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Green tea extract given before regional myocardial ischemia–reperfusion in rats improves myocardial contractility by attenuating calcium overload

  • Cardiovascular Physiology
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

There is evidence for a negative correlation between green tea consumption and cardiovascular diseases. The aim of the present study was to examine whether green tea extract (GTE) given before regional myocardial ischemia could improve depression of myocardial contractility by preventing cytosolic Ca2+ overload. Regional ischemia–reperfusion (IR) was induced in rats by ligating the left anterior descending branch for 20 min, then releasing the ligature. Ligation induced ventricular arrhythmias in rats without GTE pretreatment, but decreased arrhythmogenesis was seen in rats pretreated 30 min earlier with GTE (400 mg/kg). During reperfusion, arrhythmias only occurred during the initial 5 min, and GTE pretreatment had no effect. After overnight recovery, serum cTnI levels were greatly increased in control post-IR rats but only slightly elevated in GTE-pretreated post-IR rats. Myocardial contractility measured by echocardiography was still depressed after 3 days in control post-IR rats, but not in GTE-pretreated post-IR rats. No myocardial ischemic injury was seen in post-IR rats with or without GTE pretreatment. Using freshly isolated single heart myocytes, GTE was found to attenuate the post-IR injury-associated cytosolic Ca2+ overload and modulate changes in the levels and distribution of myofibril, adherens junction, and gap junction proteins. In summary, GTE pretreatment protects cardiomyocytes from IR injury by preventing cytosolic Ca2+ overload, myofibril disruption, and alterations in adherens and gap junction protein expression and distribution.

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Acknowledgments

This work was supported by the National Science Council of Taiwan (grant NSC 98-2320-B-005-005 to Y-M L and grants NSC 98-2314-B-010-033-MY2 and 98-2314-B-075A-011-MY2 to T.J. W). The authors wish to express special thanks to Mrs. C.H. Chiu, Ms. D.C. Tsai, Mr. P.T. Tseng, and Mr. T.C. Chen for their technical assistance and animal care.

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Authors and Affiliations

  1. Department of Life Sciences, National Chung-Hsing University, 250 Kuokang Road, Taichung, 402, Taiwan

    Ying-Ming Liou & Jan-Yow Chen

  2. Department of Cardiovascular Surgery, Taichung Veterans General Hospital, Taichung, 407, Taiwan

    Shih-Rong Hsieh

  3. Cardiovascular Center, Taichung Veterans General Hospital, Taichung, 407, Taiwan

    Tsu-Juey Wu

  4. National Yang-Ming University School of Medicine, Taipei, Taiwan

    Tsu-Juey Wu

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  1. Ying-Ming Liou
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Correspondence to Ying-Ming Liou.

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Liou, YM., Hsieh, SR., Wu, TJ. et al. Green tea extract given before regional myocardial ischemia–reperfusion in rats improves myocardial contractility by attenuating calcium overload. Pflugers Arch - Eur J Physiol 460, 1003–1014 (2010). https://doi.org/10.1007/s00424-010-0881-6

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