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DNA-Binding, Photocleavage, and Photodynamic Anti-cancer Activities of Pyridyl Corroles

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Abstract

The DNA-binding, photocleavage, and antitumor activity of three free base pyridyl corroles 1, 2, and 3 have been investigated. The binding affinity toward CT-DNA decreases with increasing number of pentafluorophenyl, whereas the photocleavage activity toward pBR322 DNA becomes more efficient. Singlet oxygen was demonstrated as active species responsible for DNA cleavage. These corroles exhibited high cytotoxicity against three tested cancer cells (Hela, HapG2, and A549) and the cytotoxicity could be further enhanced under irradiation. Intracellular reactive oxygen species level was also monitored using HeLa Cells upon the combined treatment of corroles and light. These corroles could be absorbed by HeLa cells at low concentration. They can induce the decrease of mitochondrial membrane potential and apoptosis of tumor cells under irradiation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (NNSFC) under Grant (Nos: 21371059, 21376099), the High-Level Personnel Project of Guangdong Province in 2013, and the Joint Natural Science Fund of the Department of Science and Technology and the First Affiliated Hospital of Guangdong Pharmaceutical University (No. GYFYLH201315).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510641, People’s Republic of China

    Zhen-Hua Liang, Hai-Yang Liu, Rong Zhou, Zao Zhang, Atif Ali & Xin-Yan Xiao

  2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Bing-Jie Han & Yun-Jun Liu

  3. Department of Chemistry and Bioengineering, Yichun University, Yichun, 336000, People’s Republic of China

    Rong Zhou

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  1. Zhen-Hua Liang
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Correspondence to Hai-Yang Liu, Yun-Jun Liu or Xin-Yan Xiao.

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Liang, ZH., Liu, HY., Zhou, R. et al. DNA-Binding, Photocleavage, and Photodynamic Anti-cancer Activities of Pyridyl Corroles. J Membrane Biol 249, 419–428 (2016). https://doi.org/10.1007/s00232-016-9879-0

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  • DOI: https://doi.org/10.1007/s00232-016-9879-0

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