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01.10.2009 | Original Article

Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor β

verfasst von: Taku Nagai, Masashi Tanaka, Yasuhiro Tsuneyoshi, Baohui Xu, Sara A. Michie, Kazuhisa Hasui, Hirofumi Hirano, Kazunori Arita, Takami Matsuyama

Erschienen in: Cancer Immunology, Immunotherapy | Ausgabe 10/2009

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Abstract

Tumor-associated macrophages (TAMs) are frequently found in glioblastomas and a high degree of macrophage infiltration is associated with a poor prognosis for glioblastoma patients. However, it is unclear whether TAMs in glioblastomas promote tumor growth. In this study, we found that folate receptor β (FRβ) was expressed on macrophages in human glioblastomas and a rat C6 glioma implanted subcutaneously in nude mice. To target FRβ-expressing TAMs, we produced a recombinant immunotoxin consisting of immunoglobulin heavy and light chain Fv portions of an anti-mouse FRβ monoclonal antibody and Pseudomonas exotoxin A. Injection of the immunotoxin into C6 glioma xenografts in nude mice significantly depleted TAMs and reduced tumor growth. The immunotoxin targeting FRβ-expressing macrophages will provide a therapeutic tool for human glioblastomas.

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Titel: Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor β
verfasst von: Taku Nagai
Masashi Tanaka
Yasuhiro Tsuneyoshi
Baohui Xu
Sara A. Michie
Kazuhisa Hasui
Hirofumi Hirano
Kazunori Arita
Takami Matsuyama
Publikationsdatum: 01.10.2009
Verlag: Springer-Verlag
Erschienen in: Cancer Immunology, Immunotherapy / Ausgabe 10/2009
Print ISSN: 0340-7004
Elektronische ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-009-0667-x

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Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor β

Abstract

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Springer Medizin

Abstract

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