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Pediatric Surgery International

Erschienen in:

01.12.2008 | Original Article

Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells

verfasst von: Marc Reismann, Daniel Lehmann, Matthias Quandte, Benno M. Ure, Sylvia Glüer

Erschienen in: Pediatric Surgery International | Ausgabe 12/2008

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Abstract

Purpose

The aim of the current study was to investigate effects of CO₂ atmosphere, mimicking conditions of the pneumoperitoneum during laparoscopy, on epigenetic conditions of Rassf1A and DCR2 oncogenes in neuroblastoma cells.

Methods

SH-SY5Y neuroblastoma cells were exposed to 100% CO₂ for 4 h. Cells were lysed 4, 8 and 168 h after exposure. After methylation analysis of Rassf1A and DCR2 with polymerase chain reaction, results were compared to those of physiologically incubated neuroblastoma cells.

Results

No significant changes were found after exposure to carbon dioxide compared to the control. Values of methylated Rassf1A were 12.6 ± 1.1 versus 13.2 ± 1.4 ng/μl in the controls, respectively (4 h after incubation), 12.6 ± 1.2 versus 15.1 ± 0.9 ng/μl (8 h) and 14.2 ± 1.5 versus 11.7 ± 1.3 ng/μl (168 h). DCR2 showed values of 4.6 ± 0.5 versus 3.7 ± 0.5 ng/μl (4 h), 3.8 ± 0.5 versus 4.1 ± 0.4 ng/μl (8 h) and 3.6 ± 0.4 versus 3.8 ± 0.5 ng/μl (168 h).

Conclusion

Exposure of neuroblastoma cells to 100% CO₂ does not alter methylation of two prognostic relevant index genes. It seems therefore unlikely that effects on methylation levels within CO₂ pneumoperitoneum lead to epigenetic changes in neuroblastoma.

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Titel: Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells
verfasst von: Marc Reismann
Daniel Lehmann
Matthias Quandte
Benno M. Ure
Sylvia Glüer
Publikationsdatum: 01.12.2008
Verlag: Springer-Verlag
Erschienen in: Pediatric Surgery International / Ausgabe 12/2008
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-008-2274-x

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Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells

Abstract

Purpose

Methods

Results

Conclusion

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