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Erschienen in: Calcified Tissue International 6/2016

18.02.2016 | Original Research

Systemic Inflammation Affects Human Osteocyte-Specific Protein and Cytokine Expression

verfasst von: Janak L. Pathak, Astrid D. Bakker, Frank P. Luyten, Patrick Verschueren, Willem F. Lems, Jenneke Klein-Nulend, Nathalie Bravenboer

Erschienen in: Calcified Tissue International | Ausgabe 6/2016

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Abstract

Bone remodeling can be disturbed in active rheumatoid arthritis (RA), possibly as a result of elevated levels of circulating inflammatory cytokines. Osteocyte-specific proteins and cytokines play a vital role in bone remodeling by orchestrating bone formation and/or bone resorption. Therefore, we aimed to investigate the effect of RA-serum or inflammatory cytokines on expression of human osteocyte-specific proteins and cytokines. Human trabecular bone chips were cultured with RA-serum or inflammatory cytokines for 7-days. Live-dead staining was performed to assess cell viability. Gene expression of osteocyte-specific proteins and cytokines was analyzed by qPCR. Immuno-staining was performed for osteocyte-specific markers. Approximately 60 % of the osteocytes on the bone chips were alive at day-7. Cells in or on the bone chips did express the gene for osteocyte markers SOST, FGF23, DMP1, and MEPE, and the cytokines IL-, IL-6, and TNFα at day 0 and 7. Active RA-serum treatment enhanced IL-1β, TNFα, SOST, and DKK1 gene expression. IL-1β treatment enhanced IL-1β, TNFα, IL-6, IL-8, FGF23, and SOST gene expression. TNFα treatment enhanced IL-1β, TNFα, IL-6, IL-8, and FGF23 gene expression. IL-8 treatment enhanced TNFα, IL-8, and FGF23 gene expression. A combination of IL-1β, IL-6, and TNFα treatment synergistically upregulated IL-1β, IL-6, and IL-8 gene expression, as well as enhanced TNFα, OPG, SOST, and FGF23, and inhibited DKK1 gene expression. In conclusion, gene expression of human osteocyte-specific proteins and cytokines was affected by RA-serum, and exogenous recombinant cytokines treatment suggesting that osteocytes could provide a new target to prevent systemic inflammation-induced bone loss in RA.
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Metadaten
Titel
Systemic Inflammation Affects Human Osteocyte-Specific Protein and Cytokine Expression
verfasst von
Janak L. Pathak
Astrid D. Bakker
Frank P. Luyten
Patrick Verschueren
Willem F. Lems
Jenneke Klein-Nulend
Nathalie Bravenboer
Publikationsdatum
18.02.2016
Verlag
Springer US
Erschienen in
Calcified Tissue International / Ausgabe 6/2016
Print ISSN: 0171-967X
Elektronische ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-016-0116-8

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