Uptake of inflammatory cell marker [11C]PK11195 into mouse atherosclerotic plaques | springermedizin.de

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European Journal of Nuclear Medicine and Molecular Imaging

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01.01.2009 | Original Article

Uptake of inflammatory cell marker [¹¹C]PK11195 into mouse atherosclerotic plaques

verfasst von: Iina Laitinen, Päivi Marjamäki, Kjell Någren, V. Jukka O. Laine, Ian Wilson, Pia Leppänen, Seppo Ylä-Herttuala, Anne Roivainen, Juhani Knuuti

Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging | Ausgabe 1/2009

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Abstract

Purpose

The ligand [¹¹C]PK11195 binds with high affinity and selectivity to peripheral benzodiazepine receptor, expressed in high amounts in macrophages. In humans, [¹¹C]PK11195 has been used successfully for the in vivo imaging of inflammatory processes of brain tissue. The purpose of this study was to explore the feasibility of [¹¹C]PK11195 in imaging inflammation in the atherosclerotic plaques.

Methods

The presence of PK11195 binding sites in the atherosclerotic plaques was verified by examining the in vitro binding of [³H]PK11195 onto mouse aortic sections. Uptake of intravenously administered [¹¹C]PK11195 was studied ex vivo in excised tissue samples and aortic sections of a LDLR/ApoB48 atherosclerotic mice. Accumulation of the tracer was compared between the atherosclerotic plaques and non-atherosclerotic arterial sites by autoradiography and histological analyses.

Results

The [³H]PK11195 was found to bind to both the atherosclerotic plaques and the healthy wall. The autoradiography analysis revealed that the uptake of [¹¹C]PK11195 to inflamed regions in plaques was more prominent (p = 0.011) than to non-inflamed plaque regions, but overall it was not higher than the uptake to the healthy vessel wall. Also, the accumulation of ¹¹C radioactivity into the aorta of the atherosclerotic mice was not increased compared to the healthy control mice.

Conclusions

Our results indicate that the uptake of [¹¹C]PK11195 is higher in inflamed atherosclerotic plaques containing a large number of inflammatory cells than in the non-inflamed plaques. However, the tracer uptake to other structures of the artery wall was also prominent and may limit the use of [¹¹C]PK11195 in clinical imaging of atherosclerotic plaques.

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Titel: Uptake of inflammatory cell marker [11C]PK11195 into mouse atherosclerotic plaques
verfasst von: Iina Laitinen
Päivi Marjamäki
Kjell Någren
V. Jukka O. Laine
Ian Wilson
Pia Leppänen
Seppo Ylä-Herttuala
Anne Roivainen
Juhani Knuuti
Publikationsdatum: 01.01.2009
Verlag: Springer-Verlag
Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging / Ausgabe 1/2009
Print ISSN: 1619-7070
Elektronische ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-008-0919-6