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01.07.2010 | Original Article

Effects of (−)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE₂, and IL-8 expression induced by IL-1β in human synovial fibroblasts

verfasst von: Guo-Shu Huang, Ching-Ya Tseng, Chian-Her Lee, Sui-Long Su, Herng-Sheng Lee

Erschienen in: Rheumatology International | Ausgabe 9/2010

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Abstract

The objective of this study was to examine the effects of (−)-epigallocatechin-3-gallate (EGCG) on cyclooxygenase 2 (COX-2), prostaglandin E₂ (PGE₂), and interleukin 8 (IL-8) expression induced by IL-1β in human synovial fibroblasts. Cells were enzymatically isolated from synovial tissue taken from patients undergoing joint replacement surgery for osteoarthritis. Reverse transcriptase-polymerase chain reaction, immunocytochemistry, and western blotting were used to assess the COX-2 gene and protein expression with the associated mechanisms. PGE₂ and IL-8 secretion into the culture medium was assayed by enzyme-linked immunosorbent assay. COX-2 upregulation in synovial fibroblasts induced by IL-1β was significantly suppressed by EGCG in a dose-dependent manner. PGE₂ and IL-8 secretion was also induced by IL-1β stimulation and significantly suppressed by EGCG. The mechanism was associated with the phosphorylation of IKKβ. EGCG may inhibit the expression of inflammatory mediators, such as COX-2, PGE₂, and IL-8, induced by IL-1β in human synovial fibroblasts. EGCG may be of value in the treatment of synovial inflammation.

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Titel: Effects of (−)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE2, and IL-8 expression induced by IL-1β in human synovial fibroblasts
verfasst von: Guo-Shu Huang
Ching-Ya Tseng
Chian-Her Lee
Sui-Long Su
Herng-Sheng Lee
Publikationsdatum: 01.07.2010
Verlag: Springer-Verlag
Erschienen in: Rheumatology International / Ausgabe 9/2010
Print ISSN: 0172-8172
Elektronische ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-009-1128-8

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Effects of (−)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE₂, and IL-8 expression induced by IL-1β in human synovial fibroblasts

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Leitlinien kompakt für die Innere Medizin

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Parodontalbehandlung verbessert Prognose bei Katheterablation

Prostatakarzinom: EU initiiert neues Screeningkonzept

Blasenkarzinom – Biomarker statt Zytologie?

Antihypertensiva schützen auch alte Menschen noch vor Demenz

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