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Graefe's Archive for Clinical and Experimental Ophthalmology

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01.05.2012 | Medical Ophthalmology

A potential therapeutic strategy for inhibition of ocular neovascularization with a new endogenous protein: rhEDI-8t

verfasst von: Ling Zhang, Xi Shen, Qing Lu, Qingwei Zhou, Jiaqi Gu, Renbao Gan, Hui Zhang, Xiaodong Sun, Bing Xie

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 5/2012

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Abstract

Background

Endogenous angiogenesis inhibitors act as natural negative feedback in the focal area during the neovascularization process, and have less interference on physiological angiogenesis, and thus fewer negative side-effects. These inhibitors are potential candidates to combine with or substitutes for current popular anti-angiogenesis treatments to have synergistic effect. In this study, the effects of recombinant endothelial growth inhibitor protein (rhEDI-8t), a novel endogenous protein originated from collagen VIII, was investigated on ocular neovascularization (NV). Endostatin, a well-identified endogenous angiogenesis inhibitor, was compared in parallel and served as a positive control.

Methods

The inhibitory effect of rhEDI-8t on vascular endothelial cells was evaluated by a human umbilical vascular endothelial cells (HUVEC) proliferation test and a bovine aortic endothelial cells (BAEC) migration experiment. The effect of rhEDI-8t on ocular NV was further investigated in mice with choroidal neovascularization (choroidal NV) induced by laser, ischemic retinopathy and transgenic mice with expression of VEGF in photoreceptors (rho/VEGF) respectively.

Results

RhEDI-8t inhibited the growth of HUVECs and migration of BAECs stimulated by basic fibroblast growth factor (bFGF). Mice intravitreally treated with rhEDI-8t showed a significant reduction of choroidal NV, retinal NV and subretinal NV.

Conclusion

Endogenous angiogenesis inhibitor rhEDI-8t showed a potent anti-angiogenesis effect in both in vitro and in vivo experiments. It contributed to the suppression of ocular NV. The study suggested that rhEDI-8t could be a subsidiary potent therapeutic medicine in addition to anti-VEGF therapy in future clinical anti-angiogenesis treatment.

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Titel: A potential therapeutic strategy for inhibition of ocular neovascularization with a new endogenous protein: rhEDI-8t
verfasst von: Ling Zhang
Xi Shen
Qing Lu
Qingwei Zhou
Jiaqi Gu
Renbao Gan
Hui Zhang
Xiaodong Sun
Bing Xie
Publikationsdatum: 01.05.2012
Verlag: Springer-Verlag
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 5/2012
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-011-1765-y

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A potential therapeutic strategy for inhibition of ocular neovascularization with a new endogenous protein: rhEDI-8t

Abstract

Background

Methods

Results

Conclusion

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Ophthalmika in der Schwangerschaft

Operative Therapie und Keimnachweis bei endogener Endophthalmitis

Bakterielle endogene Endophthalmitis

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Update Augenheilkunde

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Background

Methods

Results

Conclusion

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