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Experimental Brain Research

Erschienen in:

01.03.2007 | Research Article

Desensitization of cold- and menthol-sensitive rat dorsal root ganglion neurones by inflammatory mediators

verfasst von: Ramona Madalina Linte, Cristian Ciobanu, Gordon Reid, Alexandru Babes

Erschienen in: Experimental Brain Research | Ausgabe 1/2007

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Abstract

The interaction between cold sensitivity and inflammation in mammals is not entirely understood. We have used adult rat dorsal root ganglion neurones in primary culture together with calcium microfluorimetry to assess the effects of selected inflammatory mediators on cold responses of cold- and menthol-sensitive (most likely TRPM8-expressing) neurones. We observed a high degree of functional co-expression of TRPM8, the receptors for the inflammatory agents bradykinin, prostaglandin E₂ and histamine, and TRPA1 in cultured sensory neurones. Treatment with either bradykinin or prostaglandin E₂ led to a reduction in the amplitude of the response to cooling and shifted the threshold temperature to colder values, and we provide evidence for a role of protein kinases C and A, respectively, in mediating these effects. In both cases the effects were mainly restricted to the subgroups of cold- and menthol-sensitive cells which had responded to the application of the inflammatory agents at basal temperature. This desensitization of cold-sensitive neurones may enhance inflammatory pain by removing the analgesic effects of gentle cooling.

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Titel: Desensitization of cold- and menthol-sensitive rat dorsal root ganglion neurones by inflammatory mediators
verfasst von: Ramona Madalina Linte
Cristian Ciobanu
Gordon Reid
Alexandru Babes
Publikationsdatum: 01.03.2007
Verlag: Springer-Verlag
Erschienen in: Experimental Brain Research / Ausgabe 1/2007
Print ISSN: 0014-4819
Elektronische ISSN: 1432-1106
DOI: https://doi.org/10.1007/s00221-006-0712-3

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Desensitization of cold- and menthol-sensitive rat dorsal root ganglion neurones by inflammatory mediators

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