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Erschienen in: World Journal of Urology 2/2015

01.02.2015 | Original Article

Urinary bladder mucosal responses to ischemia

verfasst von: Masataka Sunagawa, Amanda Wolf-Johnston, Masanori Nomiya, Norifumi Sawada, Karl-Erik Andersson, Tadashi Hisamitsu, Lori A. Birder

Erschienen in: World Journal of Urology | Ausgabe 2/2015

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Abstract

Purpose

The objectives of this study were to examine the expression of various cellular proteins within the urothelium (UT) and lamina propria (LP) following chronic bladder ischemia in the rat urinary bladder.

Materials and methods

Urinary bladders were removed from adult Sprague–Dawley rats 8 weeks after creation of bladder ischemia and from sham controls. Immunocytochemistry was used to examine distribution of LP-vimentin-immunoreactive (IR) cells and connexins (Cx26; Cx43), and western immunoblotting or ELISA for proteins involved in UT barrier and sensory functions.

Results

Ischemia was associated with a significant increase in LP-vimentin-IR cells and increased expression of the gap junction proteins Cx26 and Cx43 within the bladder UT as compared to sham control. Ischemia also resulted in an increased (p < 0.05) expression level of the junctional marker (ZO-1) and non-significantly increased expressions of the trophic factor nerve growth factor as well as norepinephrine.

Conclusions

Our findings reveal that chronic ischemia alters a number of proteins within the UT and underlying LP. These proteins are involved in barrier function, remodeling, repair as well as intercellular communication. The increased expression of LP-vimentin-IR cells suggests that changes in cell–cell interactions could play a role in ischemia-induced changes in bladder activity.
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Metadaten
Titel
Urinary bladder mucosal responses to ischemia
verfasst von
Masataka Sunagawa
Amanda Wolf-Johnston
Masanori Nomiya
Norifumi Sawada
Karl-Erik Andersson
Tadashi Hisamitsu
Lori A. Birder
Publikationsdatum
01.02.2015
Verlag
Springer Berlin Heidelberg
Erschienen in
World Journal of Urology / Ausgabe 2/2015
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI
https://doi.org/10.1007/s00345-014-1298-1

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