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Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes

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Abstract

Adipose tissue contains a stroma that can be easily isolated. Thus, human adipose tissue presents an source of multipotent stromal cells. In order to determine the implication of hematopoietic markers in adipocyte biology, we have defined part of the phenotype of the human adipose tissue-derived stromal cells, and compared this to fully differentiated adipocytes. Flow cytometry demonstrates that the protein expression phenotype of both cell types are similar and includes the expression of CD10, CD13, CD34, CD36, CD55, CD59 and CD65. No significant difference between subcutaneous and omental adipose tissue could be demonstrated concerning the expression of these markers. However, the expression of CD34, CD36 and CD65 is cell-dependent. While the expression of CD36 and CD65 doubled between stromal cells and mature adipocytes, the expression of CD34 decreased, despite this protein being present on the mature adipocyte. As CD34 is described as a stem cell marker and it being unlikely to be expressed on differentiated cells, this result was confirmed by immunostaining and western blot. The clear function of this protein on the adipocyte membrane remains to be determined. The characterization of new proteins on mature adipocytes could have broad implications for the comprehension of the biology of this tissue.

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Authors and Affiliations

  1. Laboratoire de Biochimie et Génétique Moléculaire, EA-2526, Université de La Réunion, Faculté des Sciences, 15 Avenue René Cassin, 97415, Saint Denis, France

    Franck Festy, Laurence Hoareau, Sandrine Bes-Houtmann, Marie-Paule Gonthier, Maya Césari & Régis Roche

  2. Service de Biologie, Centre Hospitalier Départemental Félix Guyon, Saint Denis, Ile de La Réunion, France

    Anne-Marie Péquin

  3. Service de Chirurgie Digestive, Centre Hospitalier Départemental Félix Guyon, Saint Denis, Ile de La Réunion, France

    Ashik Munstun

  4. Brain Inflammation and Immunity Group, University of Wales College of Medicine, Cardiff, UK

    Jean Jacques Hoarau

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  1. Franck Festy
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  2. Laurence Hoareau
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  5. Marie-Paule Gonthier
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  7. Jean Jacques Hoarau
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  8. Maya Césari
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  9. Régis Roche
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Correspondence to Régis Roche.

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Festy, F., Hoareau, L., Bes-Houtmann, S. et al. Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes. Histochem Cell Biol 124, 113–121 (2005). https://doi.org/10.1007/s00418-005-0014-z

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  • DOI: https://doi.org/10.1007/s00418-005-0014-z

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