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Journal of Cancer Research and Clinical Oncology

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01.04.2014 | Original Article – Cancer Research

GPER functions as a tumor suppressor in MCF-7 and SK-BR-3 breast cancer cells

verfasst von: Christine Weißenborn, Tanja Ignatov, Angela Poehlmann, Anja K. Wege, Serban D. Costa, Ana Claudia Zenclussen, Atanas Ignatov

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2014

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Abstract

Purpose

The orphan, membrane-bound estrogen receptor (GPER) is expressed at high levels in a large fraction of breast cancer patients, and its expression is favorable for patients’ survival. We investigated the role of GPER as a potential tumor suppressor in MCF-7 and SK-BR-3 breast cancer cells.

Methods

The effect of GPER agonist G-1 in cell culture was used to determine whether GPER inhibit cell growth. The methylation status of GPER promoter was investigated by methylation-specific PCR.

Results

GPER-specific agonist G-1 inhibited breast cancer cell proliferation in concentration-dependent manner via induction of the cell cycle arrest in M-phase, enhanced phosphorylation of histone 3 and cell apoptosis. Analysis of the methylation status of the GPER promoter in MCF-7 and SK-BR-3 cells revealed that GPER expression is regulated by epigenetic mechanisms and GPER expression is inactivated by promoter methylation. Overall, our results are consistent with our recent findings in triple-negative breast cancer cells, and the cell surface expression of GPER makes it an excellent potential therapeutic target for non-triple-negative breast cancer.

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Titel: GPER functions as a tumor suppressor in MCF-7 and SK-BR-3 breast cancer cells
verfasst von: Christine Weißenborn
Tanja Ignatov
Angela Poehlmann
Anja K. Wege
Serban D. Costa
Ana Claudia Zenclussen
Atanas Ignatov
Publikationsdatum: 01.04.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2014
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-014-1598-2

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GPER functions as a tumor suppressor in MCF-7 and SK-BR-3 breast cancer cells

Abstract

Purpose

Methods

Results

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Springer Medizin

Abstract

Purpose

Methods

Results

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