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Gallic acid functions as a TRPA1 antagonist with relevant antinociceptive and antiedematogenic effects in mice

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Abstract

The transient receptor potential ankyrin 1 (TRPA1) has been identified as a relevant target for the development of novel analgesics. Gallic acid (GA) is a polyphenolic compound commonly found in green tea and various berries and possesses a wide range of biological activities. The goal of this study was to identify GA as a TRPA1 antagonist and observe its antinociceptive effects in different pain models. First, we evaluated the ability of GA to affect cinnamaldehyde-induced calcium influx. Then, we observed the antinociceptive and antiedematogenic effects of GA (3–100 mg/kg) oral administration after the intraplantar (i.pl.) injection of TRPA1 agonists (allyl isothiocyanate, cinnamaldehyde, or hydrogen peroxide—H2O2) in either an inflammatory pain model (carrageenan i.pl. injection) or a neuropathic pain model (chronic constriction injury) in male Swiss mice (25–35 g). GA reduced the calcium influx mediated by TRPA1 activation. Moreover, the oral administration of GA decreased the spontaneous nociception triggered by allyl isothiocyanate, cinnamaldehyde, and H2O2. Carrageenan-induced allodynia and edema were largely reduced by the pretreatment with GA. Moreover, the administration of GA was also capable of decreasing cold and mechanical allodynia in a neuropathic pain model. Finally, GA was absorbed after oral administration and did not produce any detectable side effects. In conclusion, we found that GA is a TRPA1 antagonist with antinociceptive properties in relevant models of clinical pain without detectable side effects, which makes it a good candidate for the treatment of painful conditions.

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Abbreviations

AITC:

Allyl isothiocyanate

CCI:

Chronic constriction injury

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

GA:

Gallic acid

H2O2 :

Hydrogen peroxide

4-HNE:

4-Hydroxynonenal

i.p.:

Intraperitoneal

PWT:

Paw withdrawal threshold

PBS:

Phosphate-buffered saline

p.o.:

Oral route

s.c.:

Subcutaneously

TRPA1:

Transient receptor potential ankyrin 1

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Acknowledgments

Fellowships from Conselho Nacional de Desenvolvimento Científico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) (Brazil) supported this work.

Conflict of interest

The authors declare no competing financial interests.

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Authors and Affiliations

  1. Graduate Program in Biological Sciences: Toxicological Biochemistry, Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria (UFSM), Avenida Roraima, 1000, Camobi, Santa Maria-RS, 97105-900, Brazil

    Gabriela Trevisan, Mateus F. Rossato, Raquel Tonello, Fernanda Rosa & Juliano Ferreira

  2. Graduate Program in Pharmacology, Department of Physiology and Pharmacology, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil

    Carin Hoffmeister, Kelly V. Pinheiro, Francielle V. Pinheiro, Aline A. Boligon, Margareth L. Athayde & Juliano Ferreira

  3. Grupo Multidisciplinar de Saúde, Cruz Alta University (UNICRUZ), Cruz Alta, RS, 98020-290, Brazil

    Jonatas Z. Klafke

  4. Department of Pharmacology, Center of Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88049-900, Brazil

    Juliano Ferreira

  5. Laboratory of Cellular and Molecular Biology, Graduate Program in Health Sciences, University of the Extreme South of Santa Catarina (UNESC), Criciúma, SC, 88806-000, Brazil

    Gabriela Trevisan

  6. Faculdade de Educação e Cultura de Vilhena, Vilhena, RO, 76980-000, Brazil

    Carin Hoffmeister

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  1. Gabriela Trevisan
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  2. Mateus F. Rossato
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  3. Raquel Tonello
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  5. Jonatas Z. Klafke
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  9. Aline A. Boligon
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  10. Margareth L. Athayde
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Correspondence to Gabriela Trevisan.

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Trevisan, G., Rossato, M.F., Tonello, R. et al. Gallic acid functions as a TRPA1 antagonist with relevant antinociceptive and antiedematogenic effects in mice. Naunyn-Schmiedeberg's Arch Pharmacol 387, 679–689 (2014). https://doi.org/10.1007/s00210-014-0978-0

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