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P-27/HP Endolysin as Antibacterial Agent for Antibiotic Resistant Staphylococcus aureus of Human Infections

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Abstract

Staphylococcus aureus causes a wide range of suppurative infections in humans and animals. Due to its high virulence, ability to adopt various environmental conditions, and acquired multiple drug resistance, treatment of such infections has become difficult. Therefore, there is an immense need to develop alternate drug modalities to control this pathogen. In past few years, phage-encoded endolysin therapy has emerged as a new hope not only due to its ability to specifically kill the target bacteria irrespective of their antibiotic sensitivity but also because of minimum or no side effects, a problem associated with antibiotic therapy. In this article, we report purification of a broad spectrum anti-staphylococcal endolysin (P-27/HP endolysin) encoded by phage P-27/HP isolated from sewage water. On SDS-PAGE endolysin resolved in three polypeptides of molecular weights 33.5, 48.6, and 62.2 kDa. Endolysin exhibited maximum in vitro lytic activity at temperature between 35 and 40°C and pH 7.0. In vivo experiments revealed considerable (99.9%) elimination of S. aureus 27/HP from spleens of endolysin-treated mice and had saved them from death due to bacteremia caused by S. aureus 27/HP challenge infection. Thus, P-27/HP endolysin offers suitable substitute of antibiotics to control S. aureus infections.

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Authors declare that they have no conflict of interest.

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  1. Ragini Gupta

    Present address: Department of Chemical Engineering, Indian Institute of Technology Kanpur, Kanpur, 208016, Uttar Pradesh, India

Authors and Affiliations

  1. Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India

    Ragini Gupta

  2. Department of Animal Science, M.J.P. Rohilkhand University, Bareilly, 243016, Uttar Pradesh, India

    Ragini Gupta & Yogendra Prasad

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  1. Ragini Gupta
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  2. Yogendra Prasad
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Correspondence to Ragini Gupta.

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Gupta, R., Prasad, Y. P-27/HP Endolysin as Antibacterial Agent for Antibiotic Resistant Staphylococcus aureus of Human Infections. Curr Microbiol 63, 39–45 (2011). https://doi.org/10.1007/s00284-011-9939-8

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  • DOI: https://doi.org/10.1007/s00284-011-9939-8

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