Skip to main content
Erschienen in: Medical Microbiology and Immunology 1/2011

01.02.2011 | Rapid Communication

Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells

verfasst von: Junji Xing, Shuai Wang, You Li, Hong Guo, Lei Zhao, Weiwei Pan, Fusen Lin, Huifang Zhu, Lei Wang, Meili Li, Lin Wang, Chunfu Zheng

Erschienen in: Medical Microbiology and Immunology | Ausgabe 1/2011

Einloggen, um Zugang zu erhalten

Abstract

In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.
Literatur
2.
Zurück zum Zitat Simpson JC, Neubrand VE, Wiemann S, Pepperkok R (2001) Illuminating the human genome. Histochem Cell Biol 115(1):23–29PubMed Simpson JC, Neubrand VE, Wiemann S, Pepperkok R (2001) Illuminating the human genome. Histochem Cell Biol 115(1):23–29PubMed
3.
Zurück zum Zitat Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y (2003) Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol 77(2):1382–1391CrossRefPubMed Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y (2003) Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol 77(2):1382–1391CrossRefPubMed
8.
Zurück zum Zitat Xing J, Wu F, Pan W, Zheng C (2010) Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells. Virus Res 153(1):71–81CrossRefPubMed Xing J, Wu F, Pan W, Zheng C (2010) Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells. Virus Res 153(1):71–81CrossRefPubMed
10.
Zurück zum Zitat Calder JM, Stow EC, Stow ND (1992) On the cellular localization of the components of the herpes simplex virus type 1 helicase-primase complex and the viral origin-binding protein. J Gen Virol 73(Pt 3):531–538CrossRefPubMed Calder JM, Stow EC, Stow ND (1992) On the cellular localization of the components of the herpes simplex virus type 1 helicase-primase complex and the viral origin-binding protein. J Gen Virol 73(Pt 3):531–538CrossRefPubMed
13.
Zurück zum Zitat Chen Y, Ciustea M, Ricciardi RP (2005) Processivity factor of KSHV contains a nuclear localization signal and binding domains for transporting viral DNA polymerase into the nucleus. Virology 340(2):183–191CrossRefPubMed Chen Y, Ciustea M, Ricciardi RP (2005) Processivity factor of KSHV contains a nuclear localization signal and binding domains for transporting viral DNA polymerase into the nucleus. Virology 340(2):183–191CrossRefPubMed
14.
Zurück zum Zitat Carter KL, Ward PL, Roizman B (1996) Characterization of the products of the U(L)43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription. J Virol 70(11):7663–7668PubMed Carter KL, Ward PL, Roizman B (1996) Characterization of the products of the U(L)43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription. J Virol 70(11):7663–7668PubMed
15.
Zurück zum Zitat Samaniego LA, Neiderhiser L, DeLuca NA (1998) Persistence and expression of the herpes simplex virus genome in the absence of immediate-early proteins. J Virol 72(4):3307–3320PubMed Samaniego LA, Neiderhiser L, DeLuca NA (1998) Persistence and expression of the herpes simplex virus genome in the absence of immediate-early proteins. J Virol 72(4):3307–3320PubMed
16.
Zurück zum Zitat Markovitz NS, Roizman B (2000) Small dense nuclear bodies are the site of localization of herpes simplex virus 1 U(L)3 and U(L)4 proteins and of ICP22 only when the latter protein is present. J Virol 74(1):523–528CrossRefPubMed Markovitz NS, Roizman B (2000) Small dense nuclear bodies are the site of localization of herpes simplex virus 1 U(L)3 and U(L)4 proteins and of ICP22 only when the latter protein is present. J Virol 74(1):523–528CrossRefPubMed
17.
Zurück zum Zitat Reynolds AE, Ryckman BJ, Baines JD, Zhou Y, Liang L, Roller RJ (2001) U(L)31 and U(L)34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids. J Virol 75(18):8803–8817CrossRefPubMed Reynolds AE, Ryckman BJ, Baines JD, Zhou Y, Liang L, Roller RJ (2001) U(L)31 and U(L)34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids. J Virol 75(18):8803–8817CrossRefPubMed
19.
Zurück zum Zitat Roizman B (1999) Hsv gene functions: what have we learned that could be generally applicable to its near and distant cousins? Acta Virol 43(2–3):75–80PubMed Roizman B (1999) Hsv gene functions: what have we learned that could be generally applicable to its near and distant cousins? Acta Virol 43(2–3):75–80PubMed
20.
Zurück zum Zitat Everett RD, Sourvinos G, Orr A (2003) Recruitment of herpes simplex virus type 1 transcriptional regulatory protein ICP4 into foci juxtaposed to ND10 in live, infected cells. J Virol 77(6):3680–3689CrossRefPubMed Everett RD, Sourvinos G, Orr A (2003) Recruitment of herpes simplex virus type 1 transcriptional regulatory protein ICP4 into foci juxtaposed to ND10 in live, infected cells. J Virol 77(6):3680–3689CrossRefPubMed
21.
Zurück zum Zitat Harland J, Dunn P, Cameron E, Conner J, Brown SM (2003) The herpes simplex virus (HSV) protein ICP34.5 is a virion component that forms a DNA-binding complex with proliferating cell nuclear antigen and HSV replication proteins. J Neurovirol 9(4):477–488. doi:AXPGX9NE3C7F07HM[pii] PubMed Harland J, Dunn P, Cameron E, Conner J, Brown SM (2003) The herpes simplex virus (HSV) protein ICP34.5 is a virion component that forms a DNA-binding complex with proliferating cell nuclear antigen and HSV replication proteins. J Neurovirol 9(4):477–488. doi:AXPGX9NE3C7F07HM​[pii] PubMed
22.
Zurück zum Zitat Hutchinson L, Browne H, Wargent V, Davis-Poynter N, Primorac S, Goldsmith K, Minson AC, Johnson DC (1992) A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH. J Virol 66(4):2240–2250PubMed Hutchinson L, Browne H, Wargent V, Davis-Poynter N, Primorac S, Goldsmith K, Minson AC, Johnson DC (1992) A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH. J Virol 66(4):2240–2250PubMed
24.
Zurück zum Zitat Yamada H, Jiang YM, Oshima S, Wada K, Goshima F, Daikoku T, Nishiyama Y (1998) Characterization of the UL4 gene product of herpes simplex virus type 2. Arch Virol 143(6):1199–1207CrossRefPubMed Yamada H, Jiang YM, Oshima S, Wada K, Goshima F, Daikoku T, Nishiyama Y (1998) Characterization of the UL4 gene product of herpes simplex virus type 2. Arch Virol 143(6):1199–1207CrossRefPubMed
25.
Zurück zum Zitat White CA, Stow ND, Patel AH, Hughes M, Preston VG (2003) Herpes simplex virus type 1 portal protein UL6 interacts with the putative terminase subunits UL15 and UL28. J Virol 77(11):6351–6358CrossRefPubMed White CA, Stow ND, Patel AH, Hughes M, Preston VG (2003) Herpes simplex virus type 1 portal protein UL6 interacts with the putative terminase subunits UL15 and UL28. J Virol 77(11):6351–6358CrossRefPubMed
28.
Zurück zum Zitat Malik AK, Martinez R, Muncy L, Carmichael EP, Weller SK (1992) Genetic analysis of the herpes simplex virus type 1 UL9 gene: isolation of a LacZ insertion mutant and expression in eukaryotic cells. Virology 190(2):702–715CrossRefPubMed Malik AK, Martinez R, Muncy L, Carmichael EP, Weller SK (1992) Genetic analysis of the herpes simplex virus type 1 UL9 gene: isolation of a LacZ insertion mutant and expression in eukaryotic cells. Virology 190(2):702–715CrossRefPubMed
33.
Zurück zum Zitat Yamauchi Y, Kiriyama K, Kubota N, Kimura H, Usukura J, Nishiyama Y (2008) The UL14 tegument protein of herpes simplex virus type 1 is required for efficient nuclear transport of the alpha transinducing factor VP16 and viral capsids. J Virol 82(3):1094–1106. doi:JVI.01226-07[pii]10.1128/JVI.01226-07 CrossRefPubMed Yamauchi Y, Kiriyama K, Kubota N, Kimura H, Usukura J, Nishiyama Y (2008) The UL14 tegument protein of herpes simplex virus type 1 is required for efficient nuclear transport of the alpha transinducing factor VP16 and viral capsids. J Virol 82(3):1094–1106. doi:JVI.​01226-07[pii]10.​1128/​JVI.​01226-07 CrossRefPubMed
36.
Zurück zum Zitat Rixon FJ, Addison C, McGregor A, Macnab SJ, Nicholson P, Preston VG, Tatman JD (1996) Multiple interactions control the intracellular localization of the herpes simplex virus type 1 capsid proteins. J Gen Virol 77(Pt 9):2251–2260CrossRefPubMed Rixon FJ, Addison C, McGregor A, Macnab SJ, Nicholson P, Preston VG, Tatman JD (1996) Multiple interactions control the intracellular localization of the herpes simplex virus type 1 capsid proteins. J Gen Virol 77(Pt 9):2251–2260CrossRefPubMed
38.
Zurück zum Zitat Takakuwa H, Goshima F, Koshizuka T, Murata T, Daikoku T, Nishiyama Y (2001) Herpes simplex virus encodes a virion-associated protein which promotes long cellular processes in over-expressing cells. Genes Cells 6(11):955–966. doi:475[pii] CrossRefPubMed Takakuwa H, Goshima F, Koshizuka T, Murata T, Daikoku T, Nishiyama Y (2001) Herpes simplex virus encodes a virion-associated protein which promotes long cellular processes in over-expressing cells. Genes Cells 6(11):955–966. doi:475[pii] CrossRefPubMed
40.
Zurück zum Zitat Degreve B, Johansson M, De Clercq E, Karlsson A, Balzarini J (1998) Differential intracellular compartmentalization of herpetic thymidine kinases (TKs) in TK gene-transfected tumor cells: molecular characterization of the nuclear localization signal of herpes simplex virus type 1 TK. J Virol 72(12):9535–9543PubMed Degreve B, Johansson M, De Clercq E, Karlsson A, Balzarini J (1998) Differential intracellular compartmentalization of herpetic thymidine kinases (TKs) in TK gene-transfected tumor cells: molecular characterization of the nuclear localization signal of herpes simplex virus type 1 TK. J Virol 72(12):9535–9543PubMed
42.
43.
Zurück zum Zitat Goshima F, Watanabe D, Takakuwa H, Wada K, Daikoku T, Yamada M, Nishiyama Y (2000) Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells. Arch Virol 145(2):417–426CrossRefPubMed Goshima F, Watanabe D, Takakuwa H, Wada K, Daikoku T, Yamada M, Nishiyama Y (2000) Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells. Arch Virol 145(2):417–426CrossRefPubMed
44.
Zurück zum Zitat Beitia Ortiz de Zarate I, Kaelin K, Rozenberg F (2004) Effects of mutations in the cytoplasmic domain of herpes simplex virus type 1 glycoprotein B on intracellular transport and infectivity. J Virol 78(3):1540–1551CrossRefPubMed Beitia Ortiz de Zarate I, Kaelin K, Rozenberg F (2004) Effects of mutations in the cytoplasmic domain of herpes simplex virus type 1 glycoprotein B on intracellular transport and infectivity. J Virol 78(3):1540–1551CrossRefPubMed
45.
Zurück zum Zitat Beard PM, Taus NS, Baines JD (2002) DNA cleavage and packaging proteins encoded by genes U(L)28, U(L)15, and U(L)33 of herpes simplex virus type 1 form a complex in infected cells. J Virol 76(10):4785–4791CrossRefPubMed Beard PM, Taus NS, Baines JD (2002) DNA cleavage and packaging proteins encoded by genes U(L)28, U(L)15, and U(L)33 of herpes simplex virus type 1 form a complex in infected cells. J Virol 76(10):4785–4791CrossRefPubMed
47.
Zurück zum Zitat Chang YE, Poon AP, Roizman B (1996) Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J Virol 70(6):3938–3946PubMed Chang YE, Poon AP, Roizman B (1996) Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. J Virol 70(6):3938–3946PubMed
50.
Zurück zum Zitat Olivo PD, Nelson NJ, Challberg MD (1989) Herpes simplex virus type 1 gene products required for DNA replication: identification and overexpression. J Virol 63(1):196–204PubMed Olivo PD, Nelson NJ, Challberg MD (1989) Herpes simplex virus type 1 gene products required for DNA replication: identification and overexpression. J Virol 63(1):196–204PubMed
51.
Zurück zum Zitat Ward PL, Barker DE, Roizman B (1996) A novel herpes simplex virus 1 gene, UL43.5, maps antisense to the UL43 gene and encodes a protein which colocalizes in nuclear structures with capsid proteins. J Virol 70(5):2684–2690PubMed Ward PL, Barker DE, Roizman B (1996) A novel herpes simplex virus 1 gene, UL43.5, maps antisense to the UL43 gene and encodes a protein which colocalizes in nuclear structures with capsid proteins. J Virol 70(5):2684–2690PubMed
54.
Zurück zum Zitat Morrison EE, Stevenson AJ, Wang YF, Meredith DM (1998) Differences in the intracellular localization and fate of herpes simplex virus tegument proteins early in the infection of vero cells. J Gen Virol 79(Pt 10):2517–2528PubMed Morrison EE, Stevenson AJ, Wang YF, Meredith DM (1998) Differences in the intracellular localization and fate of herpes simplex virus tegument proteins early in the infection of vero cells. J Gen Virol 79(Pt 10):2517–2528PubMed
55.
Zurück zum Zitat Blouin A, Blaho JA (2001) Assessment of the subcellular localization of the herpes simplex virus structural protein VP22 in the absence of other viral gene products. Virus Res 81(1–2):57–68. doi:S0168170201003550[pii] CrossRefPubMed Blouin A, Blaho JA (2001) Assessment of the subcellular localization of the herpes simplex virus structural protein VP22 in the absence of other viral gene products. Virus Res 81(1–2):57–68. doi:S016817020100355​0[pii] CrossRefPubMed
56.
Zurück zum Zitat Nozawa N, Daikoku T, Koshizuka T, Yamauchi Y, Yoshikawa T, Nishiyama Y (2003) Subcellular localization of herpes simplex virus type 1 UL51 protein and role of palmitoylation in Golgi apparatus targeting. J Virol 77(5):3204–3216CrossRefPubMed Nozawa N, Daikoku T, Koshizuka T, Yamauchi Y, Yoshikawa T, Nishiyama Y (2003) Subcellular localization of herpes simplex virus type 1 UL51 protein and role of palmitoylation in Golgi apparatus targeting. J Virol 77(5):3204–3216CrossRefPubMed
57.
Zurück zum Zitat Hutchinson L, Roop-Beauchamp C, Johnson DC (1995) Herpes simplex virus glycoprotein K is known to influence fusion of infected cells, yet is not on the cell surface. J Virol 69(7):4556–4563PubMed Hutchinson L, Roop-Beauchamp C, Johnson DC (1995) Herpes simplex virus glycoprotein K is known to influence fusion of infected cells, yet is not on the cell surface. J Virol 69(7):4556–4563PubMed
58.
Zurück zum Zitat Mears WE, Lam V, Rice SA (1995) Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27. J Virol 69(2):935–947PubMed Mears WE, Lam V, Rice SA (1995) Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27. J Virol 69(2):935–947PubMed
59.
Zurück zum Zitat Yamada H, Jiang YM, Oshima S, Daikoku T, Yamashita Y, Tsurumi T, Nishiyama Y (1998) Characterization of the UL55 gene product of herpes simplex virus type 2. J Gen Virol 79(Pt 8):1989–1995PubMed Yamada H, Jiang YM, Oshima S, Daikoku T, Yamashita Y, Tsurumi T, Nishiyama Y (1998) Characterization of the UL55 gene product of herpes simplex virus type 2. J Gen Virol 79(Pt 8):1989–1995PubMed
60.
Zurück zum Zitat Koshizuka T, Goshima F, Takakuwa H, Nozawa N, Daikoku T, Koiwai O, Nishiyama Y (2002) Identification and characterization of the UL56 gene product of herpes simplex virus type 2. J Virol 76(13):6718–6728CrossRefPubMed Koshizuka T, Goshima F, Takakuwa H, Nozawa N, Daikoku T, Koiwai O, Nishiyama Y (2002) Identification and characterization of the UL56 gene product of herpes simplex virus type 2. J Virol 76(13):6718–6728CrossRefPubMed
62.
Zurück zum Zitat Goshima F, Watanabe D, Suzuki H, Takakuwa H, Yamada H, Nishiyama Y (2001) The US2 gene product of herpes simplex virus type 2 interacts with cytokeratin 18. Arch Virol 146(11):2201–2209CrossRefPubMed Goshima F, Watanabe D, Suzuki H, Takakuwa H, Yamada H, Nishiyama Y (2001) The US2 gene product of herpes simplex virus type 2 interacts with cytokeratin 18. Arch Virol 146(11):2201–2209CrossRefPubMed
63.
Zurück zum Zitat Ghiasi H, Nesburn AB, Cai S, Wechsler SL (1998) The US5 open reading frame of herpes simplex virus type 1 does encode a glycoprotein (gJ). Intervirology 41(2–3):91–97. doi:int41091[pii] CrossRefPubMed Ghiasi H, Nesburn AB, Cai S, Wechsler SL (1998) The US5 open reading frame of herpes simplex virus type 1 does encode a glycoprotein (gJ). Intervirology 41(2–3):91–97. doi:int41091[pii] CrossRefPubMed
64.
Zurück zum Zitat Whiteley A, Bruun B, Minson T, Browne H (1999) Effects of targeting herpes simplex virus type 1 gD to the endoplasmic reticulum and trans-Golgi network. J Virol 73(11):9515–9520PubMed Whiteley A, Bruun B, Minson T, Browne H (1999) Effects of targeting herpes simplex virus type 1 gD to the endoplasmic reticulum and trans-Golgi network. J Virol 73(11):9515–9520PubMed
65.
Zurück zum Zitat Georgopoulou U, Kakkanas A, Miriagou V, Michaelidou A, Mavromara P (1995) Characterization of the US8.5 protein of herpes simplex virus. Arch Virol 140(12):2227–2241CrossRefPubMed Georgopoulou U, Kakkanas A, Miriagou V, Michaelidou A, Mavromara P (1995) Characterization of the US8.5 protein of herpes simplex virus. Arch Virol 140(12):2227–2241CrossRefPubMed
66.
Zurück zum Zitat Georgopoulou U, Michaelidou A, Roizman B, Mavromara-Nazos P (1993) Identification of a new transcriptional unit that yields a gene product within the unique sequences of the short component of the herpes simplex virus 1 genome. J Virol 67(7):3961–3968PubMed Georgopoulou U, Michaelidou A, Roizman B, Mavromara-Nazos P (1993) Identification of a new transcriptional unit that yields a gene product within the unique sequences of the short component of the herpes simplex virus 1 genome. J Virol 67(7):3961–3968PubMed
67.
Zurück zum Zitat Diaz-Latoud C, Diaz JJ, Fabre-Jonca N, Kindbeiter K, Madjar JJ, Arrigo AP (1997) Herpes simplex virus US11 protein enhances recovery of protein synthesis and survival in heat shock treated HeLa cells. Cell Stress Chaperones 2(2):119–131CrossRefPubMed Diaz-Latoud C, Diaz JJ, Fabre-Jonca N, Kindbeiter K, Madjar JJ, Arrigo AP (1997) Herpes simplex virus US11 protein enhances recovery of protein synthesis and survival in heat shock treated HeLa cells. Cell Stress Chaperones 2(2):119–131CrossRefPubMed
Metadaten
Titel
Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells
verfasst von
Junji Xing
Shuai Wang
You Li
Hong Guo
Lei Zhao
Weiwei Pan
Fusen Lin
Huifang Zhu
Lei Wang
Meili Li
Lin Wang
Chunfu Zheng
Publikationsdatum
01.02.2011
Verlag
Springer-Verlag
Erschienen in
Medical Microbiology and Immunology / Ausgabe 1/2011
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI
https://doi.org/10.1007/s00430-010-0175-9

Weitere Artikel der Ausgabe 1/2011

Medical Microbiology and Immunology 1/2011 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.