nach oben

Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.11.2008 | Original Paper

Identification of transgelin as a potential novel biomarker for gastric adenocarcinoma based on proteomics technology

verfasst von: Qiaojia Huang, Qingling Huang, Wannan Chen, Lin Wang, Wansong Lin, Jianyin Lin, Xu Lin

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 11/2008

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To find a biomarker for gastric adenocarcinomas (GA).

Methods

Ten protein expression profiles of GA and paired non-neoplastic mucosa tissues were analyzed by two-dimensional gel electrophoresis. Forty-two protein spots that were differentially expressed by twofold or greater between cancer and normal mucosa tissue were excised and identified by MALDI-TOF/TOF MS. One of the over-expressed proteins identified in GA was transgelin, which was chosen for further verification by immunohistochemistry and western blotting.

Results

Forty-two distinct proteins that were differentially expressed at least twofold between the tissues were identified. Expression of 29 of these proteins was decreased (ratio ≥ 2, P < 0.01), including adenosine deaminase; and 13 proteins displayed over-expression in cancer tissue (ratio ≥ 2, P < 0.01), including transgelin. The results of immunohistochemistry confirmed that transgelin was indeed over-expressed in 22 cases of GA (22/41, 53.66%), with strong cytoplasmic staining in cancer cells of positive samples, this was absent in most paired non-neoplastic mucosa cells or gastric ulcer tissues (n = 20). Transgelin was found over-expressed in 21 samples of cancer tissue (21/41, 51.22%) when detected by western blot.

Conclusion

This work demonstrates that differentially expressed proteins can be identified by proteomics technology combined with immunohistochemistry and western blot analyses. We have identified one such protein, transgelin, as a novel biomarker for GA.

Aung PP, Oue N, Mitani Y et al (2006) Systematic search for gastric cancer-specific genes based on SAGE data: melanoma inhibitory activity and matrixmetalloproteinase-10 are novel prognostic factors in patients with gastric cancer. Oncogene 25:2546–2557PubMedCrossRef

Cheng J, Yang J, Xia Y, Karin M, Su B (2000) Synergistic interaction of MEK kinase 2, c-Jun N-terminal kinase (JNK) kinase 2, and JNK1 results in efficient and specific JNK1 activation. Mol Cell Biol 20:2334–2342PubMedCrossRef

Cheung PK, Woolcock B, Adomat H et al (2004) Protein profiling of microdissected prostate tissue links growth differentiation factor 15 to prostate carcinogenesis. Cancer Res 64:5929–5933PubMedCrossRef

Cho WC, Cheng CH (2007) Oncoproteomics: current trends and future perspectives. Expert Rev Proteomics 4:401–410PubMedCrossRef

Dube V, Grigull J, Desouza LV et al (2007) Verification of endometrial tissue biomarkers previously discovered using mass spectrometry-based proteomics by means of immunohistochemistry in a tissue microarray format. J Proteome Res 6:2648–2655PubMedCrossRef

Ebert MP, Krüger S, Fogeron ML et al (2005) Overexpression of cathepsin B in gastric cancer identified by proteome analysis. Proteomics 5:1693–1704PubMedCrossRef

Fu Y, Liu HW, Forsythe SM et al (2000) Mutagenesis analysis of human SM22: characterization of actin binding. J Appl Physiol 89:1985–1990PubMed

Goodman A, Goode BL, Matsudaira P, Fink GR (2003) The saccharomyces cerevisiae calponin/transgelin homolog scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton. Mol Biol Cell 14:2617–2629PubMedCrossRef

Humphery-Smith I, Cordwell SJ, Blackstock WP (1997) Proteome research: complementarity and limitations with respect to the RNA and DNA worlds. J Electrophor 18:1217–1242CrossRef

Lawson D, Harrison M, Shapland C (1998) Fibroblast transgelin and smooth muscle SM22α are the same protein, the expression of which is down-regulated in many cell lines. Cell Motil Cytoskeleton 38:250–257CrossRef

Li X, Zhuang ZX, Liu JJ, Huang JJ, Wei HY, Qin Z, Yang XH (2006) Proteomic analysis to identify the cellular responses induced by hydroquinone in human embryonic lung fibroblasts. Toxicol Mech Methods 16:1–6CrossRef

Melle C, Ernst G, Schimmel B et al (2005) Characterization of pepsinogen C as a potential biomarker for gastric cancer using a histo-proteomic approach. J Proteome Res 4:1799–1804PubMedCrossRef

Menice CB, Hulvershorn J, Adam LP, Wang CA, Morgan KG (1997) Calponin and mitogen-activated protein kinase signaling in differentiated vascular smooth muscle. J Biol Chem 272:25157–25161PubMedCrossRef

Mikuriya K, Kuramitsu Y, Ryozawa S et al (2007) Expression of glycolytic enzymes is increased in pancreatic cancerous tissues as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography–mass spectrometry/mass spectrometry. Int J Oncol 30:849–855PubMed

Nair RR, Solway J, Boyd DD (2006) Expression cloning identifies transgelin (SM22) as a novel repressor of 92-kDa type IV collagenase (MMP–9) expression. J Biol Chem 281:26424–26436PubMedCrossRef

Ohgaki H, Matsukura N (2003) Stomach cancer. In: Stewart BW, Kleihues P (eds) World cancer report. IARC Press, Lyon, pp 197–201

Prinjha RK, Shapland CE, Hsuan JJ, Totty NF, Mason IJ, Lawson D (1994) Cloning and sequencing of cDNAs encoding the actin cross-linking protein transgelin defines a new family of actin-associated proteins. Cell Motil Cytoskeleton 28:243–255PubMedCrossRef

Roessler M, Rollinger W, Mantovani EL et al (2006) Identification of PSME3 as a novel serum tumor marker for colorectal cancer by combining two-dimensional polyacrylamide gel electrophoresis with a strictly mass spectrometry-based approach for data. Anal Mol Cell Proteomics 5:2091–2101

Roeßler M, Rollinger W, Palme S et al (2005) Identification of nicotinamide N-methyltransferase as a novel serum tumor marker for colorectal cancer. Clin Cancer Res 11:6550–6557PubMedCrossRef

Rosty C, Christa L, Kuzdzal S et al (2002) Identification of hepatocarcinoma–intestine–pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. Cancer Res 62:1868–1875PubMed

Shi YY, Wang HC, Yin YH et al (2005) Identification and analysis of tumour-associated antigens in hepatocellular carcinoma. Br J Cancer 92:929–934PubMedCrossRef

Shields JM, Rogers-Graham K, Der CJ (2002) Loss of transgelin in breast and colon tumors and in RIE-1 cells by Ras deregulation of gene expression through Raf-independent pathways. J Biol Chem 277:9790–9799PubMedCrossRef

Sidransky D. (2002) Emerging molecular markers of cancer. Nat Rev Cancer 2:210–219PubMedCrossRef

Solway J, Seltzer J, Samaha FF et al (1995) Structure and expression of a smooth muscle cell-specific gene, SM22 alpha. J Biol Chem 270:13460–13469PubMedCrossRef

Srinivas PR, Kramer BS, Srivastava S (2001) Trends in biomarker research for cancer detection. Lancet Oncol 2:698–704PubMedCrossRef

Ushijima T, Sasako M (2004) Focus on gastric cancer. Cancer Cell 5:121–125PubMedCrossRef

van’t Veer LJ, Dai HY, van de Vijver MJ et al (2002) Gene expression profiling predicts clinical outcome of breast cancer. Nature 415:530–536CrossRef

Wilkins MR, Sanchez JC, Gooley AA et al (1996) Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Gen Eng Rev 13:19–50

Xing X, Lai M, Gartner W et al (2006) Identification of differentially expressed proteins in colorectal cancer by proteomics: down-regulation of secretagogin. Proteomics 6:2916–2923PubMedCrossRef

Yang Z, Chang YJ, Miyamoto H et al (2007) Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth. Mol Endocrinol 21:343–358PubMedCrossRef

Yasui W, Yokozaki H, Fujimoto J, Naka K, Kuniyasu H, Tahara E (2000) Genetic and epigenetic alterations in multistep carcinogenesis of the stomach. J Gastroenterol 35:111–115PubMed

Titel: Identification of transgelin as a potential novel biomarker for gastric adenocarcinoma based on proteomics technology
verfasst von: Qiaojia Huang
Qingling Huang
Wannan Chen
Lin Wang
Wansong Lin
Jianyin Lin
Xu Lin
Publikationsdatum: 01.11.2008
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 11/2008
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-008-0398-y

Neu im Fachgebiet Onkologie

18.04.2024 | Wirbelsäulenmetastase | Nachrichten

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Identification of transgelin as a potential novel biomarker for gastric adenocarcinoma based on proteomics technology

Abstract

Purpose

Methods

Results

Conclusion

Neu im Fachgebiet Onkologie

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Einladung zum PSA-Screening senkt die Krebssterblichkeit

Update Onkologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 11/2008

Germline mutations in the von Hippel–Lindau disease (VHL) gene in mainland Chinese families

Three-hit hypothesis in astrocytoma: tracing the polymorphism D1853N in ATM gene through a pedigree of the proband affected with primary brain tumor

Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials

Establishment of a new method, transcription–reverse transcription concerted reaction, for detection of plasma hnRNP B1 mRNA, a biomarker of lung cancer

Establishment of CXCR4-small interfering RNA retrovirus vector driven by human prostate-specific antigen promoter and its biological effects on prostate cancer in vitro and in vivo

Differential expression of cancer-related genes by single and permanent exposure to bone morphogenetic protein 2

Neu im Fachgebiet Onkologie

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Einladung zum PSA-Screening senkt die Krebssterblichkeit

Update Onkologie