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Erschienen in: International Journal of Legal Medicine 3/2017

01.12.2016 | Original Article

Developmental validation of the HomyGene19+14Y System

verfasst von: Weian Du, Ling Chen, Hong Liu, Pingming Qiu, Fayuan Li, Jing Gao, Yu Zhou, Bangchao Wang, Chao Liu

Erschienen in: International Journal of Legal Medicine | Ausgabe 3/2017

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Abstract

The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications. In this study, the multiplex system was validated for sensitivity, specificity, DNA mixtures, stability, precision, stutter, reproducibility, parallel tests, PCR-based conditions, and population analysis according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation guidelines. A total of 212 alleles were detected for the 18 autosomal STR loci among 528 Guangdong Han individuals, and 431 haplotypes were found for 14 Y-STRs among 452 unrelated males. The combined match probability (CMP) of the HG19+14Y System was calculated as 2.39 × 10−29. All the validation results showed that the HG19+14Y System would be a robust, reliable, highly polymorphic, and informative forensic kit.
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Metadaten
Titel
Developmental validation of the HomyGene19+14Y System
verfasst von
Weian Du
Ling Chen
Hong Liu
Pingming Qiu
Fayuan Li
Jing Gao
Yu Zhou
Bangchao Wang
Chao Liu
Publikationsdatum
01.12.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
International Journal of Legal Medicine / Ausgabe 3/2017
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-016-1505-2

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