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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.03.2014 | Original Paper

miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma

verfasst von: Ruijing Lu, Ziliang Ji, Xiaoqing Li, Qingna Zhai, Chunjuan Zhao, Zhimao Jiang, Shiqiang Zhang, Liping Nie, Zhendong Yu

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 3/2014

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Abstract

Purpose

Abnormal expression of miRNAs is closely related to a variety of human cancers. The purpose of this study is to identify new tumor suppressor miRNA and elucidate its physiological function and mechanism in renal cell carcinoma (RCC).

Methods

The expression of miR-145 in 45 RCC and adjacent normal tissues was performed by quantitative RT-PCR. Cell proliferation, migration, invasion, apoptosis and cycle assays were carried out for functional analysis after miR-145 transfection. Two target genes of miR-145 were identified by luciferase reporter assay. The altered expression of 84 epithelial to mesenchymal transition (EMT)-related genes after miR-145 transfection was detected by RT² Profiler EMT PCR array.

Results

The expression of miR-145 was downregulated in RCC compared to their normal adjacent tissues. Restoring miR-145 expression in RCC cell lines dramatically suppressed cell proliferation, migration and invasion, and induced cell apoptosis and G2-phase arrest. We further validated those miR-145 targets two oncogenes, ANGPT2 and NEDD9 in RCC. In addition, miR-145 was found to regulate numerous genes involved in the EMT.

Conclusions

These findings demonstrate that miR-145 functions as tumor suppressor in RCC, suggesting that miR-145 may be a potential therapeutic target for RCC.

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Titel: miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma
verfasst von: Ruijing Lu
Ziliang Ji
Xiaoqing Li
Qingna Zhai
Chunjuan Zhao
Zhimao Jiang
Shiqiang Zhang
Liping Nie
Zhendong Yu
Publikationsdatum: 01.03.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 3/2014
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-013-1577-z

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miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma

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Purpose

Methods

Results

Conclusions

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Abstract

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Methods

Results

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