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Investigation of c-myc and p53 Gene Alterations in the Tumor and Surgical Borderline Tissues of NSCLC and Effects on Clinicopathologic Behavior: By the FISH Technique

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Abstract

Genetic alterations on the primary tumoral tissues and surgical borderline tissues of 51 patients with NSCLC on which radiotherapy and chemotherapy had not been performed were analyzed by using the FISH method with locus-specific probes for p53 tumor suppressor gene and c-myc oncogene and centromere-specific probes for chromosome 17 and chromosome 8 on which these genes are located. P53 deletions were detected in 7 patients (13.7%), c-myc amplification in 4 patients (7.8%), monosomy 17 in 2 patients (3.9%) and trisomy 8 in 3 patients (5.8%), and a high level of polyploidy in tumoral tissues of 6 patients (11.7%). P53 deletion and c-myc amplification were found at surgical borderlines of 2 patients and 1 patient, respectively. Although both p53 deletion and c-myc amplification have low frequency at surgical border tissues, not only their detection is important for the follow-up of recurrency and metastasis, but it is also important for genetical and pathological staging. The results of this study show that c-myc amplification in NSCLC is related to the shortening of survival (p < 0.01). C-myc amplification and p53 deletion are also effective for the occurrence of metastasis (p < 0.05) and the effect of c-myc amplification in this matter is much higher than p53 deletion. The gain or loss of copy number of chromosome 8 and monosomy 17 show parallel effects with c-myc amplification and p53 deletion, respectively, on the clinicopathological behavior of tumors.

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Acknowledgements

We thank Drs. Bulent Ediz and Ilker Ercan for helping with statistical analysis, Dr. Nur Urer for pathological support, and Medical Biolog Barboros Yigit for his technical assistance. This study was supported by Research Foundation of Uludag University (Project number: 2001/39).

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Authors and Affiliations

  1. Departments of Medical Biology and Genetics, University of Uludag, Bursa, Turkey

    T. Yakut & U. Egeli

  2. Department of Thoracic Surgery, Faculty of Medicine, University of Uludag, Bursa, Turkey

    C. Gebitekin

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  1. T. Yakut
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Correspondence to T. Yakut.

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Yakut, T., Egeli, U. & Gebitekin, C. Investigation of c-myc and p53 Gene Alterations in the Tumor and Surgical Borderline Tissues of NSCLC and Effects on Clinicopathologic Behavior: By the FISH Technique . Lung 181, 245–258 (2003). https://doi.org/10.1007/s00408-003-1026-x

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