Abstract
Canine parvovirus (CPV) can cause acute hemorrhagic diarrhea and fatal myocarditis in young dogs. Currently, most studies have focused on the evolution of the VP2 gene, whereas the full-length genome of CPV has been rarely reported. In this study, the whole genomes of CPV-LZ1 and CPV-LZ2 strains prevalent in Northwest China were determined and analyzed in comparison with those of the reference CPVs. The genome sequences of both LZ strains consisted of 5053 nucleotides. CPV-LZ1 and CPV-LZ2 strains were designated as new CPV-2a and CPV-2b, respectively. Sequence alignment analysis results revealed that these two new strains underwent specific unique variations during the process of local adaption. The left non-translated regions of these strains formed a Y-shaped hairpin structure, whereas the right non-translated regions lacked the reiteration of DNA sequence. A phylogenetic tree constructed from 33 whole coding regions of CPVs showed a strong spatial clustering, and these two strains belonged to the Chinese strain cluster lineage. This study provides a method to obtain the full-length genome of CPV. The isolation and characterization of these viruses adds incrementally to the knowledge of the full-length genome of CPV. The results from this study also provide insight into the molecular epidemiology and genetic diversity of the CPV field isolates from Northwest China and can be useful in preventing and controlling CPV infection in this region.
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Acknowledgments
This research was supported by grants from the International Science & Technology Cooperation Program of China (2014DFA31890), the National Science and Technology Support Program (2013BAD12B00), and the “Twelfth Five-Year” National Science and technology program in rural areas (2011AA10A211). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Communicated by Erko Stackebrandt.
Shi-Chong Han and Hui-Chen Guo contributed equally to this work and should be considered as co-first authors.
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Han, SC., Guo, HC., Sun, SQ. et al. Full-length genomic characterizations of two canine parvoviruses prevalent in Northwest China. Arch Microbiol 197, 621–626 (2015). https://doi.org/10.1007/s00203-015-1093-4
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DOI: https://doi.org/10.1007/s00203-015-1093-4