joint Surg Am 94(4):308–316CrossRefPubMed
26.
Zurück zum Zitat Molloy T, Wang Y, Murrell G (2003) The roles of growth factors in tendon and ligament healing. Sports Med 33(5):381–394CrossRefPubMed Molloy T, Wang Y, Murrell G (2003) The roles of growth factors in tendon and ligament healing. Sports Med 33(5):381–394CrossRefPubMed
27.
Zurück zum Zitat Mueller XM, Tevaearai HT, Jegger D, Tucker O, von Segesser LK (2001) Are standard human coagulation tests suitable in pigs and calves during extracorporeal circulation? Artif Organs 25(7):579–584CrossRefPubMed Mueller XM, Tevaearai HT, Jegger D, Tucker O, von Segesser LK (2001) Are standard human coagulation tests suitable in pigs and calves during extracorporeal circulation? Artif Organs 25(7):579–584CrossRefPubMed
28.
Zurück zum Zitat Murray MM, Fleming BC (2013) Biology of anterior cruciate ligament injury and repair: kappa delta Ann doner Vaughn award paper 2013. J Orthop Res 31(10):1501–1506CrossRefPubMedCentralPubMed Murray MM, Fleming BC (2013) Biology of anterior cruciate ligament injury and repair: kappa delta Ann doner Vaughn award paper 2013. J Orthop Res 31(10):1501–1506CrossRefPubMedCentralPubMed
29.
Zurück zum Zitat Murray MM, Fleming BC (2013) Use of a bioactive scaffold to stimulate anterior cruciate ligament healing also minimizes posttraumatic osteoarthritis after surgery. Am J Sports Med 41(8):1762–1770CrossRefPubMedCentralPubMed Murray MM, Fleming BC (2013) Use of a bioactive scaffold to stimulate anterior cruciate ligament healing also minimizes posttraumatic osteoarthritis after surgery. Am J Sports Med 41(8):1762–1770CrossRefPubMedCentralPubMed
30.
Zurück zum Zitat Murray MM, Magarian EM, Harrison SL, Mastrangelo AN, Zurakowski D, Fleming BC (2010) The effect of skeletal maturity on functional healing of the anterior cruciate ligament. J Bone Joint Surg Am 92(11):2039–2049CrossRefPubMedCentralPubMed Murray MM, Magarian EM, Harrison SL, Mastrangelo AN, Zurakowski D, Fleming BC (2010) The effect of skeletal maturity on functional healing of the anterior cruciate ligament. J Bone Joint Surg Am 92(11):2039–2049CrossRefPubMedCentralPubMed
31.
Zurück zum Zitat Murray MM, Palmer M, Abreu E, Spindler KP, Zurakowski D, Fleming BC (2009) Platelet-rich plasma alone is not sufficient to enhance suture repair of the ACL in skeletally immature animals: an in vivo study. J Orthop Res 27(5):639–645CrossRefPubMedCentralPubMed Murray MM, Palmer M, Abreu E, Spindler KP, Zurakowski D, Fleming BC (2009) Platelet-rich plasma alone is not sufficient to enhance suture repair of the ACL in skeletally immature animals: an in vivo study. J Orthop Res 27(5):639–645CrossRefPubMedCentralPubMed
32.
Zurück zum Zitat Myers SL, Brandt KD, O’Connor BL, Visco DM, Albrecht ME (1990) Synovitis and osteoarthritic changes in canine articular cartilage after anterior cruciate ligament transection. Effect of surgical hemostasis. Arthritis Rheum 33(9):1406–1415CrossRefPubMed Myers SL, Brandt KD, O’Connor BL, Visco DM, Albrecht ME (1990) Synovitis and osteoarthritic changes in canine articular cartilage after anterior cruciate ligament transection. Effect of surgical hemostasis. Arthritis Rheum 33(9):1406–1415CrossRefPubMed
33.
Zurück zum Zitat Nin JR, Gasque GM, Azcarate AV, Beola JD, Gonzalez MH (2009) Has platelet-rich plasma any role in anterior cruciate ligament allograft healing? Arthroscopy 25(11):1206–1213CrossRefPubMed Nin JR, Gasque GM, Azcarate AV, Beola JD, Gonzalez MH (2009) Has platelet-rich plasma any role in anterior cruciate ligament allograft healing? Arthroscopy 25(11):1206–1213CrossRefPubMed
34.
Zurück zum Zitat Oiestad BE, Holm I, Aune AK, Gunderson R, Myklebust G, Engebretsen L, Fosdahl MA, Risberg MA (2010) Knee function and prevalence of knee osteoarthritis after anterior cruciate ligament reconstruction: a prospective study with 10–15 years of follow-up. Am J Sports Med 38(11):2201–2210CrossRefPubMed Oiestad BE, Holm I, Aune AK, Gunderson R, Myklebust G, Engebretsen L, Fosdahl MA, Risberg MA (2010) Knee function and prevalence of knee osteoarthritis after anterior cruciate ligament reconstruction: a prospective study with 10–15 years of follow-up. Am J Sports Med 38(11):2201–2210CrossRefPubMed
35.
Zurück zum Zitat Orrego M, Larrain C, Rosales J, Valenzuela L, Matas J, Durruty J, Sudy H, Mardones R (2008) Effects of platelet concentrate and a bone plug on the healing of hamstring tendons in a bone tunnel. Arthroscopy 24(12):1373–1380CrossRefPubMed Orrego M, Larrain C, Rosales J, Valenzuela L, Matas J, Durruty J, Sudy H, Mardones R (2008) Effects of platelet concentrate and a bone plug on the healing of hamstring tendons in a bone tunnel. Arthroscopy 24(12):1373–1380CrossRefPubMed
36.
Zurück zum Zitat Proffen BL, Fleming BC, Murray MM (2013) Histologic predictors of maximum failure loads differ between the healing ACL and ACL grafts after 6 and 12 months in vivo. Orthop J Sports Med 1(6):1–11 Proffen BL, Fleming BC, Murray MM (2013) Histologic predictors of maximum failure loads differ between the healing ACL and ACL grafts after 6 and 12 months in vivo. Orthop J Sports Med 1(6):1–11
37.
Zurück zum Zitat Proffen BL, McElfresh M, Fleming BC, Murray MM (2012) A comparative anatomical study of the human knee and six animal species. Knee 19(4):469–476CrossRef Proffen BL, McElfresh M, Fleming BC, Murray MM (2012) A comparative anatomical study of the human knee and six animal species. Knee 19(4):469–476CrossRef
38.
Zurück zum Zitat Radice F, Yanez R, Gutierrez V, Rosales J, Pinedo M, Coda S (2010) Comparison of magnetic resonance imaging findings in anterior cruciate ligament grafts with and without autologous platelet-derived growth factors. Arthroscopy 26(1):50–57CrossRefPubMed Radice F, Yanez R, Gutierrez V, Rosales J, Pinedo M, Coda S (2010) Comparison of magnetic resonance imaging findings in anterior cruciate ligament grafts with and without autologous platelet-derived growth factors. Arthroscopy 26(1):50–57CrossRefPubMed
39.
Zurück zum Zitat Roosendaal G, Vianen ME, Marx JJ, van den Berg HM, Lafeber FP, Bijlsma JW (1999) Blood-induced joint damage: a human in vitro study. Arthritis Rheum 42(5):1025–1032CrossRefPubMed Roosendaal G, Vianen ME, Marx JJ, van den Berg HM, Lafeber FP, Bijlsma JW (1999) Blood-induced joint damage: a human in vitro study. Arthritis Rheum 42(5):1025–1032CrossRefPubMed
40.
Zurück zum Zitat Sanchez M, Anitua E, Azofra J, Prado R, Muruzabal F, Andia I (2010) Ligamentization of tendon grafts treated with an endogenous preparation rich in growth factors: gross morphology and histology. Arthroscopy 26(4):470–480CrossRefPubMed Sanchez M, Anitua E, Azofra J, Prado R, Muruzabal F, Andia I (2010) Ligamentization of tendon grafts treated with an endogenous preparation rich in growth factors: gross morphology and histology. Arthroscopy 26(4):470–480CrossRefPubMed
41.
Zurück zum Zitat Scheffler SU, Schmidt T, Gangey I, Dustmann M, Unterhauser F, Weiler A (2008) Fresh-frozen free-tendon allografts versus autografts in anterior cruciate ligament reconstruction: delayed remodeling and inferior mechanical function during long-term healing in sheep. Arthroscopy 24(4):448–458CrossRefPubMed Scheffler SU, Schmidt T, Gangey I, Dustmann M, Unterhauser F, Weiler A (2008) Fresh-frozen free-tendon allografts versus autografts in anterior cruciate ligament reconstruction: delayed remodeling and inferior mechanical function during long-term healing in sheep. Arthroscopy 24(4):448–458CrossRefPubMed
42.
Zurück zum Zitat Schinhan M, Gruber M, Vavken P, Dorotka R, Samouh L, Chiari C, Gruebl-Barabas R, Nehrer S (2011) Critical-size defect induces unicompartmental osteoarthritis in a stable ovine knee. J Orthop Res 30(2):214–220CrossRefPubMed Schinhan M, Gruber M, Vavken P, Dorotka R, Samouh L, Chiari C, Gruebl-Barabas R, Nehrer S (2011) Critical-size defect induces unicompartmental osteoarthritis in a stable ovine knee. J Orthop Res 30(2):214–220CrossRefPubMed
43.
Zurück zum Zitat Sheth U, Simunovic N, Klein G, Fu F, Einhorn TA, Schemitsch E, Ayeni OR, Bhandari M (2012) Efficacy of autologous platelet-rich plasma use for orthopaedic indications: a meta-analysis. The J Bone Joint Surg Am 94(4):298–307CrossRef Sheth U, Simunovic N, Klein G, Fu F, Einhorn TA, Schemitsch E, Ayeni OR, Bhandari M (2012) Efficacy of autologous platelet-rich plasma use for orthopaedic indications: a meta-analysis. The J Bone Joint Surg Am 94(4):298–307CrossRef
44.
Zurück zum Zitat Silva A, Sampaio R (2009) Anatomic ACL reconstruction: does the platelet-rich plasma accelerate tendon healing? Knee Surg Sports Traumatol Arthrosc 17(6):676–682CrossRefPubMed Silva A, Sampaio R (2009) Anatomic ACL reconstruction: does the platelet-rich plasma accelerate tendon healing? Knee Surg Sports Traumatol Arthrosc 17(6):676–682CrossRefPubMed
45.
Zurück zum Zitat van Buul GM, Koevoet WL, Kops N, Bos PK, Verhaar JA, Weinans H, Bernsen MR, van Osch GJ (2011) Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes. Am J Sports Med 39(11):2362–2370CrossRefPubMed van Buul GM, Koevoet WL, Kops N, Bos PK, Verhaar JA, Weinans H, Bernsen MR, van Osch GJ (2011) Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes. Am J Sports Med 39(11):2362–2370CrossRefPubMed
46.
Zurück zum Zitat Vavken P, Fleming BC, Mastrangelo AN, Machan JT, Murray MM (2012) Biomechanical outcomes after bioenhanced anterior cruciate ligament repair and anterior cruciate ligament reconstruction are equal in a porcine model. Arthroscopy 28(5):672–680CrossRefPubMedCentralPubMed Vavken P, Fleming BC, Mastrangelo AN, Machan JT, Murray MM (2012) Biomechanical outcomes after bioenhanced anterior cruciate ligament repair and anterior cruciate ligament reconstruction are equal in a porcine model. Arthroscopy 28(5):672–680CrossRefPubMedCentralPubMed
47.
Zurück zum Zitat Vavken P, Joshi S, Murray MM (2009) TRITON-X is most effective among three decellularization agents for ACL tissue engineering. J Orthop Res 27(12):1612–1618CrossRefPubMedCentralPubMed Vavken P, Joshi S, Murray MM (2009) TRITON-X is most effective among three decellularization agents for ACL tissue engineering. J Orthop Res 27(12):1612–1618CrossRefPubMedCentralPubMed
48.
Zurück zum Zitat Vavken P, Saad FA, Murray MM (2010) Age dependence of expression of growth factor receptors in porcine ACL fibroblasts. J Orthop Res 28(8):1107–1112PubMedCentralPubMed Vavken P, Saad FA, Murray MM (2010) Age dependence of expression of growth factor receptors in porcine ACL fibroblasts. J Orthop Res 28(8):1107–1112PubMedCentralPubMed
49.
Zurück zum Zitat Vavken P, Sadoghi P, Murray MM (2011) The effect of platelet concentrates on graft maturation and graft-bone interface healing in anterior cruciate ligament reconstruction in human patients: a systematic review of controlled trials. Arthroscopy 27(11):1573–1583CrossRefPubMedCentralPubMed Vavken P, Sadoghi P, Murray MM (2011) The effect of platelet concentrates on graft maturation and graft-bone interface healing in anterior cruciate ligament reconstruction in human patients: a systematic review of controlled trials. Arthroscopy 27(11):1573–1583CrossRefPubMedCentralPubMed
50.
Zurück zum Zitat Ventura A, Terzaghi C, Borgo E, Verdoia C, Gallazzi M, Failoni S (2005) Use of growth factors in ACL surgery: preliminary study. J Orthop Traumatol 6:76–79CrossRef Ventura A, Terzaghi C, Borgo E, Verdoia C, Gallazzi M, Failoni S (2005) Use of growth factors in ACL surgery: preliminary study. J Orthop Traumatol 6:76–79CrossRef
51.
Zurück zum Zitat Vogrin M, Rupreht M, Dinevski D, Haspl M, Kuhta M, Jevsek M, Knezevic M, Rozman P (2010) Effects of a platelet gel on early graft revascularization after anterior cruciate ligament reconstruction: a prospective, randomized, double-blind, clinical trial. Eur Surg Res 45(2):77–85CrossRefPubMed Vogrin M, Rupreht M, Dinevski D, Haspl M, Kuhta M, Jevsek M, Knezevic M, Rozman P (2010) Effects of a platelet gel on early graft revascularization after anterior cruciate ligament reconstruction: a prospective, randomized, double-blind, clinical trial. Eur Surg Res 45(2):77–85CrossRefPubMed
52.
Zurück zum Zitat Von Porat A, Roos EM, Roos H (2004) High prevalence of osteoarthritis 14 years after an anterior cruciate ligament tear in male soccer players: a study of radiographic and patient relevant outcomes. Ann Rheum Dis 63(3):269–273CrossRef Von Porat A, Roos EM, Roos H (2004) High prevalence of osteoarthritis 14 years after an anterior cruciate ligament tear in male soccer players: a study of radiographic and patient relevant outcomes. Ann Rheum Dis 63(3):269–273CrossRef
53.
Zurück zum Zitat Wei X, Mao Z, Hou Y, Lin L, Xue T, Chen L, Wang H, Yu C (2011) Local administration of TGFbeta-1/VEGF165 gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits. Biochem Biophys Res Commun 406(2):204–210CrossRefPubMed Wei X, Mao Z, Hou Y, Lin L, Xue T, Chen L, Wang H, Yu C (2011) Local administration of TGFbeta-1/VEGF165 gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits. Biochem Biophys Res Commun 406(2):204–210CrossRefPubMed
54.
Zurück zum Zitat Weiler A, Forster C, Hunt P, Falk R, Jung T, Unterhauser FN, Bergmann V, Schmidmaier G, Haas NP (2004) The influence of locally applied platelet-derived growth factor-BB on free tendon graft remodeling after anterior cruciate ligament reconstruction. Am J Sports Med 32(4):881–891CrossRefPubMed Weiler A, Forster C, Hunt P, Falk R, Jung T, Unterhauser FN, Bergmann V, Schmidmaier G, Haas NP (2004) The influence of locally applied platelet-derived growth factor-BB on free tendon graft remodeling after anterior cruciate ligament reconstruction. Am J Sports Med 32(4):881–891CrossRefPubMed
55.
Zurück zum Zitat Xerogeanes JW, Fox RJ, Takeda Y, Hyoung-Soo K, Ishibashi Y, Carlin GJ, Woo SL-Y (1998) A functional comparison of animal anterior cruciate ligament models to the human anterior cruciate ligament. Ann Biomed Engin 26(3):345–352CrossRef Xerogeanes JW, Fox RJ, Takeda Y, Hyoung-Soo K, Ishibashi Y, Carlin GJ, Woo SL-Y (1998) A functional comparison of animal anterior cruciate ligament models to the human anterior cruciate ligament. Ann Biomed Engin 26(3):345–352CrossRef
56.
Zurück zum Zitat Yasuda K, Tomita F, Yamazaki S, Minami A, Tohyama H (2004) The effect of growth factors on biomechanical properties of the bone-patellar tendon-bone graft after anterior cruciate ligament reconstruction: a canine model study. Am J Sports Med 32(4):870–880CrossRefPubMed Yasuda K, Tomita F, Yamazaki S, Minami A, Tohyama H (2004) The effect of growth factors on biomechanical properties of the bone-patellar tendon-bone graft after anterior cruciate ligament reconstruction: a canine model study. Am J Sports Med 32(4):870–880CrossRefPubMed
57.
Zurück zum Zitat Yoshida R, Cheng M, Murray MM (2014) Increasing platelet concentration in platelet-rich plasma inhibits anterior cruciate ligament cell function in three-dimensional culture. J Orthop Res 32(2):291–295CrossRefPubMedCentralPubMed Yoshida R, Cheng M, Murray MM (2014) Increasing platelet concentration in platelet-rich plasma inhibits anterior cruciate ligament cell function in three-dimensional culture. J Orthop Res 32(2):291–295CrossRefPubMedCentralPubMed
58.
Zurück zum Zitat Yoshida R, Vavken P, Murray MM (2012) Decellularization of bovine anterior cruciate ligament tissues minimizes immunogenic reactions to alpha-gal epitopes by human peripheral blood mononuclear cells. Knee 19(5):672–675CrossRefPubMedCentralPubMed Yoshida R, Vavken P, Murray MM (2012) Decellularization of bovine anterior cruciate ligament tissues minimizes immunogenic reactions to alpha-gal epitopes by human peripheral blood mononuclear cells. Knee 19(5):672–675CrossRefPubMedCentralPubMed
59.
Zurück zum Zitat Yoshikawa T, Tohyama H, Enomoto H, Matsumoto H, Toyama Y, Yasuda K (2006) Expression of vascular endothelial growth factor and angiogenesis in patellar tendon grafts in the early phase after anterior cruciate ligament reconstruction. Knee Surg Sports Traumatol Arthrosc 14(9):804–810CrossRefPubMed Yoshikawa T, Tohyama H, Enomoto H, Matsumoto H, Toyama Y, Yasuda K (2006) Expression of vascular endothelial growth factor and angiogenesis in patellar tendon grafts in the early phase after anterior cruciate ligament reconstruction. Knee Surg Sports Traumatol Arthrosc 14(9):804–810CrossRefPubMed
60.
Zurück zum Zitat Yoshikawa T, Tohyama H, Katsura T, Kondo E, Kotani Y, Matsumoto H, Toyama Y, Yasuda K (2006) Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep. Am J Sports Med 34(12):1918–1925CrossRefPubMed Yoshikawa T, Tohyama H, Katsura T, Kondo E, Kotani Y, Matsumoto H, Toyama Y, Yasuda K (2006) Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep. Am J Sports Med 34(12):1918–1925CrossRefPubMed
Metadaten
Titel
Increased platelet concentration does not improve functional graft healing in bio-enhanced ACL reconstruction
verfasst von
Braden C. Fleming
Benedikt L. Proffen
Patrick Vavken
Matthew R. Shalvoy
Jason T. Machan
Martha M. Murray
Publikationsdatum
01.04.2015
Verlag
Springer Berlin Heidelberg
Erschienen in
Knee Surgery, Sports Traumatology, Arthroscopy / Ausgabe 4/2015
Print ISSN: 0942-2056
Elektronische ISSN: 1433-7347
DOI
https://doi.org/10.1007/s00167-014-2932-6

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