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Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology 2/2012

01.02.2012 | Glaucoma

Repeatability of nerve fiber layer thickness measurements in patients with glaucoma and without glaucoma using spectral-domain and time-domain OCT

verfasst von: Marc Töteberg-Harms, Veit Sturm, Pascal B. Knecht, Jens Funk, Marcel N. Menke

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 2/2012

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Abstract

Background

The aim of this work is to assess the repeatability of spectral-domain-OCT (SD-OCT) retinal nerve fiber layer thickness (RNFL) thickness measurements in a non-glaucoma group and patients with glaucoma and to compare these results to conventional time-domain-OCT (TD-OCT).

Methods

In a prospective, comparative, observational case-control study, 50 eyes of 25 non-glaucoma and 22 eyes of 11 patients with primary open angle glaucoma (POAG) were included. SD-OCT and TD-OCT circle scans were centered on the optic disc. In each eye, OCT scans were performed three times by two independent observers. RNFL thickness was measured in four quadrants around the optic disc. In addition, the overall mean RNFL thickness was assessed. Intraclass correlation coefficients (ICC) and coefficients of variation (COV) were calculated. Inter-observer and inter-OCT repeatability was visualized by using Bland–Altman analysis.

Results

Intra-observer repeatability for TD- OCT was good with an ICCmean RNFL thickness of 0.939 in non-glaucomas and 0.980 in glaucomatous eyes. For SD-OCT, intra-observer repeatability was higher with an ICC of 0.989 for non-glaucomas and 0.997 for glaucomatous eyes. COVs for TD-OCT ranged from 2.9–7.7% in non-glaucomas and from 6.0–13.3% in glaucoma patients. COVs for SD-OCT ranged from 0.3–1% in non-glaucomas and from 0.9–2.3% in glaucomatous eyes. COVs were influenced by various factors. In the glaucoma group, COVs were significantly higher (p < 0.001) compared to the non-glaucoma group. COVs increased by a mean of 5.1% when TD-OCT was used instead of SD-OCT (p < 0.001).

Conclusions

SD-OCT RNFL thickness measurements in healthy volunteers and glaucoma patients showed good intra- and inter-observer repeatability. Especially in glaucomatous eyes, repeatability of SD-OCT was superior to TD-OCT.
Literatur
1.
Zurück zum Zitat Chen TC, Zeng A, Sun W, Mujat M, de Boer JF (2008) Spectral domain optical coherence tomography and glaucoma. Int Ophthalmol Clin 48:29–45PubMedCrossRef Chen TC, Zeng A, Sun W, Mujat M, de Boer JF (2008) Spectral domain optical coherence tomography and glaucoma. Int Ophthalmol Clin 48:29–45PubMedCrossRef
2.
Zurück zum Zitat Cho JW, Sung KR, Hong JT, Um TW, Kang SY, Kook MS (2011)Detection of glaucoma by spectral domain-scanning laser ophthalmoscopy/optical coherence tomography (SD-SLO/OCT) and time domain optical coherence tomography. J Glaucoma 20:15–20PubMedCrossRef Cho JW, Sung KR, Hong JT, Um TW, Kang SY, Kook MS (2011)Detection of glaucoma by spectral domain-scanning laser ophthalmoscopy/optical coherence tomography (SD-SLO/OCT) and time domain optical coherence tomography. J Glaucoma 20:15–20PubMedCrossRef
3.
Zurück zum Zitat Leung CK, Cheung CY, Weinreb RN, Qiu K, Liu S, Li H, Xu G, Fan N, Pang CP, Tse KK, Lam DS (2010) Evaluation of retinal nerve fiber layer progression in glaucoma: a study on optical coherence tomography guided progression analysis. Invest Ophthalmol Vis Sci 51:217–222PubMedCrossRef Leung CK, Cheung CY, Weinreb RN, Qiu K, Liu S, Li H, Xu G, Fan N, Pang CP, Tse KK, Lam DS (2010) Evaluation of retinal nerve fiber layer progression in glaucoma: a study on optical coherence tomography guided progression analysis. Invest Ophthalmol Vis Sci 51:217–222PubMedCrossRef
4.
Zurück zum Zitat Park SB, Sung KR, Kang SY, Kim KR, Kook MS (2009) Comparison of glaucoma diagnostic capabilities of cirrus HD and stratus optical coherence tomography. Arch Ophthalmol 127:1603–1609PubMedCrossRef Park SB, Sung KR, Kang SY, Kim KR, Kook MS (2009) Comparison of glaucoma diagnostic capabilities of cirrus HD and stratus optical coherence tomography. Arch Ophthalmol 127:1603–1609PubMedCrossRef
5.
Zurück zum Zitat Zangwill LM, Bowd C, Weinreb RN (2000) Evaluating the optic disc and retinal nerve fiber layer in glaucoma. II: optical image analysis. Semin Ophthalmol 15:206–220PubMedCrossRef Zangwill LM, Bowd C, Weinreb RN (2000) Evaluating the optic disc and retinal nerve fiber layer in glaucoma. II: optical image analysis. Semin Ophthalmol 15:206–220PubMedCrossRef
6.
Zurück zum Zitat Schuman JS, Hee MR, Arya AV, Pedut-Kloizman T, Puliafito CA, Fujimoto JG, Swanson EA (1995) Optical coherence tomography: a new tool for glaucoma diagnosis. Curr Opin Ophthalmol 6:89–95PubMedCrossRef Schuman JS, Hee MR, Arya AV, Pedut-Kloizman T, Puliafito CA, Fujimoto JG, Swanson EA (1995) Optical coherence tomography: a new tool for glaucoma diagnosis. Curr Opin Ophthalmol 6:89–95PubMedCrossRef
7.
Zurück zum Zitat Blumenthal EZ, Williams JM, Weinreb RN, Girkin CA, Berry CC, Zangwill LM (2000) Reproducibility of nerve fiber layer thickness measurements by use of optical coherence tomography. Ophthalmology 107:2278–2282PubMedCrossRef Blumenthal EZ, Williams JM, Weinreb RN, Girkin CA, Berry CC, Zangwill LM (2000) Reproducibility of nerve fiber layer thickness measurements by use of optical coherence tomography. Ophthalmology 107:2278–2282PubMedCrossRef
8.
Zurück zum Zitat Bourne RR, Medeiros FA, Bowd C, Jahanbakhsh K, Zangwill LM, Weinreb RN (2005) Comparability of retinal nerve fiber layer thickness measurements of optical coherence tomography instruments. Invest Ophthalmol Vis Sci 46:1280–1285PubMedCrossRef Bourne RR, Medeiros FA, Bowd C, Jahanbakhsh K, Zangwill LM, Weinreb RN (2005) Comparability of retinal nerve fiber layer thickness measurements of optical coherence tomography instruments. Invest Ophthalmol Vis Sci 46:1280–1285PubMedCrossRef
9.
Zurück zum Zitat Budenz DL, Chang RT, Huang X, Knighton RW, Tielsch JM (2005) Reproducibility of retinal nerve fiber thickness measurements using the stratus OCT in normal and glaucomatous eyes. Invest Ophthalmol Vis Sci 46:2440–2443PubMedCrossRef Budenz DL, Chang RT, Huang X, Knighton RW, Tielsch JM (2005) Reproducibility of retinal nerve fiber thickness measurements using the stratus OCT in normal and glaucomatous eyes. Invest Ophthalmol Vis Sci 46:2440–2443PubMedCrossRef
10.
Zurück zum Zitat Budenz DL, Fredette MJ, Feuer WJ, Anderson DR (2008) Reproducibility of peripapillary retinal nerve fiber thickness measurements with stratus OCT in glaucomatous eyes. Ophthalmology 115(661–666):e664 Budenz DL, Fredette MJ, Feuer WJ, Anderson DR (2008) Reproducibility of peripapillary retinal nerve fiber thickness measurements with stratus OCT in glaucomatous eyes. Ophthalmology 115(661–666):e664
11.
Zurück zum Zitat Carpineto P, Ciancaglini M, Zuppardi E, Falconio G, Doronzo E, Mastropasqua L (2003) Reliability of nerve fiber layer thickness measurements using optical coherence tomography in normal and glaucomatous eyes. Ophthalmology 110:190–195PubMedCrossRef Carpineto P, Ciancaglini M, Zuppardi E, Falconio G, Doronzo E, Mastropasqua L (2003) Reliability of nerve fiber layer thickness measurements using optical coherence tomography in normal and glaucomatous eyes. Ophthalmology 110:190–195PubMedCrossRef
12.
Zurück zum Zitat Krist R, Hoffmann EM, Schwenn O (2005) Reproducibility of measurements of the peripapillary retinal nerve fibre layer thickness. Optical coherence tomography versus retinal thickness analyzer. Ophthalmologe 102(1175–1178):1180 Krist R, Hoffmann EM, Schwenn O (2005) Reproducibility of measurements of the peripapillary retinal nerve fibre layer thickness. Optical coherence tomography versus retinal thickness analyzer. Ophthalmologe 102(1175–1178):1180
13.
Zurück zum Zitat Paunescu LA, Schuman JS, Price LL, Stark PC, Beaton S, Ishikawa H, Wollstein G, Fujimoto JG (2004) Reproducibility of nerve fiber thickness, macular thickness, and optic nerve head measurements using StratusOCT. Invest Ophthalmol Vis Sci 45:1716–1724PubMedCrossRef Paunescu LA, Schuman JS, Price LL, Stark PC, Beaton S, Ishikawa H, Wollstein G, Fujimoto JG (2004) Reproducibility of nerve fiber thickness, macular thickness, and optic nerve head measurements using StratusOCT. Invest Ophthalmol Vis Sci 45:1716–1724PubMedCrossRef
14.
Zurück zum Zitat Choma M, Sarunic M, Yang C, Izatt J (2003) Sensitivity advantage of swept source and Fourier domain optical coherence tomography. Opt Express 11:2183–2189PubMedCrossRef Choma M, Sarunic M, Yang C, Izatt J (2003) Sensitivity advantage of swept source and Fourier domain optical coherence tomography. Opt Express 11:2183–2189PubMedCrossRef
15.
Zurück zum Zitat Wojtkowski M, Srinivasan V, Fujimoto JG, Ko T, Schuman JS, Kowalczyk A, Duker JS (2005) Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography. Ophthalmology 112:1734–1746PubMedCrossRef Wojtkowski M, Srinivasan V, Fujimoto JG, Ko T, Schuman JS, Kowalczyk A, Duker JS (2005) Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography. Ophthalmology 112:1734–1746PubMedCrossRef
16.
Zurück zum Zitat Wojtkowski M, Srinivasan V, Ko T, et al (2005) High-speed, ultrahigh resolution retinal imaging using spectral/Fourier domain OCTConf Lasers Electrooptics, pp. 2058–2060 Wojtkowski M, Srinivasan V, Ko T, et al (2005) High-speed, ultrahigh resolution retinal imaging using spectral/Fourier domain OCTConf Lasers Electrooptics, pp. 2058–2060
17.
Zurück zum Zitat Wojtkowski M, Srinivasan V, Ko T, Fujimoto J, Kowalczyk A, Duker J (2004) Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation. Opt Express 12:2404–2422PubMedCrossRef Wojtkowski M, Srinivasan V, Ko T, Fujimoto J, Kowalczyk A, Duker J (2004) Ultrahigh-resolution, high-speed, Fourier domain optical coherence tomography and methods for dispersion compensation. Opt Express 12:2404–2422PubMedCrossRef
18.
Zurück zum Zitat Bendschneider D, Tornow RP, Horn FK, Laemmer R, Roessler CW, Juenemann AG, Kruse FE, Mardin CY (2010) Retinal nerve fiber layer thickness in normals measured by spectral domain OCT. J Glaucoma 19:475–482PubMedCrossRef Bendschneider D, Tornow RP, Horn FK, Laemmer R, Roessler CW, Juenemann AG, Kruse FE, Mardin CY (2010) Retinal nerve fiber layer thickness in normals measured by spectral domain OCT. J Glaucoma 19:475–482PubMedCrossRef
19.
Zurück zum Zitat Gabriele ML, Ishikawa H, Wollstein G, Bilonick RA, Kagemann L, Wojtkowski M, Srinivasan VJ, Fujimoto JG, Duker JS, Schuman JS (2007) Peripapillary nerve fiber layer thickness profile determined with high speed, ultrahigh resolution optical coherence tomography high-density scanning. Invest Ophthalmol Vis Sci 48:3154–3160PubMedCrossRef Gabriele ML, Ishikawa H, Wollstein G, Bilonick RA, Kagemann L, Wojtkowski M, Srinivasan VJ, Fujimoto JG, Duker JS, Schuman JS (2007) Peripapillary nerve fiber layer thickness profile determined with high speed, ultrahigh resolution optical coherence tomography high-density scanning. Invest Ophthalmol Vis Sci 48:3154–3160PubMedCrossRef
20.
Zurück zum Zitat Hong S, Kim CY, Lee WS, Seong GJ (2010) Reproducibility of peripapillary retinal nerve fiber layer thickness with spectral domain cirrus high-definition optical coherence tomography in normal eyes. Jpn J Ophthalmol 54:43–47PubMedCrossRef Hong S, Kim CY, Lee WS, Seong GJ (2010) Reproducibility of peripapillary retinal nerve fiber layer thickness with spectral domain cirrus high-definition optical coherence tomography in normal eyes. Jpn J Ophthalmol 54:43–47PubMedCrossRef
21.
Zurück zum Zitat Menke MN, Knecht P, Sturm V, Dabov S, Funk J (2008) Reproducibility of nerve fiber layer thickness measurements using 3D Fourier-domain OCT. Invest Ophthalmol Vis Sci 49:5386–5391PubMedCrossRef Menke MN, Knecht P, Sturm V, Dabov S, Funk J (2008) Reproducibility of nerve fiber layer thickness measurements using 3D Fourier-domain OCT. Invest Ophthalmol Vis Sci 49:5386–5391PubMedCrossRef
22.
Zurück zum Zitat Rabe-Hesketh S, Skrondal A (2008) Estimation using xtmixed in Multilevel and Longitudinal Modeling Using STATA, pp. 433–436 Rabe-Hesketh S, Skrondal A (2008) Estimation using xtmixed in Multilevel and Longitudinal Modeling Using STATA, pp. 433–436
23.
Zurück zum Zitat Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1:307–310PubMedCrossRef Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1:307–310PubMedCrossRef
24.
Zurück zum Zitat Bland JM, Altman DG (1999) Measuring agreement in method comparison studies. Stat Methods Med Res 8:135–160PubMedCrossRef Bland JM, Altman DG (1999) Measuring agreement in method comparison studies. Stat Methods Med Res 8:135–160PubMedCrossRef
25.
Zurück zum Zitat Gonzalez-Garcia AO, Vizzeri G, Bowd C, Medeiros FA, Zangwill LM, Weinreb RN (2009) Reproducibility of RTVue retinal nerve fiber layer thickness and optic disc measurements and agreement with Stratus optical coherence tomography measurements. Am J Ophthalmol 147:1067–1074, 1074 e1061PubMedCrossRef Gonzalez-Garcia AO, Vizzeri G, Bowd C, Medeiros FA, Zangwill LM, Weinreb RN (2009) Reproducibility of RTVue retinal nerve fiber layer thickness and optic disc measurements and agreement with Stratus optical coherence tomography measurements. Am J Ophthalmol 147:1067–1074, 1074 e1061PubMedCrossRef
26.
Zurück zum Zitat Johnson DE, El-Defrawy SR, Almeida DR, Campbell RJ (2009) Comparison of retinal nerve fibre layer measurements from time domain and spectral domain optical coherence tomography systems. Can J Ophthalmol 44:562–566PubMedCrossRef Johnson DE, El-Defrawy SR, Almeida DR, Campbell RJ (2009) Comparison of retinal nerve fibre layer measurements from time domain and spectral domain optical coherence tomography systems. Can J Ophthalmol 44:562–566PubMedCrossRef
27.
Zurück zum Zitat Kim JS, Ishikawa H, Sung KR, Xu J, Wollstein G, Bilonick RA, Gabriele ML, Kagemann L, Duker JS, Fujimoto JG, Schuman JS (2009) Retinal nerve fibre layer thickness measurement reproducibility improved with spectral domain optical coherence tomography. Br J Ophthalmol 93:1057–1063PubMedCrossRef Kim JS, Ishikawa H, Sung KR, Xu J, Wollstein G, Bilonick RA, Gabriele ML, Kagemann L, Duker JS, Fujimoto JG, Schuman JS (2009) Retinal nerve fibre layer thickness measurement reproducibility improved with spectral domain optical coherence tomography. Br J Ophthalmol 93:1057–1063PubMedCrossRef
28.
Zurück zum Zitat Knight OJ, Chang RT, Feuer WJ, Budenz DL (2009) Comparison of retinal nerve fiber layer measurements using time domain and spectral domain optical coherent tomography. Ophthalmology 116:1271–1277PubMedCrossRef Knight OJ, Chang RT, Feuer WJ, Budenz DL (2009) Comparison of retinal nerve fiber layer measurements using time domain and spectral domain optical coherent tomography. Ophthalmology 116:1271–1277PubMedCrossRef
29.
Zurück zum Zitat Sung KR, Kim DY, Park SB, Kook MS (2009) Comparison of retinal nerve fiber layer thickness measured by Cirrus HD and Stratus optical coherence tomography. Ophthalmology 116:1264–1270, 1270 e1261PubMedCrossRef Sung KR, Kim DY, Park SB, Kook MS (2009) Comparison of retinal nerve fiber layer thickness measured by Cirrus HD and Stratus optical coherence tomography. Ophthalmology 116:1264–1270, 1270 e1261PubMedCrossRef
30.
Zurück zum Zitat Vizzeri G, Weinreb RN, Gonzalez-Garcia AO, Bowd C, Medeiros FA, Sample PA, Zangwill LM (2009) Agreement between spectral-domain and time-domain OCT for measuring RNFL thickness. Br J Ophthalmol 93:775–781PubMedCrossRef Vizzeri G, Weinreb RN, Gonzalez-Garcia AO, Bowd C, Medeiros FA, Sample PA, Zangwill LM (2009) Agreement between spectral-domain and time-domain OCT for measuring RNFL thickness. Br J Ophthalmol 93:775–781PubMedCrossRef
31.
Zurück zum Zitat Leite MT, Zangwill LM, Weinreb RN, Rao HL, Alencar LM, Sample PA, Medeiros FA (2010) Effect of disease severity on the performance of Cirrus spectral-domain OCT for glaucoma diagnosis. Invest Ophthalmol Vis Sci 51:4104–4109PubMedCrossRef Leite MT, Zangwill LM, Weinreb RN, Rao HL, Alencar LM, Sample PA, Medeiros FA (2010) Effect of disease severity on the performance of Cirrus spectral-domain OCT for glaucoma diagnosis. Invest Ophthalmol Vis Sci 51:4104–4109PubMedCrossRef
32.
Zurück zum Zitat Jones AL, Sheen NJ, North RV, Morgan JE (2001) The Humphrey optical coherence tomography scanner: quantitative analysis and reproducibility study of the normal human retinal nerve fibre layer. Br J Ophthalmol 85:673–677PubMedCrossRef Jones AL, Sheen NJ, North RV, Morgan JE (2001) The Humphrey optical coherence tomography scanner: quantitative analysis and reproducibility study of the normal human retinal nerve fibre layer. Br J Ophthalmol 85:673–677PubMedCrossRef
33.
Zurück zum Zitat Schuman JS, Pedut-Kloizman T, Hertzmark E, Hee MR, Wilkins JR, Coker JG, Puliafito CA, Fujimoto JG, Swanson EA (1996) Reproducibility of nerve fiber layer thickness measurements using optical coherence tomography. Ophthalmology 103:1889–1898PubMed Schuman JS, Pedut-Kloizman T, Hertzmark E, Hee MR, Wilkins JR, Coker JG, Puliafito CA, Fujimoto JG, Swanson EA (1996) Reproducibility of nerve fiber layer thickness measurements using optical coherence tomography. Ophthalmology 103:1889–1898PubMed
34.
Zurück zum Zitat Chang RT, Knight OJ, Feuer WJ, Budenz DL (2009) Sensitivity and specificity of time-domain versus spectral-domain optical coherence tomography in diagnosing early to moderate glaucoma. Ophthalmology 116:2294–2299PubMedCrossRef Chang RT, Knight OJ, Feuer WJ, Budenz DL (2009) Sensitivity and specificity of time-domain versus spectral-domain optical coherence tomography in diagnosing early to moderate glaucoma. Ophthalmology 116:2294–2299PubMedCrossRef
35.
Zurück zum Zitat Kim JS, Ishikawa H, Gabriele ML, Wollstein G, Bilonick RA, Kagemann L, Fujimoto JG, Schuman JS (2010) Retinal nerve fiber layer thickness measurement comparability between time domain optical coherence tomography (OCT) and spectral domain OCT. Invest Ophthalmol Vis Sci 51:896–902PubMedCrossRef Kim JS, Ishikawa H, Gabriele ML, Wollstein G, Bilonick RA, Kagemann L, Fujimoto JG, Schuman JS (2010) Retinal nerve fiber layer thickness measurement comparability between time domain optical coherence tomography (OCT) and spectral domain OCT. Invest Ophthalmol Vis Sci 51:896–902PubMedCrossRef
36.
Zurück zum Zitat Leung CK, Cheung CY, Weinreb RN, Qiu Q, Liu S, Li H, Xu G, Fan N, Huang L, Pang CP, Lam DS (2009) Retinal nerve fiber layer imaging with spectral-domain optical coherence tomography: a variability and diagnostic performance study. Ophthalmology 116:1257–1263, 1263 e1251–1252PubMedCrossRef Leung CK, Cheung CY, Weinreb RN, Qiu Q, Liu S, Li H, Xu G, Fan N, Huang L, Pang CP, Lam DS (2009) Retinal nerve fiber layer imaging with spectral-domain optical coherence tomography: a variability and diagnostic performance study. Ophthalmology 116:1257–1263, 1263 e1251–1252PubMedCrossRef
37.
Zurück zum Zitat Schuman JS (2008) Spectral domain optical coherence tomography for glaucoma (an AOS thesis). Trans Am Ophthalmol Soc 106:426–458PubMed Schuman JS (2008) Spectral domain optical coherence tomography for glaucoma (an AOS thesis). Trans Am Ophthalmol Soc 106:426–458PubMed
38.
Zurück zum Zitat Knighton RW, Qian C (2000) An optical model of the human retinal nerve fiber layer: implications of directional reflectance for variability of clinical measurements. J Glaucoma 9:56–62PubMedCrossRef Knighton RW, Qian C (2000) An optical model of the human retinal nerve fiber layer: implications of directional reflectance for variability of clinical measurements. J Glaucoma 9:56–62PubMedCrossRef
Metadaten
Titel
Repeatability of nerve fiber layer thickness measurements in patients with glaucoma and without glaucoma using spectral-domain and time-domain OCT
verfasst von
Marc Töteberg-Harms
Veit Sturm
Pascal B. Knecht
Jens Funk
Marcel N. Menke
Publikationsdatum
01.02.2012
Verlag
Springer-Verlag
Erschienen in
Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 2/2012
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-011-1811-9

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