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Nigerian Bonny Light Crude Oil Disrupts Antioxidant Systems in Testes and Sperm of Rats

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Abstract

Nigerian Bonny light crude oil (BLCO) is commonly used by the local population in folklore medicine for the management of various forms of gastrointestinal problems and male reproductive capacity. The study investigated the effects of BLCO on the antioxidant systems of the testes and epidydimal sperm in rats by oral exposure to 0, 200, 400 and 800 mg/kg BLCO for 7 days. In testes and sperm, BLCO treatment at all doses significantly (p < 0.05) decreased superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities, whereas it markedly increased glucose 6-phosphate dehydrogenase (G6PD) and gamma glutamyl transferase (GGT) activities as well as increased glutathione (GSH), hydrogen peroxide, and malondialdehyde (MDA) levels in all treatment groups. Although epididymal sperm number (ESN), daily spermatozoa production (DSP), and sperm motility were significantly decreased, total sperm abnormalities were significantly increased without affecting sperm viability at all dose levels compared with controls. The adverse effect of BLCO on TSN was noted at the 800 mg/kg dose only. Histopathology results showed treatment-related lesions of the testes characterized by severe congestion of interstitial vessels, decreased germinal epithelium, and increased number of vacuolization. These results suggest that exposure to BLCO, such as its use in ailment management, may promote infertility by altering the function of the testes and sperm, particularly by way of induction of oxidative stress.

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References

  • Abarikwu SO, Adesiyan AC, Oyejola TO, Oyeyemi MO, Farombi EO (2009) Changes in sperm characteristics and induction of oxidative stress in the testis and epididymis of experimental rats by a herbicide, atrazine. Arch Environ Contam Toxicol. doi:10.1007/s00244-009-9371-2

  • Aitken RJ, Buckingham D, Harkiss D (1993) Relationship between iron-catalyzed lipid peroxidation potential and human sperm function. J Reprod Fertil 98:257–265

    CAS  Google Scholar 

  • Aitkin RJ, Clarkson JS, Fishel S (1989) Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biol Reprod 41:183–187

    Article  Google Scholar 

  • Akintonwa A, Ebere AG (1990) Toxicity of Nigerian crude oil and chemical dispersants to Barbus Sp. and Clarias Sp. Bull Environ Contam Toxicol 45:729–733

    Article  CAS  Google Scholar 

  • Alvarez JG, Touchstone JC, Blasco L, Storey B (1987) Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. J Androl 8:338–348

    CAS  Google Scholar 

  • Amstad P, Peskin A, Shah AG, Mirault ME, Moret R, Zbinden I et al (1991) The balance between Cu, Zn-superoxide dismutase and catalase affects the sensitivity of mouse epidermal cells to oxidative stress. Biochemistry 30:9305–9313

    Article  CAS  Google Scholar 

  • Bauche F, Fouchard H, Jegou B (1994) Antioxidant system in rat testicular cells. FEBS Lett 349:392–396

    Article  CAS  Google Scholar 

  • Carpenter DO, Arcaro K, Spink DC (2002) Understanding the human health effects of chemical mixtures. Environ Health Perspect 110:25–42

    CAS  Google Scholar 

  • Clairborne A (1995) Catalase activity. In: Greewald AR (ed) Handbook of methods for oxygen radical research. CRC Press, Boca Raton, FL, pp 237–242

    Google Scholar 

  • CONCAWE (2001) Environmental classification of petroleum substances—summary data and rationale. Report No. 01/54. CONCAWE, Brussels

    Google Scholar 

  • Dawson JP, Thayer WW, Desforges JF (1958) Acute hemolytic anaemia in the newborn infant due to naphthalene poisoning: report of two cases with investigations into the mechanism of the disease. Hematology 13:1113–1125

    CAS  Google Scholar 

  • Dickinson DA, Moellering DR, Iles KE, Patel RP, Levonen AL, Wigley A et al (2003) Cytoprotection against oxidative stress and the regulation of glutathione synthesis. Biol Chem 384:527–537

    Article  CAS  Google Scholar 

  • Dimitrova MST, Tsinova V, Velcheva V (1994) Combined effect of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (cyprinus carpio). Comp Biochem Physiol 108:43–46

    Google Scholar 

  • Farombi EO, Tahnteng JG, Agboola AO, Nwankwo JO, Emerole GO (2000) Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron-a garcinia kola seed extract. Food Chem Toxicol 38:353–541

    Google Scholar 

  • Farombi EO, Abarikwu SO, Adedara IA, Oyeyemi MO (2007) Curcumin and Kolaviron ameliorate Di-n-butylphthalate-induced testicular damage in rats. Basic Clin Pharmacol Toxicol 100:43–48

    Article  CAS  Google Scholar 

  • Farombi EO, Ugwuezunmba MC, Ezenwadu TT, Oyeyemi MO, Ekor M (2008) Tetracycline-induced reproductive toxicity in male rats: Effects of vitamin C and N-acetylcysteine. Exp Toxicol Pathol 60:77–85

    Article  CAS  Google Scholar 

  • Fraga CG, Motchnik PA, Shigenaga MK, Helbock HJ, Jacob RA, Ames BN (1991) Ascorbic acid protects against endogenous oxidative damage in human sperm. Proc Natl Acad Sci USA 88:11003–11006

    Article  CAS  Google Scholar 

  • Franca LR, Leal MC, Sasso-Cerri E, Vasconcelos A, Debeljuk L, Russell LD (2000) Cimetidine (Tagamet) is a reproductive toxicant in male rats affecting peritubular cells. Biol Reprod 63:1403–1412

    Article  CAS  Google Scholar 

  • Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112

    Article  CAS  Google Scholar 

  • Girotti AW (1985) Mechanisms of lipid peroxidation. J Free Radic Biol Med 1:87–95

    Article  CAS  Google Scholar 

  • Giwa-Osagie OO (2003) Nigeria has twelve million infertile persons. Pharmanews 25:48–49

    Google Scholar 

  • Griendling KK, Sorescu D, Ushio-Fukai M (2000) NADPH oxidase: role in cardiovascular biology and disease. Circ Res 86:494–501

    CAS  Google Scholar 

  • Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    CAS  Google Scholar 

  • Hasspielar BM, Behar JV, DiGiulio RT (1994) Glutathione dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameiurus nebulosus). Ecotoxicol Environ Saf 28:82–90

    Article  Google Scholar 

  • Iwasaki A, Gagnon C (1992) Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril 57:409–416

    CAS  Google Scholar 

  • Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR (1974) Bromobenzene induced liver necrosis: Protective role of glutathione and evidence for 3,4 bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11:151–169

    Article  CAS  Google Scholar 

  • Joyce KL, Porcelli J, Cooke PS (1993) Neonatal goitrogen treatment increases adult testes size and sperm production in the mouse. J Androl 14:448–455

    CAS  Google Scholar 

  • Kumar S (2008) Is environmental exposure associated with reproductive health impairments? J Turk Ger Gynecol Assoc 9:60–69

    Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Lucesoli F, Caligiuri M, Roberti MF, Perazzo JC, Fraga CG (1999) Dose-dependent increase of oxidative damage in the testis of rats subjected to acute iron overload. Arch Biochem Biophys 372:37–43

    Article  CAS  Google Scholar 

  • Markelewicz RJ, Hall SJ, Boekeldeide K (2004) 2,5-hexanedione and carbendazim coexposure synergistically disrupts rats spermatogenesis despite opposing molecular effects on microtubules. Toxicol Sci 80:92–100

    Article  CAS  Google Scholar 

  • McLachlan R, de Krester D (2001) Male infertility: the case for continued research. Med J Aust 174:116–117

    CAS  Google Scholar 

  • Misra HP, Fridovich I (1972) The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175

    CAS  Google Scholar 

  • Oehninger S (2001) Strategies for the infertile man. Semin Reprod Med 19:231–237

    Article  CAS  Google Scholar 

  • Orisakwe OE, Njan AA, Afonne JO, Akumka DD, Orish N, Udemezue OO (2004a) Investigation into the nephrotoxicity of Nigerian bonny light crude oil in albino rats. Int J Environ Res Public Health 1:106–110

    Article  CAS  Google Scholar 

  • Orisakwe OE, Akumka DD, Njan AA, Afonne JO (2004b) Testicular toxicity of Nigerian bonny light crude oil in male albino rats. Reprod Toxicol 18:439–442

    Article  CAS  Google Scholar 

  • Oruambo IF, Jones AB (2007) Alterations in the concentrations of liver mitochondrial DNA: cytoplasmic total hydrocarbon and calcium in guinea pigs after treatment with Nigerian light crude oil. Int J Environ Res Public Health 4:23–27

    Article  CAS  Google Scholar 

  • Oruambo IF, Kachikwu S, Idabor L (2007) Dose-related increased binding of nickel to chromatin proteins and changes to DNA concentration in the liver of guinea pigs treated with Nigerian light crude oil. Int J Environ Res Public Health 4:211–215

    Article  CAS  Google Scholar 

  • Pant N, Srivastava SP (2003) Testicular and spermatotoxic effects of quinalphos in rats. J Appl Toxicol 23:271–274

    Article  CAS  Google Scholar 

  • Pant N, Prasad AK, Srivastava SC, Shanker R, Srivastava SP (1995) Effect of oral administration of carbofuran on reproductive system of rat. Hum Exp Toxicol 14:889–894

    Article  CAS  Google Scholar 

  • Perreault SD (1998) Gamete toxicology: the impact of new technologies. In: Korach KS (ed) Reproductive and developmental toxicology. Marcel Dekker, New York, NY, pp 635–654

    Google Scholar 

  • Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ et al (2002) Best practice policies for male infertility. Fertil Steril 77:873–882

    Article  Google Scholar 

  • Stephensen E, Sturve J, Forlin L (2002) Effect of redox cycling compounds on glutathione content and activity of glutathione-related enzymes in rainbow trout liver. Comp Biochem Physiol C 133:435–442

    Google Scholar 

  • Stohs SJ, Bagchi D (1995) Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 18:321–336

    Article  CAS  Google Scholar 

  • Szasz G (1974) Gamma glutamyltranspeptidase. In: Bergmeye A (ed) Methods of enzymatic analysis, 2nd edn. Academic Press, New York, NY, p 715

    Google Scholar 

  • Teuschler L, Klauring J, Carney E, Chambers J, Conolly R, Gennings C et al (2002) Support of the science-based decisions concerning the evaluation of the toxicology of mixtures: a new beginning. Regul Toxicol Pharmacol 36:34–39

    Article  Google Scholar 

  • Wells ME, Awa OA (1970) New technique for assessing acrosomal characteristics of spermatozoa. J Dairy Sci 53:227

    Article  CAS  Google Scholar 

  • Wolff SP (1994) Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol 233:182 (Academic Press 189)

    Google Scholar 

  • Zemjanis R (1970) Collection and evaluation of semen. In: Diagnostic and therapeutic technique in animal reproduction, 2nd ed. William and Wilkins, Baltimore, MD, pp 139–153

Download references

Acknowledgments

The authors gratefully acknowledge the technical assistance of Omoko Ejiro of the Department of Veterinary Surgery and Reproduction, University of Ibadan. This work was supported by Senate Research Grant Code No. SRG/Com/2006/34A from the University of Ibadan, Nigeria (E. O. F.).

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  1. Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria

    Ebenezer O. Farombi, Isaac A. Adedara, Azubike P. Ebokaiwe, Roy Teberen & Theresa Ehwerhemuepha

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  1. Ebenezer O. Farombi
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  2. Isaac A. Adedara
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Correspondence to Ebenezer O. Farombi.

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Farombi, E.O., Adedara, I.A., Ebokaiwe, A.P. et al. Nigerian Bonny Light Crude Oil Disrupts Antioxidant Systems in Testes and Sperm of Rats. Arch Environ Contam Toxicol 59, 166–174 (2010). https://doi.org/10.1007/s00244-009-9443-3

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