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Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology 10/2006

01.10.2006 | Laboratory Investigation

Effects of direct intravitreal dopamine injections on the development of lid-suture induced myopia in rabbits

verfasst von: Qianying Gao, Quan Liu, Ping Ma, XingWu Zhong, Junshu Wu, Jian Ge

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 10/2006

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Abstract

Background

Dopamine (DA) storage and release are reduced in form deprivation myopia (FDM) in a wide range of species, from chicks to primates. FDM can be prevented by treatment with DA agonists such as apomorphine, and paradoxically by the dopamine neurotoxin 6-hydroxydopamine. In this study, we increased the DA levels by direct intravitreal DA injections to learn if FDM can be suppressed in a rabbit model.

Methods

Seven-day-old rabbits were deprived of pattern vision by the suturing the right eyelids after natural eye opening. In the first group (FD, n=20), the right eye received form deprivation (FD) alone. In the second group (DA-FD, n=16), the deprived eye of 7-day-old rabbits received four intravitreal injections of 20 μg dopamine every 5 days. In the third group (saline-FD, n=16), the deprived eye received saline injections with the same schedule. The contralateral eye remained untreated as a control. At the end of the 8-week deprivation period, the effects of DA on refractive error, corneal curvature and ocular dimensions were assessed by streak retinoscopy, keratometry and A-scan ultrasonography, respectively.

Results

Eight weeks of FDM induced a myopic shift of −2.70±0.87 D (n=20) in treated eyes compared with contralateral eyes. The major structural correlate of the myopia appeared to be elongation of the vitreous chamber (0.7±0.3 mm, n=20) and axial elongation (0.9±0.3 mm, n=20), respectively. Repeated intravitreal injections of DA fully prevented the myopic shift (−0.06±0.37 D), elongation of the vitreous chamber (0.1±0.3 mm, n=16) and axial elongation (0.3±0.2 mm, n=16) due to lid suture, whereas saline injections had slight effect.

Conclusions

FD by suturing eyelids is an effective technique to induce a significant myopic shift, vitreous chamber and axial elongation in rabbits as a model of myopia development. These changes associated with FD were retarded by intravitreal injections of DA.
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Metadaten
Titel
Effects of direct intravitreal dopamine injections on the development of lid-suture induced myopia in rabbits
verfasst von
Qianying Gao
Quan Liu
Ping Ma
XingWu Zhong
Junshu Wu
Jian Ge
Publikationsdatum
01.10.2006
Verlag
Springer-Verlag
Erschienen in
Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 10/2006
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-006-0254-1

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