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Oxygen sensing and conducted vasomotor responses in mouse cremaster arterioles in situ

  • Cardiovascular Physiology
  • Published:
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Abstract

This study examines mechanisms by which changes in tissue oxygen tension elicit vasomotor responses and whether localized changes in oxygen tension initiates conducted vasomotor responses in mouse cremaster arterioles. Intravital microscopy was used to visualize the mouse cremaster microcirculation. The cremaster was superfused with Krebs’ solution with different oxygen tensions, and a gas exchange chamber was used to induce localized changes in oxygen tension. In arterioles where red blood cells were removed by buffer perfusion, arterioles responded with same magnitudes of vasodilatation (ΔD = 16.0 ± 4.9 μm) when changing from high (PO2 = 242.5 ± 13.3 mm Hg) to low (PO2 = 22.5 ± 4.8 mm Hg) oxygen tension as seen in the intact cremaster circulation (ΔD = 18.7 ± 1.0 μm). Blockade of NO synthases by L-NAME and adenosine receptors by DPCPX had no effects on vasomotor responses to low or high oxygen. Induction of localized low (PO2 = 23.3 ± 5.7 mmHg) or high (PO2 = 300.0 ± 25.7 mm Hg) oxygen tension caused vasodilatation or -constriction locally and at a site 1,000 μm upstream (distantly). Glibenclamide blocker of ATP-sensitive K+ channels inhibited vasodilatation and -constriction to low (PO2 = 16.0 ± 6.4 mm Hg) and high (PO2 = 337.4 ± 12.8 mm Hg) oxygen tension. 1) ATP-sensitive K+ channels seem to mediate, at least in part, vasodilatation and vasoconstriction to low and high oxygen tension; 2) Red blood cells are not necessary for inducing vasodilatation and vasoconstriction to low or high oxygen tension; 3) localized changes in the oxygen tension cause vasomotor responses, which are conducted upstream along arterioles in mouse cremaster microcirculation.

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Acknowledgments

This study was supported by grants from the Danish Medical Research Council for Health and Disease, the Novo-Nordisk Foundation, the Lundbeck Foundation, and the Danish Heart Association.

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Authors and Affiliations

  1. Division of Renal & Vascular Research, Department of Biomedical Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen, Denmark

    Anh Thuc Ngo, Lars Jørn Jensen, Mads Riemann & Niels-Henrik Holstein-Rathlou

  2. Department of Cardiology, Gentofte Hospital, Copenhagen, Denmark

    Christian Torp-Pedersen

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  1. Anh Thuc Ngo
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Correspondence to Anh Thuc Ngo.

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Ngo, A.T., Jensen, L.J., Riemann, M. et al. Oxygen sensing and conducted vasomotor responses in mouse cremaster arterioles in situ. Pflugers Arch - Eur J Physiol 460, 41–53 (2010). https://doi.org/10.1007/s00424-010-0837-x

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  • DOI: https://doi.org/10.1007/s00424-010-0837-x

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