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Erschienen in: Archives of Orthopaedic and Trauma Surgery 8/2008

01.08.2008 | Basic Science

Biological effects of extracorporeal shockwave in bone healing: a study in rabbits

verfasst von: Ching-Jen Wang, Feng-Sheng Wang, Kuender D. Yang

Erschienen in: Archives of Orthopaedic and Trauma Surgery | Ausgabe 8/2008

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Abstract

Introduction

This study is an investigation of the biological effects of extracorporeal shockwave treatment (ESWT) on bone healing in a rabbit model.

Materials and methods

Sixteen 12-month-old New Zealand white rabbits with body weight ranging from 2.5 to 3.5 kg were used in the study. An intra-medullary pin was inserted retrograde into the femur canal. A closed fracture of the femur was created with a three-point bend method. The animals were randomly divided into the study group and the control group with eight rabbits in each group. The study group received shockwave treatment, whereas the control group did not. The animals were killed at 12 weeks, and a 5-cm long femur bone including the callus was harvested. The specimens were subjected to biomechanical study, histomorphological examination, and immunohistochemical analysis.

Results

The shockwave group showed significantly better bone strength in biomechanical study, more cortical bone formation in histomorphological examination and higher number of neo-vessels and angiogenic and osteogenic growth markers including VEGF, eNOS, PCNA, and BMP-2 on immunohistochemical stains than the control group.

Conclusion

ESWT significantly improved bone healing after fracture of the femur in rabbit. ESWT promoted the formation of cortical bone what might have been associated with increased biomechanical results. ESWT-promoted bone healing was associated with increased neovascularization and up-regulation of angiogenic and osteogenic growth factors.
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Metadaten
Titel
Biological effects of extracorporeal shockwave in bone healing: a study in rabbits
verfasst von
Ching-Jen Wang
Feng-Sheng Wang
Kuender D. Yang
Publikationsdatum
01.08.2008
Verlag
Springer-Verlag
Erschienen in
Archives of Orthopaedic and Trauma Surgery / Ausgabe 8/2008
Print ISSN: 0936-8051
Elektronische ISSN: 1434-3916
DOI
https://doi.org/10.1007/s00402-008-0663-1

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