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Erschienen in:
10.08.2016 | Computed Tomography
DNA damage in lymphocytes induced by cardiac CT and comparison with physical exposure parameters
Erschienen in: European Radiology | Ausgabe 4/2017
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Objectives
To investigate whether physical exposure parameters such as the dose index (CTDI), dose length product (DLP), and size-specific dose estimate (SSDE) are predictive of DNA damage.
Methods
In vitro, we scanned a phantom containing blood samples from five volunteers at CTDI 50, 100, and 150 mGy. One sample was not scanned. We also scanned samples in three different-size phantoms at CTDI 100 mGy. In vivo, we enrolled 45 patients and obtained blood samples before and after cardiac CT. The γ-H2AX foci were counted.
Results
In vitro, in the control and at CTDI 50, 100, and 150 mGy, the number of γ-H2AX was 0.94 ± 0.24 (standard error, SE), 1.28 ± 0.30, 1.91 ± 0.47, and 2.16 ± 0.20. At SSDE 180, 156, and 135 mGy, it was 2.41 ± 0.20, 1.91 ± 0.47, and 1.42 ± 0.20 foci/cell. The γ-H2AX foci were positively correlated with the radiation dose and negatively correlated with the body size. In vivo, the γ-H2AX foci were significantly increased after CT (from 1.21 ± 0.19 to 1.92 ± 0.22 foci/cell) and correlated with CTDI, DLP, and SSDE.
Conclusions
DNA damage was induced by cardiac CT. There was a correlation between the physical exposure parameters and γ-H2AX.
Key Points
• DNA damage was induced by radiation exposure from cardiac CT.
• The γ-H2AX foci number was correlated with the CT radiation dose.
• Physical exposure parameters reflect the DNA damage by CT radiation exposure.