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International Journal of Legal Medicine

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01.09.2006 | Short Communication

Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

verfasst von: C. Robino, A. Giolitti, S. Gino, C. Torre

Erschienen in: International Journal of Legal Medicine | Ausgabe 5/2006

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Abstract

Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101–DXS7424 and DXS6789–DXS6801–DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.

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Titel: Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample
verfasst von: C. Robino
A. Giolitti
S. Gino
C. Torre
Publikationsdatum: 01.09.2006
Verlag: Springer-Verlag
Erschienen in: International Journal of Legal Medicine / Ausgabe 5/2006
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-006-0115-9

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