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Erschienen in: Basic Research in Cardiology 1/2016

01.01.2016 | Original Contribution

Characterization of apela, a novel endogenous ligand of apelin receptor, in the adult heart

verfasst von: Ábel Perjés, Teemu Kilpiö, Johanna Ulvila, Johanna Magga, Tarja Alakoski, Zoltán Szabó, Laura Vainio, Eveliina Halmetoja, Olli Vuolteenaho, Ulla Petäjä-Repo, István Szokodi, Risto Kerkelä

Erschienen in: Basic Research in Cardiology | Ausgabe 1/2016

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Abstract

The G protein-coupled apelin receptor regulates important processes of the cardiovascular homeostasis, including cardiac development, cardiac contractility, and vascular tone. Most recently, a novel endogenous peptide ligand for the apelin receptor was identified in zebrafish, and it was named apela/elabela/toddler. The peptide was originally considered as an exclusively embryonic regulator, and so far its function in the adult organism remains elusive. We show here that apela is predominantly expressed in the non-cardiomyocyte fraction in the adult rodent heart. We also provide evidence that apela binds to apelin receptors in the heart. Using isolated adult rat hearts, we demonstrate, that just like the fellow receptor agonist apelin, apela increases cardiac contractility and induces coronary vasodilation already in the nanomolar level. The inotropic effect, as revealed by Western blot analysis, is accompanied by a significant increase in extracellular signal-regulated kinase (ERK) 1/2 phosphorylation. Pharmacological inhibition of ERK1/2 activation markedly attenuates the apela-induced inotropy. Analysis of samples from infarcted mouse hearts showed that expression of both apela and apelin receptor is induced in failing mouse hearts and correlate with left ventricular ejection fraction. Hence, we conclude that apela is present in the adult heart, is upregulated in post-infarction cardiac remodeling, and increases cardiac contractility in an ERK1/2-dependent manner.
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Metadaten
Titel
Characterization of apela, a novel endogenous ligand of apelin receptor, in the adult heart
verfasst von
Ábel Perjés
Teemu Kilpiö
Johanna Ulvila
Johanna Magga
Tarja Alakoski
Zoltán Szabó
Laura Vainio
Eveliina Halmetoja
Olli Vuolteenaho
Ulla Petäjä-Repo
István Szokodi
Risto Kerkelä
Publikationsdatum
01.01.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
Basic Research in Cardiology / Ausgabe 1/2016
Print ISSN: 0300-8428
Elektronische ISSN: 1435-1803
DOI
https://doi.org/10.1007/s00395-015-0521-6

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