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Erschienen in: Journal of Cancer Research and Clinical Oncology 11/2011

01.11.2011 | Original Paper

Identification of CD44+CD24+ gastric cancer stem cells

verfasst von: Chaojun Zhang, Chenwei Li, Fengtian He, Yujiao Cai, Hua Yang

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 11/2011

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Abstract

Objective

Purification and characterization of cancer stem cells (CSCs) can lead to the identification of targets for therapeutic interventions of cancer. With regard to gastric cancer, studies have not yet defined and characterized CSCs.

Methods

The expression of the cell surface markers CD44 and CD24 was examined in gastric cell lines AGS and gastric cancer tissues from five patients with fluorescence-activated cell sorting analysis (FACS). The tumorigenic properties, self-renewal, and differentiated progeny in the two distinct cell populations CD44+CD24+ and CD44−CD24− were identified in vivo serial transplantation and in vitro culture. Real-time RT-PCR was used to assess the expression of sonic hedgehog (SHH), patched 1 (PTCH1), and GLI3 signaling molecules in CD44+CD24+ and CD44−CD24− cells.

Results

As few as 200 CD44+CD24+ cells injected in NOD–SCID mice were able to generate tumors in 50% of mice (6 of 12), while tumors did not form in mice until at least 10,000 CD44−CD24− cells were injected, where only one of 12 mice formed a tumor, further verifying that CD44+CD24+ gastric cancer cells have the capacity to both self-renew and produce differentiated progeny. Moreover, SHH, PTCH1, and GLI3 mRNA expression increased significantly in the CD44+CD24+ subpopulation when compared with the CD44−CD24− subpopulation.

Conclusions

These studies strongly suggest that the CD44+CD24+ subpopulation of human gastric cancer cell lines, AGS, is gastric cancer stem cells.
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Metadaten
Titel
Identification of CD44+CD24+ gastric cancer stem cells
verfasst von
Chaojun Zhang
Chenwei Li
Fengtian He
Yujiao Cai
Hua Yang
Publikationsdatum
01.11.2011
Verlag
Springer-Verlag
Erschienen in
Journal of Cancer Research and Clinical Oncology / Ausgabe 11/2011
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-011-1038-5

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