nach oben

Erschienen in:

21.05.2018 | Symposium-in-Writing Paper

How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions

verfasst von: Annika M. Bruger, Anca Dorhoi, Gunes Esendagli, Katarzyna Barczyk-Kahlert, Pierre van der Bruggen, Marie Lipoldova, Tomas Perecko, Juan Santibanez, Margarida Saraiva, Jo A. Van Ginderachter, Sven Brandau

Erschienen in: Cancer Immunology, Immunotherapy | Ausgabe 4/2019

Einloggen, um Zugang zu erhalten

Abstract

Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.

Pradeu T, Cooper EL (2012) The danger theory: 20 years later. Front Immunol 3:287. https://doi.org/10.3389/fimmu.2012.00287 CrossRefPubMedPubMedCentral

Libby P (2007) Inflammatory mechanisms: the molecular basis of inflammation and disease. Nutr Rev 65:S140-6CrossRefPubMed

Iqbal AJ, Fisher EA, Greaves DR (2016) Inflammation-a critical appreciation of the role of myeloid cells. Microbiol Spectr. https://doi.org/10.1128/microbiolspec.MCHD-0027-2016 CrossRefPubMed

Gabrilovich DI (2017) Myeloid-derived suppressor cells. Cancer Immunol Res 5:3–8. https://doi.org/10.1158/2326-6066.CIR-16-0297 CrossRefPubMedPubMedCentral

Bronte V, Brandau S, Chen S-H, Colombo MP, Frey AB, Greten TF, Mandruzzato S, Murray PJ, Ochoa A, Ostrand-Rosenberg S, Rodriguez PC, Sica A, Umansky V, Vonderheide RH, Gabrilovich DI (2016) Recommendations for myeloid-derived suppressor cell nomenclature and characterization standards. Nat Commun 7:12150. https://doi.org/10.1038/ncomms12150 CrossRefPubMedPubMedCentral

Haile LA, Greten TF, Korangy F (2012) Immune suppression: the hallmark of myeloid derived suppressor cells. Immunol Invest 41:581–594. https://doi.org/10.3109/08820139.2012.680635 CrossRefPubMedPubMedCentral

Weber J, Gibney G, Kudchadkar R, Yu B, Cheng P, Martinez AJ, Kroeger J, Richards A, McCormick L, Moberg V, Cronin H, Zhao X, Schell M, Chen YA (2016) Phase I/II Study of metastatic melanoma patients treated with nivolumab who had progressed after ipilimumab. Cancer Immunol Res 4:345–353. https://doi.org/10.1158/2326-6066.CIR-15-0193 CrossRefPubMedPubMedCentral

de Coana YP, Wolodarski M, Poschke I, Yoshimoto Y, Yang Y, Nystrom M, Edback U, Brage SE, Lundqvist A, Masucci GV, Hansson J, Kiessling R (2017) Ipilimumab treatment decreases monocytic MDSCs and increases CD8 effector memory T cells in long-term survivors with advanced melanoma. Oncotarget 8:21539–21553. https://doi.org/10.18632/oncotarget.15368 CrossRefPubMedPubMedCentral

Chesney JA, Mitchell RA, Yaddanapudi K (2017) Myeloid-derived suppressor cells-a new therapeutic target to overcome resistance to cancer immunotherapy. J Leukoc Biol 102:727–740. https://doi.org/10.1189/jlb.5VMR1116-458RRR CrossRefPubMedPubMedCentral

10.

Monu NR, Frey AB (2012) Myeloid-derived suppressor cells and anti-tumor T cells: a complex relationship. Immunol Invest 41:595–613. https://doi.org/10.3109/08820139.2012.673191 CrossRefPubMedPubMedCentral

11.

Srivastava MK, Sinha P, Clements VK, Rodriguez P, Ostrand-Rosenberg S (2010) Myeloid-derived suppressor cells inhibit T-cell activation by depleting cystine and cysteine. Cancer Res 70:68–77. https://doi.org/10.1158/0008-5472.CAN-09-2587 CrossRefPubMed

12.

Raber PL, Thevenot P, Sierra R, Wyczechowska D, Halle D, Ramirez ME, Ochoa AC, Fletcher M, Velasco C, Wilk A, Reiss K, Rodriguez PC (2014) Subpopulations of myeloid-derived suppressor cells impair T cell responses through independent nitric oxide-related pathways. Int J Cancer 134:2853–2864. https://doi.org/10.1002/ijc.28622 CrossRefPubMed

13.

Youn J-I, Collazo M, Shalova IN, Biswas SK, Gabrilovich DI (2012) Characterization of the nature of granulocytic myeloid-derived suppressor cells in tumor-bearing mice. J Leukoc Biol 91:167–181. https://doi.org/10.1189/jlb.0311177 CrossRefPubMedPubMedCentral

14.

Liu C, Yu S, Kappes J, Wang J, Grizzle WE, Zinn KR, Zhang H-G (2007) Expansion of spleen myeloid suppressor cells represses NK cell cytotoxicity in tumor-bearing host. Blood 109:4336–4342. https://doi.org/10.1182/blood-2006-09-046201 CrossRefPubMedPubMedCentral

15.

Knaul JK, Jörg S, Oberbeck-Mueller D, Heinemann E, Scheuermann L, Brinkmann V, Mollenkopf H-J, Yeremeev V, Kaufmann SHE, Dorhoi A (2014) Lung-residing myeloid-derived suppressors display dual functionality in murine pulmonary tuberculosis. Am J Respir Crit Care Med 190:1053–1066. https://doi.org/10.1164/rccm.201405-0828OC CrossRefPubMed

16.

Li H, Han Y, Guo Q, Zhang M, Cao X (2009) Cancer-expanded myeloid-derived suppressor cells induce anergy of NK cells through membrane-bound TGF-beta 1. J Immunol 182:240–249CrossRefPubMed

17.

Rieber N, Singh A, Öz H, Carevic M, Bouzani M, Amich J, Ost M, Ye Z, Ballbach M, Schäfer I, Mezger M, Klimosch SN, Weber ANR, Handgretinger R, Krappmann S, Liese J, Engeholm M, Schüle R, Salih HR et al (2015) Pathogenic fungi regulate immunity by inducing neutrophilic myeloid-derived suppressor cells. Cell Host Microbe 17:507–514. https://doi.org/10.1016/j.chom.2015.02.007 CrossRefPubMedPubMedCentral

18.

Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system. Nat Rev Immunol 9:162–174. https://doi.org/10.1038/nri2506 CrossRefPubMedPubMedCentral

19.

Kusmartsev S, Nefedova Y, Yoder D, Gabrilovich DI (2004) Antigen-specific inhibition of CD8+ T cell response by immature myeloid cells in cancer is mediated by reactive oxygen species. J Immunol 172:989–999CrossRefPubMed

20.

Nagaraj S, Gupta K, Pisarev V, Kinarsky L, Sherman S, Kang L, Herber DL, Schneck J, Gabrilovich DI (2007) Altered recognition of antigen is a mechanism of CD8+ T cell tolerance in cancer. Nat Med 13:828–835. https://doi.org/10.1038/nm1609 CrossRefPubMedPubMedCentral

21.

Youn J-I, Nagaraj S, Collazo M, Gabrilovich DI (2008) Subsets of myeloid-derived suppressor cells in tumor-bearing mice. J Immunol 181:5791–5802CrossRefPubMed

22.

Huang B, Pan P-Y, Li Q, Sato AI, Levy DE, Bromberg J, Divino CM, Chen S-H (2006) Gr-1+ CD115+ immature myeloid suppressor cells mediate the development of tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host. Cancer Res 66:1123–1131. https://doi.org/10.1158/0008-5472.CAN-05-1299 CrossRefPubMed

23.

Hanson EM, Clements VK, Sinha P, Ilkovitch D, Ostrand-Rosenberg S (2009) Myeloid-derived suppressor cells down-regulate L-selectin expression on CD4+ and CD8+ T cells. J Immunol 183:937–944. https://doi.org/10.4049/jimmunol.0804253 CrossRefPubMed

24.

Schmid M, Zimara N, Wege AK, Ritter U (2014) Myeloid-derived suppressor cell functionality and interaction with Leishmania major parasites differ in C57BL/6 and BALB/c mice. Eur J Immunol 44:3295–3306. https://doi.org/10.1002/eji.201344335 CrossRefPubMed

25.

Su N, Yue Y, Xiong S (2016) Monocytic myeloid-derived suppressor cells from females, but not males, alleviate CVB3-induced myocarditis by increasing regulatory and CD4(+)IL-10(+) T cells. Sci Rep 6:22658. https://doi.org/10.1038/srep22658 CrossRefPubMedPubMedCentral

26.

Carretero-Iglesia L, Bouchet-Delbos L, Louvet C, Drujont L, Segovia M, Merieau E, Chiffoleau E, Josien R, Hill M, Cuturi M-C, Moreau A (2016) Comparative study of the immunoregulatory capacity of in vitro generated tolerogenic dendritic cells, suppressor macrophages, and myeloid-derived suppressor cells. Transplantation 100:2079–2089. https://doi.org/10.1097/TP.0000000000001315 CrossRefPubMed

27.

Sierra RA, Thevenot P, Raber PL, Cui Y, Parsons C, Ochoa AC, Trillo-Tinoco J, Del Valle L, Rodriguez PC (2014) Rescue of notch-1 signaling in antigen-specific CD8+ T cells overcomes tumor-induced T-cell suppression and enhances immunotherapy in cancer. Cancer Immunol Res 2:800–811. https://doi.org/10.1158/2326-6066.CIR-14-0021 CrossRefPubMedPubMedCentral

28.

Corzo CA, Condamine T, Lu L, Cotter MJ, Youn J-I, Cheng P, Cho H-I, Celis E, Quiceno DG, Padhya T, McCaffrey TV, McCaffrey JC, Gabrilovich DI (2010) HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment. J Exp Med 207:2439–2453. https://doi.org/10.1084/jem.20100587 CrossRefPubMedPubMedCentral

29.

Bronte V, Wang M, Overwijk WW, Surman DR, Pericle F, Rosenberg SA, Restifo NP (1998) Apoptotic death of CD8+ T lymphocytes after immunization: induction of a suppressive population of Mac-1+/Gr-1+ cells. J Immunol 161:5313–5320PubMed

30.

Moses K, Klein JC, Männ L, Klingberg A, Gunzer M, Brandau S (2016) Survival of residual neutrophils and accelerated myelopoiesis limit the efficacy of antibody-mediated depletion of Ly-6G+ cells in tumor-bearing mice. J Leukoc Biol 99:811–823. https://doi.org/10.1189/jlb.1HI0715-289R CrossRefPubMed

31.

Clavijo PE, Moore EC, Chen J, Davis RJ, Friedman J, Kim Y, Van Waes C, Chen Z, Allen CT (2017) Resistance to CTLA-4 checkpoint inhibition reversed through selective elimination of granulocytic myeloid cells. Oncotarget 8:55804–55820. https://doi.org/10.18632/oncotarget.18437 CrossRefPubMedPubMedCentral

32.

Vincent J, Mignot G, Chalmin F, Ladoire S, Bruchard M, Chevriaux A, Martin F, Apetoh L, Rébé C, Ghiringhelli F (2010) 5-Fluorouracil selectively kills tumor-associated myeloid-derived suppressor cells resulting in enhanced T cell-dependent antitumor immunity. Cancer Res 70:3052–3061. https://doi.org/10.1158/0008-5472.CAN-09-3690 CrossRefPubMed

33.

Highfill SL, Cui Y, Giles AJ, Smith JP, Zhang H, Morse E, Kaplan RN, Mackall CL (2014) Disruption of CXCR2-mediated MDSC tumor trafficking enhances anti-PD1 efficacy. Sci Transl Med 6:237ra67. https://doi.org/10.1126/scitranslmed.3007974 CrossRefPubMedPubMedCentral

34.

Nefedova Y, Fishman M, Sherman S, Wang X, Beg AA, Gabrilovich DI (2007) Mechanism of all-trans retinoic acid effect on tumor-associated myeloid-derived suppressor cells. Cancer Res 67:11021–11028. https://doi.org/10.1158/0008-5472.CAN-07-2593 CrossRefPubMed

35.

Serafini P, Meckel K, Kelso M, Noonan K, Califano J, Koch W, Dolcetti L, Bronte V, Borrello I (2006) Phosphodiesterase-5 inhibition augments endogenous antitumor immunity by reducing myeloid-derived suppressor cell function. J Exp Med 203:2691–2702. https://doi.org/10.1084/jem.20061104 CrossRefPubMedPubMedCentral

36.

De Santo C, Serafini P, Marigo I, Dolcetti L, Bolla M, Del Soldato P, Melani C, Guiducci C, Colombo MP, Iezzi M, Musiani P, Zanovello P, Bronte V (2005) Nitroaspirin corrects immune dysfunction in tumor-bearing hosts and promotes tumor eradication by cancer vaccination. Proc Natl Acad Sci USA 102:4185–4190. https://doi.org/10.1073/pnas.0409783102 CrossRefPubMedPubMedCentral

37.

Nagaraj S, Youn J-I, Weber H, Iclozan C, Lu L, Cotter MJ, Meyer C, Becerra CR, Fishman M, Antonia S, Sporn MB, Liby KT, Rawal B, Lee J-H, Gabrilovich DI (2010) Anti-inflammatory triterpenoid blocks immune suppressive function of MDSCs and improves immune response in cancer. Clin Cancer Res 16:1812–1823. https://doi.org/10.1158/1078-0432.CCR-09-3272 CrossRefPubMedPubMedCentral

38.

Yu J, Du W, Yan F, Wang Y, Li H, Cao S, Yu W, Shen C, Liu J, Ren X (2013) Myeloid-derived suppressor cells suppress antitumor immune responses through IDO expression and correlate with lymph node metastasis in patients with breast cancer. J Immunol 190:3783–3797. https://doi.org/10.4049/jimmunol.1201449 CrossRefPubMed

39.

Hoechst B, Ormandy LA, Ballmaier M, Lehner F, Krüger C, Manns MP, Greten TF, Korangy F (2008) A new population of myeloid-derived suppressor cells in hepatocellular carcinoma patients induces CD4(+)CD25(+)Foxp3(+) T cells. Gastroenterology 135:234–243. https://doi.org/10.1053/j.gastro.2008.03.020 CrossRefPubMed

40.

Brandau S, Trellakis S, Bruderek K, Schmaltz D, Steller G, Elian M, Suttmann H, Schenck M, Welling J, Zabel P, Lang S (2011) Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties. J Leukoc Biol 89:311–317. https://doi.org/10.1189/jlb.0310162 CrossRefPubMed

41.

Obermajer N, Muthuswamy R, Lesnock J, Edwards RP, Kalinski P (2011) Positive feedback between PGE2 and COX2 redirects the differentiation of human dendritic cells toward stable myeloid-derived suppressor cells. Blood 118:5498–5505. https://doi.org/10.1182/blood-2011-07-365825 CrossRefPubMedPubMedCentral

42.

Pinton L, Solito S, Damuzzo V, Francescato S, Pozzuoli A, Berizzi A, Mocellin S, Rossi CR, Bronte V, Mandruzzato S (2016) Activated T cells sustain myeloid-derived suppressor cell-mediated immune suppression. Oncotarget 7:1168–1184. https://doi.org/10.18632/oncotarget.6662 CrossRefPubMed

43.

Jordan KR, Kapoor P, Spongberg E, Tobin RP, Gao D, Borges VF, McCarter MD (2017) Immunosuppressive myeloid-derived suppressor cells are increased in splenocytes from cancer patients. Cancer Immunol Immunother 66:503–513. https://doi.org/10.1007/s00262-016-1953-z CrossRefPubMedPubMedCentral

44.

Lechner MG, Liebertz DJ, Epstein AL (2010) Characterization of cytokine-induced myeloid-derived suppressor cells from normal human peripheral blood mononuclear cells. J Immunol 185:2273–2284. https://doi.org/10.4049/jimmunol.1000901 CrossRefPubMed

45.

Mandruzzato S, Brandau S, Britten CM, Bronte V, Damuzzo V, Gouttefangeas C, Maurer D, Ottensmeier C, van der Burg SH, Welters MJP, Walter S (2016) Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry: results from an interim study. Cancer Immunol Immunother 65:161–169. https://doi.org/10.1007/s00262-015-1782-5 CrossRefPubMedPubMedCentral

46.

Dumitru CA, Moses K, Trellakis S, Lang S, Brandau S (2012) Neutrophils and granulocytic myeloid-derived suppressor cells: immunophenotyping, cell biology and clinical relevance in human oncology. Cancer Immunol Immunother 61:1155–1167. https://doi.org/10.1007/s00262-012-1294-5 CrossRefPubMed

47.

Kusmartsev S, Nagaraj S, Gabrilovich DI (2005) Tumor-associated CD8+ T cell tolerance induced by bone marrow-derived immature myeloid cells. J Immunol 175:4583–4592CrossRefPubMed

48.

Heuvers ME, Muskens F, Bezemer K, Lambers M, Dingemans A-MC, Groen HJM, Smit EF, Hoogsteden HC, Hegmans JPJJ., Aerts JGJV. (2013) Arginase-1 mRNA expression correlates with myeloid-derived suppressor cell levels in peripheral blood of NSCLC patients. Lung Cancer 81:468–474. https://doi.org/10.1016/j.lungcan.2013.06.005 CrossRefPubMed

49.

Rodriguez PC, Hernandez CP, Quiceno D, Dubinett SM, Zabaleta J, Ochoa JB, Gilbert J, Ochoa AC (2005) Arginase I in myeloid suppressor cells is induced by COX-2 in lung carcinoma. J Exp Med 202:931–939. https://doi.org/10.1084/jem.20050715 CrossRefPubMedPubMedCentral

50.

Rodriguez PC, Ernstoff MS, Hernandez C, Atkins M, Zabaleta J, Sierra R, Ochoa AC (2009) Arginase I-producing myeloid-derived suppressor cells in renal cell carcinoma are a subpopulation of activated granulocytes. Cancer Res 69:1553–1560. https://doi.org/10.1158/0008-5472.CAN-08-1921 CrossRefPubMedPubMedCentral

51.

Liu C-Y, Wang Y-M, Wang C-L, Feng P-H, Ko H-W, Liu Y-H, Wu Y-C, Chu Y, Chung F-T, Kuo C-H, Lee K-Y, Lin S-M, Lin H-C, Wang C-H, Yu C-T, Kuo H-P (2010) Population alterations of l-arginase- and inducible nitric oxide synthase-expressed CD11b+/CD14−/CD15+/CD33+ myeloid-derived suppressor cells and CD8+ T lymphocytes in patients with advanced-stage non-small cell lung cancer. J Cancer Res Clin Oncol 136:35–45. https://doi.org/10.1007/s00432-009-0634-0 CrossRefPubMed

52.

Toor SM, Syed Khaja AS, El Salhat H, Bekdache O, Kanbar J, Jaloudi M, Elkord E (2016) Increased levels of circulating and tumor-infiltrating granulocytic myeloid cells in colorectal cancer patients. Front Immunol 7:560. https://doi.org/10.3389/fimmu.2016.00560 CrossRefPubMedPubMedCentral

53.

Munn DH, Shafizadeh E, Attwood JT, Bondarev I, Pashine A, Mellor AL (1999) Inhibition of T cell proliferation by macrophage tryptophan catabolism. J Exp Med 189:1363–1372CrossRefPubMedPubMedCentral

54.

Toor SM, Syed Khaja AS, El Salhat H, Faour I, Kanbar J, Quadri AA, Albashir M, Elkord E (2017) Myeloid cells in circulation and tumor microenvironment of breast cancer patients. Cancer Immunol Immunother 66:753–764. https://doi.org/10.1007/s00262-017-1977-z CrossRefPubMedPubMedCentral

55.

Cao LY, Chung J-S, Teshima T, Feigenbaum L, Cruz PD, Jacobe HT, Chong BF, Ariizumi K (2016) Myeloid-derived suppressor cells in psoriasis are an expanded population exhibiting diverse T-cell-suppressor mechanisms. J Invest Dermatol 136:1801–1810. https://doi.org/10.1016/j.jid.2016.02.816 CrossRefPubMedPubMedCentral

56.

Wesolowski R, Markowitz J, Carson WE (2013) Myeloid derived suppressor cells—a new therapeutic target in the treatment of cancer. J Immunother Cancer 1:10. https://doi.org/10.1186/2051-1426-1-10 CrossRefPubMedPubMedCentral

57.

Luyckx A, Schouppe E, Rutgeerts O, Lenaerts C, Fevery S, Devos T, Dierickx D, Waer M, Van Ginderachter JA, Billiau AD (2012) G-CSF stem cell mobilization in human donors induces polymorphonuclear and mononuclear myeloid-derived suppressor cells. Clin Immunol 143:83–87. https://doi.org/10.1016/j.clim.2012.01.011 CrossRefPubMed

58.

Walsh NC, Kenney LL, Jangalwe S, Aryee K-E, Greiner DL, Brehm MA, Shultz LD (2017) Humanized mouse models of clinical disease. Annu Rev Pathol 12:187–215. https://doi.org/10.1146/annurev-pathol-052016-100332 CrossRefPubMed

59.

Wu H, Zhen Y, Ma Z, Li H, Yu J, Xu Z-G, Wang X-Y, Yi H, Yang Y-G (2016) Arginase-1-dependent promotion of TH17 differentiation and disease progression by MDSCs in systemic lupus erythematosus. Sci Transl Med 8:331ra40. https://doi.org/10.1126/scitranslmed.aae0482 CrossRefPubMedPubMedCentral

60.

Liu G, Hu Y, Xiao J, Li X, Li Y, Tan H, Zhao Y, Cheng D, Shi H (2016) 99mTc-labelled anti-CD11b SPECT/CT imaging allows detection of plaque destabilization tightly linked to inflammation. Sci Rep 6:20900. https://doi.org/10.1038/srep20900 CrossRefPubMedPubMedCentral

61.

Eisenblaetter M, Flores-Borja F, Lee JJ, Wefers C, Smith H, Hueting R, Cooper MS, Blower PJ, Patel D, Rodriguez-Justo M, Milewicz H, Vogl T, Roth J, Tutt A, Schaeffter T, Ng T (2017) Visualization of tumor-immune interaction—target-specific imaging of S100A8/A9 reveals pre-metastatic niche establishment. Theranostics 7:2392–2401. https://doi.org/10.7150/thno.17138 CrossRefPubMedPubMedCentral

62.

Moses K, Brandau S (2016) Human neutrophils: their role in cancer and relation to myeloid-derived suppressor cells. Semin Immunol 28:187–196. https://doi.org/10.1016/j.smim.2016.03.018 CrossRefPubMed

63.

Condamine T, Dominguez GA, Youn J-I, Kossenkov AV, Mony S, Alicea-Torres K, Tcyganov E, Hashimoto A, Nefedova Y, Lin C, Partlova S, Garfall A, Vogl DT, Xu X, Knight SC, Malietzis G, Lee GH, Eruslanov E, Albelda SM et al (2016) Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients. Sci Immunol 1:aaf8943. https://doi.org/10.1126/sciimmunol.aaf8943 CrossRefPubMedPubMedCentral

64.

Trellakis S, Bruderek K, Hütte J, Elian M, Hoffmann TK, Lang S, Brandau S (2013) Granulocytic myeloid-derived suppressor cells are cryosensitive and their frequency does not correlate with serum concentrations of colony-stimulating factors in head and neck cancer. Innate Immun 19:328–336. https://doi.org/10.1177/1753425912463618 CrossRefPubMed

65.

Gregori S, Tomasoni D, Pacciani V, Scirpoli M, Battaglia M, Magnani CF, Hauben E, Roncarolo M-G (2010) Differentiation of type 1 T regulatory cells (Tr1) by tolerogenic DC-10 requires the IL-10-dependent ILT4/HLA-G pathway. Blood 116:935–944. https://doi.org/10.1182/blood-2009-07-234872 CrossRefPubMed

66.

Stiff A, Trikha P, Mundy-Bosse BL, McMichael EL, Mace TA, Benner B, Kendra K, Campbell A, Gautam S, Abood D, Landi I, Hsu V, Duggan MC, Wesolowski R, Old M, Howard JH, Yu L, Stasik N, Olencki T et al (2018) Nitric oxide production by myeloid derived suppressor cells plays a role in impairing Fc receptor-mediated natural killer cell function. Clin Cancer Res. https://doi.org/10.1158/1078-0432.CCR-17-0691 CrossRefPubMedPubMedCentral

67.

Mao Y, Sarhan D, Steven A, Seliger B, Kiessling R, Lundqvist A (2014) Inhibition of tumor-derived prostaglandin-e2 blocks the induction of myeloid-derived suppressor cells and recovers natural killer cell activity. Clin Cancer Res 20:4096–4106. https://doi.org/10.1158/1078-0432.CCR-14-0635 CrossRefPubMed

68.

Goh CC, Roggerson KM, Lee H-C, Golden-Mason L, Rosen HR, Hahn YS (2016) Hepatitis C virus-induced myeloid-derived suppressor cells suppress NK Cell IFN-γ production by altering cellular metabolism via arginase-1. J Immunol 196:2283–2292. https://doi.org/10.4049/jimmunol.1501881 CrossRefPubMed

Titel: How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions
verfasst von: Annika M. Bruger
Anca Dorhoi
Gunes Esendagli
Katarzyna Barczyk-Kahlert
Pierre van der Bruggen
Marie Lipoldova
Tomas Perecko
Juan Santibanez
Margarida Saraiva
Jo A. Van Ginderachter
Sven Brandau
Publikationsdatum: 21.05.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Cancer Immunology, Immunotherapy / Ausgabe 4/2019
Print ISSN: 0340-7004
Elektronische ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-018-2170-8

Neu im Fachgebiet Onkologie

24.04.2024 | DGIM 2024 | Nachrichten

Springer Medizin

How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions

Abstract

Neu im Fachgebiet Onkologie

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Krebspatienten impfen: Was? Wen? Und wann nicht?

Müde im Alter? Auch an die Nebenniere denken

Update Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2019

Role of myeloid regulatory cells (MRCs) in maintaining tissue homeostasis and promoting tolerance in autoimmunity, inflammatory disease and transplantation

Mutations resulting in the formation of hyperactive complement convertases support cytocidal effect of anti-CD20 immunotherapeutics

MDSC and beyond: a symposium-in-writing on myeloid cells with immunoregulatory activity by members of the Mye-EUNITER network

Deciphering myeloid-derived suppressor cells: isolation and markers in humans, mice and non-human primates

Adverse events need for hospitalization and systemic immunosuppression in very elderly patients (over 80 years) treated with ipilimumab for metastatic melanoma

Immune checkpoint inhibitor-induced colitis as a predictor of survival in metastatic melanoma

Neu im Fachgebiet Onkologie

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Krebspatienten impfen: Was? Wen? Und wann nicht?

Müde im Alter? Auch an die Nebenniere denken

Update Onkologie