Abstract
A CD40 homolog cDNA encoding 300 amino acid residues was isolated from a Japanese flounder leukocyte cDNA library. The amino acid sequence identities of Japanese flounder CD40 and previously reported CD40s of cow, human, mouse, and chicken range from 32 to 35%. The positions of cysteine residues, CD40 ligand binding amino acid residues, and four cysteine-rich domains are well conserved in Japanese flounder CD40. The Japanese flounder CD40 gene is composed of nine exons and eight intervening introns spread over 6 kb. The nucleotide sequence of the 5′-flanking region of this gene revealed the presence of several regulatory regions, including a TATA-like box, AP-1-, CEBPB-, IRF-1-, LYF-1-, NF-κBp-, SP-1-, and Stat-1-like motifs. In healthy fish, reverse transcriptase–polymerase chain reaction (RT-PCR) detected constitutive expression of CD40 in all tested tissues (leukocytes, kidney, spleen, liver, intestine, brain, gill, and skin). The mRNA of CD40 was predominantly expressed in several tissues that contained lymphocytes (leukocytes, kidney, spleen, intestine, and gill). Expression of the Japanese flounder CD40 molecule was induced in peripheral blood leukocytes from 1 to 6 h following Con A (50 μg/ml)/phorbol myristate acetate (PMA) (0.35 μg/ml) stimulation, with a peak at 1 h after stimulation, and increased at 1, 3, and 6 h after induction by lipopolysaccharide (LPS) (500 μg/ml) compared with the control.
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References
Altschul SF, Gish W, Miller W, Myers EW, Lipman EW (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Altschul SF, Madden TL, Schofer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Aoki T, Nam B-H, Hirono I (1999) Sequence of 596 cDNA clones (565,977) of Japanese flounder Paralichthys olivaceus leukocytes infected with hirame rhabdovirus. Mar Biotechnol 1:477–488
Bajorath J, Chalupny NJ, Marken JS, Siadak AW, Skonier J, Gordon M, Hollenbaugh D, Noelle RJ, Ochs HD, Aruffo A (1995) Identification of residues on CD40 and its ligand which are critical for the receptor–ligand interaction. Biochemistry 34:1833–1844
Braesch-Andersen S, Paulie S, Koho H, Nika H, Aspenstrom P, Perlmann P (1989) Biochemical characteristics and partial amino acid sequence of the receptor-like human B cell and carcinoma antigen CDw40. J Immunol 142:562–567
Clark EA, Ledbetter JA (1986) Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50. Proc Natl Acad Sci U S A 83:4494–4498
Felsenstein J (1996) PHYLIP (phylogeny inference package), version 4.0. Department of Genetics, University of Washington, Seattle
Fries KM, Sempowski GD, Gaspari AA, Blieden T, Looney RJ, Phipps RP (1995) CD40 expression by human fibroblasts. Clin Immunol Immunopathol 77:42–51
Galy AH, Spits H (1992) CD40 is functionally expressed on human thymic epithelial cells. J Immunol 149:775–782
Graham S, Secombes CJ (1988) The production of a macrophage-activating factor from rainbow trout Salmo gairdneri leucocytes. Immunology 65:293–297
Hart DN, McKenzie JL (1988) Isolation and characterization of human tonsil dendritic cells. J Exp Med 168:157–170
Hirono I, Nam B-H, Kurobe T, Aoki T (2000) Molecular cloning, characterization, and expression of TNF cDNA and gene from Japanese flounder Paralichthys olivaceus. J Immunol 165:4423–4427
Johnson MD, Housey GM, Kirschmeier PT, Weinstein IB (1987) Molecular cloning of gene sequences regulated by tumor promoters and mitogens through protein kinase C. Mol Cell Biol 7:2821–2829
Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS (1995) CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proc Natl Acad Sci U S A 92:4342–4346
Katagiri T, Asakawa S, Hirono I, Aoki T, Shimizu N (2000) Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus. Mar Biotechnol 2:571–576
Kooten C, Banchereau J (2000) CD40-CD40 ligand. J Leukoc Biol 67:2–17
Krzesz R, Wagner AH, Cattaruzza M, Hecker M (1999) Cytokine-inducible CD40 gene expression in vascular smooth muscle cells is mediated by nuclear factor κB and signal transducer and activator of transcription-1. FEBS Lett 453:191–196
Murphy JJ, Norton JD (1993) Phorbol ester induction of early response gene expression in lymphocytic leukemia and normal human B cells. Leuk Res 17:657–662
Nam B-H, Yamamoto E, Hirono I, Aoki T (2000) A survey of expressed genes in the leukocytes of Japanese flounder, Paralichthys olivaceus, infected with hirame rhabdovirus. Dev Comp Immunol 24:13–24
Ota N, Nakajima T, Takeuchi T, Shirai Y, Emi M (1999) A highly polymorphic CA repeat marker at the interleukin-11 locus. Genes Immun 1:159–160
Park C-I, Kurobe T, Hirono I, Aoki T (2003) Cloning and characterization of cDNA for two distinct tumor necrosis factor receptor genes from Japanese flounder Paralichthys olivaceus. Dev Comp Immunol 27:365–375
Ridge JP, Rosa F, Matzinger P (1998) A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell. Nature 393:474–478
Schönbeck U, Libby P (2001) The CD40/CD15 receptor/ligand dyad. Cell Mol Life Sci 58:4–43
Schönbeck U, Mach M, Bonnefoy JY, Loppnow H, Flad HD, Libby P (1997) Ligation of CD40 activates interleukin 1β-converting enzyme (caspase-1) activity in vascular smooth muscle and endothelial cells and promotes elaboration of active interleukin 1β. J Biol Chem 272:19569–19574
Sempowski GD, Chess PR, Moretti AJ, Padilla J, Phipps RP, Blieden TM (1997) CD40 mediated activation of gingival and periodontal ligament fibroblasts. J Periodontol 68:284–292
Stamenkovic I, Clark EA, Seed B (1989) A B lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J 8:1403–1410
Takeshita T, Goto Y, Nakamura M, Fujii M, Iwami M, Hinuma Y, Sugamura K (1988) Phorbol esters can persistently replace interleukin-2 (IL-2) for the growth of a human IL-2-dependent T-cell line. J Cell Physiol 136:319–325
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This study was supported in part by grants-in-aid for Scientific Research (S) (no.15108003) from the Ministry of Education, Science, Sports and Culture of Japan, and a research grant of the JSPS.
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The nucleotide sequence data reported in this paper have been submitted to the DDBJ nucleotide database and have been assigned the accession number BAC87848 (CD40 cDNA)
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Park, CI., Hirono, I., Hwang, J.Y. et al. Characterization and expression of a CD40 homolog gene in Japanese flounder Paralichthys olivaceus . Immunogenetics 57, 682–689 (2005). https://doi.org/10.1007/s00251-005-0032-y
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DOI: https://doi.org/10.1007/s00251-005-0032-y