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European Journal of Applied Physiology

Erschienen in:

01.12.2007 | Original Article

Effect of a single session of electrical stimulation on activity and expression of citrate synthase and antioxidant enzymes in rat soleus muscle

verfasst von: Aurélio da Silva Pimenta, Rafael Herling Lambertucci, Renata Gorjão, Leonardo dos Reis Silveira, Rui Curi

Erschienen in: European Journal of Applied Physiology | Ausgabe 1/2007

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Abstract

The aim of our study was to investigate the effect of a single high intensity session of muscle contractions on the activity and expression of citrate synthase (CS) and of the following major antioxidant enzymes: Mn-superoxide dismutase (Mn-SOD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), and glutathione peroxidase (GPX). To accomplish this, soleus muscles of male Wistar rats were subjected to contractions using a intense electrical stimulation (ES) protocol. Soleus muscles were isolated either immediately or 1 h after the contractions and utilized for enzyme activity determination, and for analysis of gene expression by quantitative PCR. A significant increase in maximal activity (63%) and expression (80%) of CS was observed in stimulated soleus muscles, isolated 1 h after ES as compared to controls. However, this effect was not observed in muscles isolated immediately after ES. By using macroarray and Real Time RT-PCR analysis, an increase in expression of Mn-SOD, Cu,Zn-SOD, CAT, and GPX was also found. Interestingly, of these enzymes, only CAT activity was significantly increased (44%) 1 h after ES in soleus muscle. These results indicate that acute ES up-regulates activity and expression of CS and CAT in soleus muscles. This increase in expression of CAT may play an important role in counteracting the potential deleterious effects of elevated oxidative stress induced by a high oxidative demand in skeletal muscles subjected to exercise training.

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Titel: Effect of a single session of electrical stimulation on activity and expression of citrate synthase and antioxidant enzymes in rat soleus muscle
verfasst von: Aurélio da Silva Pimenta
Rafael Herling Lambertucci
Renata Gorjão
Leonardo dos Reis Silveira
Rui Curi
Publikationsdatum: 01.12.2007
Verlag: Springer-Verlag
Erschienen in: European Journal of Applied Physiology / Ausgabe 1/2007
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-007-0542-4

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Abstract

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