Erschienen in:
01.12.2008 | Original Article
Dynamic 18F-fluoride small animal PET to noninvasively assess renal function in rats
verfasst von:
Uta Schnöckel, Stefan Reuter, Lars Stegger, Eberhard Schlatter, Klaus P. Schäfers, Sven Hermann, Otmar Schober, Gert Gabriëls, Michael Schäfers
Erschienen in:
European Journal of Nuclear Medicine and Molecular Imaging
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Ausgabe 12/2008
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Abstract
Purpose
Renal function can be quantified by both laboratory and scintigraphic methods. In the case of small animal diagnostics, scintigraphic image-based methods are ideal since they can assess split renal function, work noninvasively, and can be repeated. The aim of this study is to validate a 18F-PET-based method to quantify renal function in rats.
Materials and methods
Fluoride clearance was calculated from a dynamic whole body listmode acquisition of 60 min length in a small animal PET scanner following an i.v. injection of 15 MBq 18F-fluoride. Volumes of interest (VOIs) were placed in the left ventricle and the bladder as well as traced around the kidney contours. The respective time–activity curves (TAC) were calculated. The renal 18F-clearance was calculated by the ratio of the total renal excreted activity (bladder VOI) and the integral of the blood TAC. PET-derived renal function was validated by intraindividual measurements of creatinine clearance (n = 23), urea clearance (n = 23), and tubular excretion rate (TER-MAG3). The split renal function was derived from the injection of the clinically available radionuclide 99mTc-mercaptotriglycine by blood sampling and planar renography (n = 8).
Results
In all animals studied, PET revealed high-quality TACs. PET-derived renal fluoride clearance was linearly correlated with intraindividual laboratory measures (PET vs. creatinine: r = 0.78; PET vs. urea: r = 0.73; PET vs. TER-MAG3: r = 0.73). Split function was comparable (18F-PET vs. MAG3-renography: r = 0.98). PET-derived measures were highly reproducible.
Conclusions
18F-PET is able to noninvasively assess renal function in rats and provides a significant potential for serial studies in different experimental scenarios.