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European Journal of Nuclear Medicine and Molecular Imaging
Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging 5/2007

01.05.2007 | Original article

Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells

verfasst von: Bernd Nowak, Christian Weber, Andreas Schober, Ute Zeiffer, Elisa A. Liehn, Philipp von Hundelshausen, Patrick Reinartz, Wolfgang M. Schaefer, Ulrich Buell

Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging | Ausgabe 5/2007

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Abstract

Purpose

Cell-based therapy by transplantation of progenitor cells has emerged as a promising development for organ repair, but non-invasive imaging approaches are required to monitor the fate of transplanted cells. Radioactive labelling with 111In-oxine has been used in preclinical trials. This study aimed to validate 111In-oxine labelling and subsequent in vivo and ex vivo detection of haematopoietic progenitor cells.

Methods

Murine haematopoietic progenitor cells (106, FDCPmix) were labelled with 0.1 MBq (low dose) or 1.0 MBq (high dose) 111In-oxine and compared with unlabelled controls. Cellular retention of 111In, viability and proliferation were determined up to 48 h after labelling. Labelled cells were injected into the cavity of the left or right cardiac ventricle in mice. Scintigraphic images were acquired 24 h later. Organ samples were harvested to determine the tissue-specific activity.

Results

Labelling efficiency was 75±14%. Cellular retention of incorporated 111In after 48 h was 18±4%. Percentage viability after 48 h was 90±1% (control), 58±7% (low dose) and 48±8% (high dose) (p<0.0001). Numbers of viable cells after 48 h (normalised to 0 h) were 249±51% (control), 42±8% (low dose) and 32±5% (high dose) (p<0.0001). Cells accumulated in the spleen (86.6±27.0% ID/g), bone marrow (59.1±16.1% ID/g) and liver (30.3±9.5% ID/g) after left ventricular injection, whereas most of the cells were detected in the lungs (42.4±21.8% ID/g) after right ventricular injection.

Conclusion

Radiolabelling of haematopoietic progenitor cells with 111In-oxine is feasible, with high labelling efficiency but restricted stability. The integrity of labelled cells is significantly affected, with substantially reduced viability and proliferation and limited migration after systemic transfusion.
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Metadaten
Titel
Indium-111 oxine labelling affects the cellular integrity of haematopoietic progenitor cells
verfasst von
Bernd Nowak
Christian Weber
Andreas Schober
Ute Zeiffer
Elisa A. Liehn
Philipp von Hundelshausen
Patrick Reinartz
Wolfgang M. Schaefer
Ulrich Buell
Publikationsdatum
01.05.2007
Verlag
Springer Berlin Heidelberg
Erschienen in
European Journal of Nuclear Medicine and Molecular Imaging / Ausgabe 5/2007
Print ISSN: 1619-7070
Elektronische ISSN: 1619-7089
DOI
https://doi.org/10.1007/s00259-006-0275-3

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