Introduction
Materials and methods
Mouse mutants
Tissue preparation and immunofluorescence
Antigen | Host | Dilution | Supplier | Catalog no |
---|---|---|---|---|
HA | Mouse | 1:1000 | Covance | MMS-101R |
GFP | Chicken | 1:1000 | Life Technologies | A10262 |
CR | Rabbit | 1:1000 | Swant | 7699/3H |
CB | Rabbit | 1:1000 | Swant | CB382 |
PV | Rabbit | 1:1000 | Swant | PV25 |
mGluR1α | Rabbit | 1:500 | Abnova | PAB14526 |
NPY | Rabbit | 1:500 | Abcam | ab10980 |
SOM | Rabbit | 1:300 | Millipore | AB5494 |
nNOS | Mouse | 1:300 | Sigma | N2280 |
RLN | Mouse | 1:500 | Millipore | MAB5364 |
VGLUT3 | Guinea pig | 1:500 | Gift from El Mestikawy | |
CB1R | Rabbit | 1:1000 | Frontier Institute | CB1-Rb-Af380 |
D2R | Rabbit | 1:500 | Frontier Institute | D2R-Rb-Af960 |
Figures | GFP/markers | s.o. | s.p. | s.r. | s.l-m |
---|---|---|---|---|---|
Figure 1b | GFP | 411 | 288 | 421 | 145 |
PV | 271 | 400 | 37 | 5 | |
GFP/PV | 153 | 89 | 9 | 0 | |
Figure 1d | GFP | 372 | 272 | 421 | 139 |
CB | 217 | ND | 177 | 35 | |
GFP/CB | 82 | 55 | 84 | 18 | |
Figure 1f | GFP | 428 | 286 | 476 | 173 |
CR | 76 | 144 | 154 | 261 | |
GFP/CR | 16 | 31 | 25 | 3 | |
Figure 2b | GFP | 469 | 294 | 564 | 208 |
NPY | 297 | 171 | 223 | 104 | |
GFP/NPY | 188 | 111 | 174 | 58 | |
Figure 2d | GFP | 401 | 216 | 396 | 138 |
SOM | 351 | 21 | 17 | 0 | |
GFP/SOM | 136 | 12 | 9 | 0 | |
Figure 3b | GFP | 214 | 141 | 244 | 94 |
nNOS | 141 | 123 | 206 | 71 | |
GFP/nNOS | 110 | 88 | 139 | 44 | |
Figure 3d | GFP | 212 | 123 | 137 | 65 |
RLN | 229 | 33 | 235 | 359 | |
GFP/RLN | 120 | 15 | 87 | 29 | |
Figure 4b | GFP | 353 | 229 | 371 | 68 |
mGluR1α | 271 | 43 | 95 | 0 | |
GFP/mGluR1α | 149 | 15 | 52 | 0 | |
Figure 5d | GFP | 220 | 108 | 216 | 110 |
D2R | 236 | 143 | 247 | 34 | |
GFP/D2R | 181 | 69 | 146 | 15 |
Polyribosome immunoprecipitation
cDNA synthesis and quantitative real-time PCR
Marker | PCR primers |
---|---|
Gfap
|
Sense, AGCGAGCGTGCAGAGATGA |
Antisense, AGGAAGCGGACCTTCTCGAT | |
Cnp1
|
Sense, GCTGCACTGTACAACCAAATTCTG |
Antisense, ACCTCCTGCTGGGCGTATT | |
Iba1
|
Sense, CCCCCAGCCAAGAAAGCTAT |
Antisense, GCCCCACCGTGTGACATC | |
Camk2a
|
Sense, TTTGAGGAACTGGGAAAGGG |
Antisense, CATGGAGTCGGACGATATTGG | |
Slc1a1
|
Sense, AAAGATAGCAGGAAGGTAACCGAAT |
Antisense, CGGTCAGTCGGTAGCTTTCAG | |
Calb2
|
Sense, TGAGAATGAACTGGACGCCCTC |
Antisense, GTAGAGCTTCCCTGCCTCGG | |
Gad1
|
Sense, TTGTGCTTTGCTGTGTTTTAGAGA |
Antisense, CCCCCTGCCCAAAGATAGAC | |
Sst
|
Sense, CTGTCCTGCCGTCTCCAGTG |
Antisense, CTCTGTCTGGTTGGGCTCGG | |
Slc32a1
|
Sense, TCACGACAAACCCAAGATCAC |
Antisense, GTCTTCGTTCTCCTCGTACAG | |
Drd2
|
Sense, CTCTTTGGACTCAACAACACAGA |
Antisense, AAGGGCACGTAGAACGAGAC | |
Drd1a
|
Sense, TCGAACTGTATGGTGCCCTT |
Antisense, TGGGGTTCAGGGAGGAATTC |