Skip to main content
Erschienen in: Journal of Cancer Research and Clinical Oncology 3/2014

01.03.2014 | Original Paper

Effects of resveratrol on oral squamous cell carcinoma (OSCC) cells in vitro

verfasst von: Zhou Shan, Guo Yang, Wang Xiang, Wang Pei-jun, Zhang Bin

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 3/2014

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Resveratrol has been reported to exhibit anti-cancer activity. The aim of this study was to examine the effects of resveratrol on the adhesion, migration, and invasion of the oral squamous cell carcinoma (OSCC) cell line KB cell in vitro.

Methods

The effect of resveratrol on KB cell adhesion was evaluated by a MTT colorimetric assay, whereas its effects on cell migration and invasion were assessed by a Transwell assay.

Results

The MTT assay revealed that the adhesion of KB cells treated with 100 μmol resveratrol for 1 or 2 h was decreased by 49.92 and 58.21 %, respectively (P < 0.05). In the Transwell assay, the migratory and invasive abilities of KB cells treated with 100 μmol resveratrol were decreased by 43.98 and 37.69 %, respectively.

Conclusions

Resveratrol inhibited the adhesion, migration, and invasion of OSCC cells in vitro, suggesting that it might serve as a chemopreventive agent for reducing the invasion and metastasis of OSCC.
Literatur
Zurück zum Zitat Athar M, Back JH, Kopelovich L et al (2009) Multiple molecular target of resveratrol: anti-carcinogenic mechanisms. Arch Biochem Biophys 486:95–102PubMedCentralPubMedCrossRef Athar M, Back JH, Kopelovich L et al (2009) Multiple molecular target of resveratrol: anti-carcinogenic mechanisms. Arch Biochem Biophys 486:95–102PubMedCentralPubMedCrossRef
Zurück zum Zitat Colin D, Gimazane A, Lizard G, Izard JC, Solary E, Latruffe N et al (2009) Effects of resveratrol analogs on cell cycle progression, cell cycle associated proteins and 5 fluoro-uracil sensitivity in human derived colon cancer cells. Int J Cancer 124:2780–2788PubMedCrossRef Colin D, Gimazane A, Lizard G, Izard JC, Solary E, Latruffe N et al (2009) Effects of resveratrol analogs on cell cycle progression, cell cycle associated proteins and 5 fluoro-uracil sensitivity in human derived colon cancer cells. Int J Cancer 124:2780–2788PubMedCrossRef
Zurück zum Zitat Felding-Habermann B (2003) Integrin adhesion receptors in tumor metastasis. Clin Exp Metastasis 20:203–213PubMedCrossRef Felding-Habermann B (2003) Integrin adhesion receptors in tumor metastasis. Clin Exp Metastasis 20:203–213PubMedCrossRef
Zurück zum Zitat Fontecave M, Lepoivre M, Elleingand E, Gerez C, Guittet O (1998) Resveratrol, a remarkable inhibitor of ribonucleotide reductase. FEBS Lett 421:277–279PubMedCrossRef Fontecave M, Lepoivre M, Elleingand E, Gerez C, Guittet O (1998) Resveratrol, a remarkable inhibitor of ribonucleotide reductase. FEBS Lett 421:277–279PubMedCrossRef
Zurück zum Zitat Harper CE, Patel BB, Wang J, Arabshahi A, Eltoum IA, Lamartiniere CA (2007) Resveratrol suppresses prostate cancer progression in transgenic mice. Carcinogenesis 28:1946–1953PubMedCrossRef Harper CE, Patel BB, Wang J, Arabshahi A, Eltoum IA, Lamartiniere CA (2007) Resveratrol suppresses prostate cancer progression in transgenic mice. Carcinogenesis 28:1946–1953PubMedCrossRef
Zurück zum Zitat Kowalski LP, Bagietto R, Lara JR et al (2003) Prognoslic signilicance of the distribution of neck metastasis from oral carcinoma. Herd Neck 22:207–214CrossRef Kowalski LP, Bagietto R, Lara JR et al (2003) Prognoslic signilicance of the distribution of neck metastasis from oral carcinoma. Herd Neck 22:207–214CrossRef
Zurück zum Zitat Lauffenburger DA, Horwitz AF (1996) Cell migration: a physically integrated molecular process. Cell 84:359–369PubMedCrossRef Lauffenburger DA, Horwitz AF (1996) Cell migration: a physically integrated molecular process. Cell 84:359–369PubMedCrossRef
Zurück zum Zitat Li XM, Di B, YD S et al (2005) Clinicopathologic features and prognostic factors of cervical lymph node metastasis in oral squamous cell carcinoma. Chin J Cancer 24:208–212 Li XM, Di B, YD S et al (2005) Clinicopathologic features and prognostic factors of cervical lymph node metastasis in oral squamous cell carcinoma. Chin J Cancer 24:208–212
Zurück zum Zitat Li Y, Xu Y, Huang D, Jiang L, Li K (2007) Inhibit effection of resveratrol on the growth of Hep-2 cell line. J Clin Otorhinolaryngol Head Neck Surg 21:1129–1131 Li Y, Xu Y, Huang D, Jiang L, Li K (2007) Inhibit effection of resveratrol on the growth of Hep-2 cell line. J Clin Otorhinolaryngol Head Neck Surg 21:1129–1131
Zurück zum Zitat Lin HY, Lansing L, Merillon JM, Davis FB, Tang HY, Shih A et al (2006) Integrin alphaVbeta3 contains a receptor site for resveratrol. Faseb J 20:1742–1744PubMedCrossRef Lin HY, Lansing L, Merillon JM, Davis FB, Tang HY, Shih A et al (2006) Integrin alphaVbeta3 contains a receptor site for resveratrol. Faseb J 20:1742–1744PubMedCrossRef
Zurück zum Zitat Lin HY, Tang HY, Keating T, Wu YH, Shih A, Hammond D et al (2008) Resveratrol is pro-apoptotic and thyroid hormone is anti-apoptotic in glioma cells: both actions are integrin and ERK mediated. Carcinogenesis 29:62–69PubMedCrossRef Lin HY, Tang HY, Keating T, Wu YH, Shih A, Hammond D et al (2008) Resveratrol is pro-apoptotic and thyroid hormone is anti-apoptotic in glioma cells: both actions are integrin and ERK mediated. Carcinogenesis 29:62–69PubMedCrossRef
Zurück zum Zitat Marier JF, Chen K, Prince P et al (2005) Production of exvivo lipopolysaccl1aride induced tumor necrosis factor—alpha, interleukjn-1 beta, and interleukin—is suppressed by trans–resveratrol in a concentration dependent manner. Can J Vet Res 69:151–154PubMedCentralPubMed Marier JF, Chen K, Prince P et al (2005) Production of exvivo lipopolysaccl1aride induced tumor necrosis factor—alpha, interleukjn-1 beta, and interleukin—is suppressed by trans–resveratrol in a concentration dependent manner. Can J Vet Res 69:151–154PubMedCentralPubMed
Zurück zum Zitat Narayanan BA, Narayanan NK, Re GG et al (2003) Differential expression of genes induced by resveratrol in LNCaP cells: P53-mediated molecular targets. Int J Cancer 104:204–212PubMedCrossRef Narayanan BA, Narayanan NK, Re GG et al (2003) Differential expression of genes induced by resveratrol in LNCaP cells: P53-mediated molecular targets. Int J Cancer 104:204–212PubMedCrossRef
Zurück zum Zitat Noguchi M, Kido Y, kubota H et al (1999) Prognostic factors and relative risk for survival in NI-3 oral squamous cell carcinoma: a multivariate analysis using cox’s hazard model. Br J Oral Maxillolac Surg 37:433–437CrossRef Noguchi M, Kido Y, kubota H et al (1999) Prognostic factors and relative risk for survival in NI-3 oral squamous cell carcinoma: a multivariate analysis using cox’s hazard model. Br J Oral Maxillolac Surg 37:433–437CrossRef
Zurück zum Zitat Qin YM, Li K, He TP, Mo LE, Liang NC (2007) Study on the effect of resveratrol on metastasis-associated ability of ovarian carcinoma HO-8910PM cells in vitro. Zhong Yao Cai 30:176–178PubMed Qin YM, Li K, He TP, Mo LE, Liang NC (2007) Study on the effect of resveratrol on metastasis-associated ability of ovarian carcinoma HO-8910PM cells in vitro. Zhong Yao Cai 30:176–178PubMed
Zurück zum Zitat Sun Z, Yao HY (2005) Inhibitory effects of zeaxanthin from corn gluten meal on human oral squamour cell carcinoma. J Food Sci Biotechnol 24:34–37 Sun Z, Yao HY (2005) Inhibitory effects of zeaxanthin from corn gluten meal on human oral squamour cell carcinoma. J Food Sci Biotechnol 24:34–37
Zurück zum Zitat Sun NJ, Woo SH, Cassady JM, Snapka RM (1998) DNA polymerase and topoisomerase II inhibitors from Psoralea corylifolia. J Nat Prod 61:362–366PubMedCrossRef Sun NJ, Woo SH, Cassady JM, Snapka RM (1998) DNA polymerase and topoisomerase II inhibitors from Psoralea corylifolia. J Nat Prod 61:362–366PubMedCrossRef
Zurück zum Zitat Sun CY, Hu Y, Guo T, Wang HF, Zhang XP, He WJ et al (2006) Resveratrol as a novel agent for treatment of multiple myeloma with matrix metalloproteinase inhibitory activity. Acta Pharmacol Sin 27:1447–1452PubMedCrossRef Sun CY, Hu Y, Guo T, Wang HF, Zhang XP, He WJ et al (2006) Resveratrol as a novel agent for treatment of multiple myeloma with matrix metalloproteinase inhibitory activity. Acta Pharmacol Sin 27:1447–1452PubMedCrossRef
Zurück zum Zitat Tang FY, Chiang EP, Sun YC (2008a) Resveratrol inhibits heregulin-beta1-mediated matrix metalloproteinase-9 expression and cell invasion in human breast cancer cells. J Nutr Biochem 19:287–294PubMedCrossRef Tang FY, Chiang EP, Sun YC (2008a) Resveratrol inhibits heregulin-beta1-mediated matrix metalloproteinase-9 expression and cell invasion in human breast cancer cells. J Nutr Biochem 19:287–294PubMedCrossRef
Zurück zum Zitat Tang FY, Su YC, Chen NC, Hsieh HS, Chen KS (2008b) Resveratrol inhibits migration and invasion of human breast-cancer cells. Mol Nutr Food Res 52:683–691PubMedCrossRef Tang FY, Su YC, Chen NC, Hsieh HS, Chen KS (2008b) Resveratrol inhibits migration and invasion of human breast-cancer cells. Mol Nutr Food Res 52:683–691PubMedCrossRef
Zurück zum Zitat Ulrich S, Woltern F, Stein JM (2005) Molecuar mechanisms of the chemopreventive effects of resveratrol and its analogs in carcinogenesis. Mol Nutr Food Res 49:452–461PubMedCrossRef Ulrich S, Woltern F, Stein JM (2005) Molecuar mechanisms of the chemopreventive effects of resveratrol and its analogs in carcinogenesis. Mol Nutr Food Res 49:452–461PubMedCrossRef
Zurück zum Zitat Waas ET, Wobbes T, Lomme RM, DeGroot J, Ruers T, Hendriks T (2003) Matrix metalloproteinase 2 and 9 activity in patients with colorectal cancer liver metastasis. Br J Surg 90:1556–1564PubMedCrossRef Waas ET, Wobbes T, Lomme RM, DeGroot J, Ruers T, Hendriks T (2003) Matrix metalloproteinase 2 and 9 activity in patients with colorectal cancer liver metastasis. Br J Surg 90:1556–1564PubMedCrossRef
Zurück zum Zitat Woo JH, Lim JH, Kim YH, Suh SI, Min DS, Chang JS et al (2004) Resveratrol inhibits phorbol myristate acetate-induced matrix metalloproteinase-9 expression by inhibiting JNK and PKC delta signal transduction. Oncogene 23:1845–1853PubMedCrossRef Woo JH, Lim JH, Kim YH, Suh SI, Min DS, Chang JS et al (2004) Resveratrol inhibits phorbol myristate acetate-induced matrix metalloproteinase-9 expression by inhibiting JNK and PKC delta signal transduction. Oncogene 23:1845–1853PubMedCrossRef
Zurück zum Zitat Wu H, Liang X, Fang Y, Qin X, Zhang Y, Liu J (2008) Resveratrol inhibits hypoxia-induced metastasis potential enhancement by restricting hypoxia-induced factor-1 alpha expression in colon carcinoma cells. Biomed Pharmacother 62:613–621PubMedCrossRef Wu H, Liang X, Fang Y, Qin X, Zhang Y, Liu J (2008) Resveratrol inhibits hypoxia-induced metastasis potential enhancement by restricting hypoxia-induced factor-1 alpha expression in colon carcinoma cells. Biomed Pharmacother 62:613–621PubMedCrossRef
Zurück zum Zitat Xu YP, Zhao XQ, Sommer K, Moubayed P (2003) Correlation of matrix metalloproteinase-2, -9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis. J Zhejiang Univ Sci 4:491–501PubMedCrossRef Xu YP, Zhao XQ, Sommer K, Moubayed P (2003) Correlation of matrix metalloproteinase-2, -9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis. J Zhejiang Univ Sci 4:491–501PubMedCrossRef
Zurück zum Zitat Yang Z, Zhang B, Mi L (2011) The research of anti-proliferation and induction of apoptosis of resveratrol on human oral squamous cell carcinoma KB cells in vitro. LiaoNing J Tradit Chin Med 38:740–743 Yang Z, Zhang B, Mi L (2011) The research of anti-proliferation and induction of apoptosis of resveratrol on human oral squamous cell carcinoma KB cells in vitro. LiaoNing J Tradit Chin Med 38:740–743
Zurück zum Zitat Zhao R, Jia RD, Wang HJ (2011) Effects of paclitaxel on growth inhibition and pro-apoptosis of human carcinoma of mouth floor KB cells. Chin J Exp Tradit Med 17:177–180 Zhao R, Jia RD, Wang HJ (2011) Effects of paclitaxel on growth inhibition and pro-apoptosis of human carcinoma of mouth floor KB cells. Chin J Exp Tradit Med 17:177–180
Metadaten
Titel
Effects of resveratrol on oral squamous cell carcinoma (OSCC) cells in vitro
verfasst von
Zhou Shan
Guo Yang
Wang Xiang
Wang Pei-jun
Zhang Bin
Publikationsdatum
01.03.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Journal of Cancer Research and Clinical Oncology / Ausgabe 3/2014
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-013-1575-1

Weitere Artikel der Ausgabe 3/2014

Journal of Cancer Research and Clinical Oncology 3/2014 Zur Ausgabe

Update Onkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.